Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1210 and Pf6N2E2_1211 are separated by 125 nucleotides Pf6N2E2_1211 and Pf6N2E2_1212 are separated by 187 nucleotides
Pf6N2E2_1210 - putative aminotransferase, at 1,416,869 to 1,418,017
_1210
Pf6N2E2_1211 - Lactoylglutathione lyase, at 1,418,143 to 1,418,547
_1211
Pf6N2E2_1212 - Oxidoreductase, short chain dehydrogenase/reductase family, at 1,418,735 to 1,419,592
_1212
Position (kb)
1418
1419 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1417.485 kb on + strand, within Pf6N2E2_1210 at 1417.485 kb on + strand, within Pf6N2E2_1210 at 1417.485 kb on + strand, within Pf6N2E2_1210 at 1417.485 kb on + strand, within Pf6N2E2_1210 at 1417.485 kb on + strand, within Pf6N2E2_1210 at 1417.486 kb on - strand, within Pf6N2E2_1210 at 1417.692 kb on - strand, within Pf6N2E2_1210 at 1417.693 kb on + strand, within Pf6N2E2_1210 at 1417.730 kb on + strand, within Pf6N2E2_1210 at 1417.730 kb on + strand, within Pf6N2E2_1210 at 1417.752 kb on - strand, within Pf6N2E2_1210 at 1417.814 kb on + strand, within Pf6N2E2_1210 at 1417.815 kb on - strand, within Pf6N2E2_1210 at 1418.144 kb on + strand at 1418.145 kb on - strand at 1418.145 kb on - strand at 1418.217 kb on - strand, within Pf6N2E2_1211 at 1418.493 kb on - strand, within Pf6N2E2_1211 at 1418.533 kb on + strand at 1418.534 kb on - strand at 1418.534 kb on - strand at 1418.534 kb on - strand at 1418.534 kb on - strand at 1418.573 kb on + strand at 1418.574 kb on - strand at 1418.574 kb on - strand at 1418.574 kb on - strand at 1418.580 kb on - strand at 1418.644 kb on - strand at 1418.646 kb on + strand at 1418.646 kb on + strand at 1418.647 kb on - strand at 1418.647 kb on - strand at 1418.834 kb on + strand, within Pf6N2E2_1212 at 1418.834 kb on + strand, within Pf6N2E2_1212 at 1418.834 kb on + strand, within Pf6N2E2_1212 at 1419.095 kb on - strand, within Pf6N2E2_1212 at 1419.154 kb on - strand, within Pf6N2E2_1212 at 1419.175 kb on + strand, within Pf6N2E2_1212 at 1419.175 kb on + strand, within Pf6N2E2_1212 at 1419.175 kb on + strand, within Pf6N2E2_1212 at 1419.176 kb on - strand, within Pf6N2E2_1212 at 1419.305 kb on + strand, within Pf6N2E2_1212 at 1419.306 kb on - strand, within Pf6N2E2_1212 at 1419.306 kb on - strand, within Pf6N2E2_1212 at 1419.512 kb on + strand at 1419.512 kb on + strand at 1419.542 kb on + strand at 1419.542 kb on + strand at 1419.542 kb on + strand at 1419.542 kb on + strand at 1419.542 kb on + strand at 1419.543 kb on - strand at 1419.543 kb on - strand at 1419.543 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,417,485 + Pf6N2E2_1210 0.54 -0.4 1,417,485 + Pf6N2E2_1210 0.54 -0.8 1,417,485 + Pf6N2E2_1210 0.54 +0.0 1,417,485 + Pf6N2E2_1210 0.54 +0.1 1,417,485 + Pf6N2E2_1210 0.54 -0.9 1,417,486 - Pf6N2E2_1210 0.54 +0.2 1,417,692 - Pf6N2E2_1210 0.72 +0.9 1,417,693 + Pf6N2E2_1210 0.72 -0.9 1,417,730 + Pf6N2E2_1210 0.75 -0.1 1,417,730 + Pf6N2E2_1210 0.75 -0.9 1,417,752 - Pf6N2E2_1210 0.77 +0.5 1,417,814 + Pf6N2E2_1210 0.82 +1.0 1,417,815 - Pf6N2E2_1210 0.82 +0.2 1,418,144 + -1.2 1,418,145 - -0.9 1,418,145 - -1.0 1,418,217 - Pf6N2E2_1211 0.18 -2.1 1,418,493 - Pf6N2E2_1211 0.86 -2.2 1,418,533 + +0.5 1,418,534 - +0.4 1,418,534 - -1.2 1,418,534 - -0.7 1,418,534 - +0.3 1,418,573 + -1.9 1,418,574 - +0.1 1,418,574 - -0.1 1,418,574 - -0.5 1,418,580 - -0.0 1,418,644 - +0.8 1,418,646 + +0.3 1,418,646 + +1.8 1,418,647 - +0.0 1,418,647 - -0.7 1,418,834 + Pf6N2E2_1212 0.12 +1.6 1,418,834 + Pf6N2E2_1212 0.12 +1.4 1,418,834 + Pf6N2E2_1212 0.12 +0.3 1,419,095 - Pf6N2E2_1212 0.42 +1.2 1,419,154 - Pf6N2E2_1212 0.49 -0.2 1,419,175 + Pf6N2E2_1212 0.51 +0.6 1,419,175 + Pf6N2E2_1212 0.51 +1.4 1,419,175 + Pf6N2E2_1212 0.51 +1.4 1,419,176 - Pf6N2E2_1212 0.51 +1.1 1,419,305 + Pf6N2E2_1212 0.66 -0.7 1,419,306 - Pf6N2E2_1212 0.67 -2.0 1,419,306 - Pf6N2E2_1212 0.67 +1.1 1,419,512 + +0.4 1,419,512 + +0.2 1,419,542 + -1.0 1,419,542 + +0.4 1,419,542 + +0.4 1,419,542 + +0.1 1,419,542 + +0.5 1,419,543 - +1.3 1,419,543 - -0.9 1,419,543 - -0.5
Or see this region's nucleotide sequence