Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1286 and Pf6N2E2_1287 are separated by 26 nucleotides Pf6N2E2_1287 and Pf6N2E2_1288 are separated by 456 nucleotides
Pf6N2E2_1286 - Hemolysin activation/secretion protein associated with VreARI signalling system, at 1,516,167 to 1,517,771
_1286
Pf6N2E2_1287 - Mobile element protein, at 1,517,798 to 1,518,400
_1287
Pf6N2E2_1288 - Protein containing transglutaminase-like domain, putative cysteine protease, at 1,518,857 to 1,519,654
_1288
Position (kb)
1517
1518
1519 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1516.903 kb on + strand, within Pf6N2E2_1286 at 1516.921 kb on + strand, within Pf6N2E2_1286 at 1516.922 kb on - strand, within Pf6N2E2_1286 at 1516.922 kb on - strand, within Pf6N2E2_1286 at 1517.122 kb on + strand, within Pf6N2E2_1286 at 1517.122 kb on + strand, within Pf6N2E2_1286 at 1517.167 kb on + strand, within Pf6N2E2_1286 at 1517.167 kb on + strand, within Pf6N2E2_1286 at 1517.386 kb on + strand, within Pf6N2E2_1286 at 1517.387 kb on - strand, within Pf6N2E2_1286 at 1518.039 kb on + strand, within Pf6N2E2_1287 at 1518.040 kb on - strand, within Pf6N2E2_1287 at 1518.040 kb on - strand, within Pf6N2E2_1287 at 1518.054 kb on + strand, within Pf6N2E2_1287 at 1518.092 kb on + strand, within Pf6N2E2_1287 at 1518.093 kb on - strand, within Pf6N2E2_1287 at 1518.401 kb on - strand at 1518.810 kb on - strand at 1518.858 kb on + strand at 1518.859 kb on - strand at 1519.030 kb on - strand, within Pf6N2E2_1288 at 1519.101 kb on + strand, within Pf6N2E2_1288 at 1519.113 kb on + strand, within Pf6N2E2_1288
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,516,903 + Pf6N2E2_1286 0.46 +0.4 1,516,921 + Pf6N2E2_1286 0.47 -0.2 1,516,922 - Pf6N2E2_1286 0.47 -1.4 1,516,922 - Pf6N2E2_1286 0.47 +3.0 1,517,122 + Pf6N2E2_1286 0.60 +0.7 1,517,122 + Pf6N2E2_1286 0.60 +0.4 1,517,167 + Pf6N2E2_1286 0.62 +0.3 1,517,167 + Pf6N2E2_1286 0.62 -0.2 1,517,386 + Pf6N2E2_1286 0.76 -1.4 1,517,387 - Pf6N2E2_1286 0.76 +0.7 1,518,039 + Pf6N2E2_1287 0.40 -0.2 1,518,040 - Pf6N2E2_1287 0.40 -1.4 1,518,040 - Pf6N2E2_1287 0.40 -0.8 1,518,054 + Pf6N2E2_1287 0.42 -0.7 1,518,092 + Pf6N2E2_1287 0.49 +2.0 1,518,093 - Pf6N2E2_1287 0.49 -1.2 1,518,401 - +0.0 1,518,810 - -0.2 1,518,858 + -1.6 1,518,859 - +0.7 1,519,030 - Pf6N2E2_1288 0.22 +0.4 1,519,101 + Pf6N2E2_1288 0.31 +0.9 1,519,113 + Pf6N2E2_1288 0.32 -0.9
Or see this region's nucleotide sequence