Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1151 and Pf6N2E2_1152 are separated by 94 nucleotides Pf6N2E2_1152 and Pf6N2E2_1153 are separated by 3 nucleotides Pf6N2E2_1153 and Pf6N2E2_1154 are separated by 77 nucleotides
Pf6N2E2_1151 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 1,348,712 to 1,350,091
_1151
Pf6N2E2_1152 - hypothetical protein, at 1,350,186 to 1,350,920
_1152
Pf6N2E2_1153 - small molecule metabolism; degradation; fatty acid, at 1,350,924 to 1,351,739
_1153
Pf6N2E2_1154 - FIG00978082: hypothetical protein, at 1,351,817 to 1,352,404
_1154
Position (kb)
1350
1351 Strain fitness (log2 ratio)
-2
-1
0
1 at 1349.322 kb on + strand, within Pf6N2E2_1151 at 1349.323 kb on - strand, within Pf6N2E2_1151 at 1349.422 kb on + strand, within Pf6N2E2_1151 at 1349.460 kb on + strand, within Pf6N2E2_1151 at 1349.460 kb on + strand, within Pf6N2E2_1151 at 1349.460 kb on + strand, within Pf6N2E2_1151 at 1349.460 kb on + strand, within Pf6N2E2_1151 at 1349.576 kb on + strand, within Pf6N2E2_1151 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1350.060 kb on - strand at 1350.060 kb on - strand at 1350.060 kb on - strand at 1350.064 kb on - strand at 1350.064 kb on - strand at 1350.166 kb on + strand at 1350.191 kb on + strand at 1350.192 kb on - strand at 1350.230 kb on - strand at 1350.230 kb on - strand at 1350.406 kb on + strand, within Pf6N2E2_1152 at 1350.407 kb on - strand, within Pf6N2E2_1152 at 1350.667 kb on + strand, within Pf6N2E2_1152 at 1350.709 kb on + strand, within Pf6N2E2_1152 at 1350.709 kb on + strand, within Pf6N2E2_1152 at 1350.710 kb on - strand, within Pf6N2E2_1152 at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.872 kb on - strand at 1350.925 kb on + strand at 1350.925 kb on + strand at 1350.925 kb on + strand at 1350.925 kb on + strand at 1351.181 kb on - strand, within Pf6N2E2_1153 at 1351.270 kb on + strand, within Pf6N2E2_1153 at 1351.270 kb on + strand, within Pf6N2E2_1153 at 1351.271 kb on - strand, within Pf6N2E2_1153 at 1351.355 kb on - strand, within Pf6N2E2_1153 at 1351.447 kb on + strand, within Pf6N2E2_1153 at 1351.507 kb on + strand, within Pf6N2E2_1153 at 1351.514 kb on - strand, within Pf6N2E2_1153 at 1351.718 kb on + strand at 1351.743 kb on + strand at 1351.743 kb on + strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.747 kb on + strand at 1351.748 kb on - strand at 1351.815 kb on - strand at 1351.904 kb on + strand, within Pf6N2E2_1154 at 1351.905 kb on - strand, within Pf6N2E2_1154
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,349,322 + Pf6N2E2_1151 0.44 -0.4 1,349,323 - Pf6N2E2_1151 0.44 -1.3 1,349,422 + Pf6N2E2_1151 0.51 -1.9 1,349,460 + Pf6N2E2_1151 0.54 +1.2 1,349,460 + Pf6N2E2_1151 0.54 +1.4 1,349,460 + Pf6N2E2_1151 0.54 +0.4 1,349,460 + Pf6N2E2_1151 0.54 +0.4 1,349,576 + Pf6N2E2_1151 0.63 -1.9 1,349,952 + Pf6N2E2_1151 0.90 +0.7 1,349,952 + Pf6N2E2_1151 0.90 +0.1 1,349,952 + Pf6N2E2_1151 0.90 -1.3 1,350,060 - +0.7 1,350,060 - +0.5 1,350,060 - -0.2 1,350,064 - +0.7 1,350,064 - -0.8 1,350,166 + -0.8 1,350,191 + +0.2 1,350,192 - +0.2 1,350,230 - +0.4 1,350,230 - -0.7 1,350,406 + Pf6N2E2_1152 0.30 -0.6 1,350,407 - Pf6N2E2_1152 0.30 -1.4 1,350,667 + Pf6N2E2_1152 0.65 -0.9 1,350,709 + Pf6N2E2_1152 0.71 -1.2 1,350,709 + Pf6N2E2_1152 0.71 -0.9 1,350,710 - Pf6N2E2_1152 0.71 -0.3 1,350,871 + +0.9 1,350,871 + -0.7 1,350,871 + -1.7 1,350,871 + +0.9 1,350,872 - -0.7 1,350,925 + +0.1 1,350,925 + -1.2 1,350,925 + -0.5 1,350,925 + +0.3 1,351,181 - Pf6N2E2_1153 0.31 -0.4 1,351,270 + Pf6N2E2_1153 0.42 -1.0 1,351,270 + Pf6N2E2_1153 0.42 +1.2 1,351,271 - Pf6N2E2_1153 0.43 +0.6 1,351,355 - Pf6N2E2_1153 0.53 -0.6 1,351,447 + Pf6N2E2_1153 0.64 +1.1 1,351,507 + Pf6N2E2_1153 0.71 -0.4 1,351,514 - Pf6N2E2_1153 0.72 +0.9 1,351,718 + -0.5 1,351,743 + +0.7 1,351,743 + -0.3 1,351,744 - -0.6 1,351,744 - -1.3 1,351,744 - +0.3 1,351,744 - +0.2 1,351,744 - +0.7 1,351,747 + -0.6 1,351,748 - -0.7 1,351,815 - +0.3 1,351,904 + Pf6N2E2_1154 0.15 +0.3 1,351,905 - Pf6N2E2_1154 0.15 +0.4
Or see this region's nucleotide sequence