Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_108 and Pf6N2E2_109 are separated by 119 nucleotides Pf6N2E2_109 and Pf6N2E2_110 are separated by 188 nucleotides Pf6N2E2_110 and Pf6N2E2_111 overlap by 17 nucleotides
Pf6N2E2_108 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 152,753 to 154,495
_108
Pf6N2E2_109 - Hydrolases of the alpha/beta superfamily, at 154,615 to 155,490
_109
Pf6N2E2_110 - Cellobiose phosphotransferase system YdjC-like protein, at 155,679 to 156,461
_110
Pf6N2E2_111 - FIG00954746: hypothetical protein, at 156,445 to 157,620
_111
Position (kb) 154
155
156 Strain fitness (log2 ratio) -2
-1
0
1 at 153.710 kb on + strand, within Pf6N2E2_108 at 153.711 kb on - strand, within Pf6N2E2_108 at 153.711 kb on - strand, within Pf6N2E2_108 at 153.711 kb on - strand, within Pf6N2E2_108 at 153.711 kb on - strand, within Pf6N2E2_108 at 153.731 kb on + strand, within Pf6N2E2_108 at 153.731 kb on + strand, within Pf6N2E2_108 at 153.731 kb on + strand, within Pf6N2E2_108 at 153.732 kb on - strand, within Pf6N2E2_108 at 153.732 kb on - strand, within Pf6N2E2_108 at 153.732 kb on - strand, within Pf6N2E2_108 at 153.818 kb on + strand, within Pf6N2E2_108 at 153.818 kb on + strand, within Pf6N2E2_108 at 153.917 kb on + strand, within Pf6N2E2_108 at 153.918 kb on - strand, within Pf6N2E2_108 at 153.945 kb on - strand, within Pf6N2E2_108 at 154.014 kb on - strand, within Pf6N2E2_108 at 154.116 kb on + strand, within Pf6N2E2_108 at 154.383 kb on - strand at 154.436 kb on - strand at 154.493 kb on + strand at 154.493 kb on + strand at 154.493 kb on + strand at 154.493 kb on + strand at 154.493 kb on + strand at 154.494 kb on - strand at 154.494 kb on - strand at 154.731 kb on + strand, within Pf6N2E2_109 at 154.732 kb on - strand, within Pf6N2E2_109 at 154.920 kb on + strand, within Pf6N2E2_109 at 154.920 kb on + strand, within Pf6N2E2_109 at 154.920 kb on + strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 154.921 kb on - strand, within Pf6N2E2_109 at 155.069 kb on + strand, within Pf6N2E2_109 at 155.070 kb on - strand, within Pf6N2E2_109 at 155.070 kb on - strand, within Pf6N2E2_109 at 155.070 kb on - strand, within Pf6N2E2_109 at 155.102 kb on - strand, within Pf6N2E2_109 at 155.138 kb on + strand, within Pf6N2E2_109 at 155.138 kb on + strand, within Pf6N2E2_109 at 155.138 kb on + strand, within Pf6N2E2_109 at 155.235 kb on + strand, within Pf6N2E2_109 at 155.279 kb on + strand, within Pf6N2E2_109 at 155.280 kb on - strand, within Pf6N2E2_109 at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.448 kb on + strand at 155.449 kb on - strand at 155.449 kb on - strand at 155.449 kb on - strand at 155.449 kb on - strand at 155.545 kb on + strand at 155.546 kb on - strand at 155.546 kb on - strand at 155.591 kb on - strand at 155.640 kb on + strand at 155.662 kb on + strand at 155.769 kb on + strand, within Pf6N2E2_110 at 155.769 kb on + strand, within Pf6N2E2_110 at 155.770 kb on - strand, within Pf6N2E2_110 at 155.860 kb on + strand, within Pf6N2E2_110 at 155.860 kb on + strand, within Pf6N2E2_110 at 155.861 kb on - strand, within Pf6N2E2_110 at 155.861 kb on - strand, within Pf6N2E2_110 at 155.861 kb on - strand, within Pf6N2E2_110 at 155.905 kb on + strand, within Pf6N2E2_110 at 155.906 kb on - strand, within Pf6N2E2_110 at 155.906 kb on - strand, within Pf6N2E2_110 at 156.009 kb on + strand, within Pf6N2E2_110 at 156.009 kb on + strand, within Pf6N2E2_110 at 156.010 kb on - strand, within Pf6N2E2_110 at 156.274 kb on + strand, within Pf6N2E2_110 at 156.374 kb on + strand, within Pf6N2E2_110
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 153,710 + Pf6N2E2_108 0.55 +0.2 153,711 - Pf6N2E2_108 0.55 -0.3 153,711 - Pf6N2E2_108 0.55 -0.4 153,711 - Pf6N2E2_108 0.55 +0.2 153,711 - Pf6N2E2_108 0.55 +0.1 153,731 + Pf6N2E2_108 0.56 -0.2 153,731 + Pf6N2E2_108 0.56 -0.2 153,731 + Pf6N2E2_108 0.56 +0.3 153,732 - Pf6N2E2_108 0.56 -1.1 153,732 - Pf6N2E2_108 0.56 -0.7 153,732 - Pf6N2E2_108 0.56 +0.4 153,818 + Pf6N2E2_108 0.61 -0.5 153,818 + Pf6N2E2_108 0.61 -1.0 153,917 + Pf6N2E2_108 0.67 -0.0 153,918 - Pf6N2E2_108 0.67 +0.4 153,945 - Pf6N2E2_108 0.68 +0.2 154,014 - Pf6N2E2_108 0.72 +0.3 154,116 + Pf6N2E2_108 0.78 +1.3 154,383 - +1.0 154,436 - -0.1 154,493 + -0.2 154,493 + -1.0 154,493 + +0.0 154,493 + -0.4 154,493 + +0.4 154,494 - -0.4 154,494 - -1.0 154,731 + Pf6N2E2_109 0.13 +0.4 154,732 - Pf6N2E2_109 0.13 +0.5 154,920 + Pf6N2E2_109 0.35 -0.9 154,920 + Pf6N2E2_109 0.35 +0.5 154,920 + Pf6N2E2_109 0.35 -1.0 154,921 - Pf6N2E2_109 0.35 -0.8 154,921 - Pf6N2E2_109 0.35 +0.4 154,921 - Pf6N2E2_109 0.35 +0.0 154,921 - Pf6N2E2_109 0.35 -1.2 154,921 - Pf6N2E2_109 0.35 -1.3 154,921 - Pf6N2E2_109 0.35 +0.7 155,069 + Pf6N2E2_109 0.52 -0.0 155,070 - Pf6N2E2_109 0.52 -1.2 155,070 - Pf6N2E2_109 0.52 -1.5 155,070 - Pf6N2E2_109 0.52 -0.8 155,102 - Pf6N2E2_109 0.56 -1.1 155,138 + Pf6N2E2_109 0.60 -1.1 155,138 + Pf6N2E2_109 0.60 +0.1 155,138 + Pf6N2E2_109 0.60 -1.4 155,235 + Pf6N2E2_109 0.71 +0.3 155,279 + Pf6N2E2_109 0.76 -1.1 155,280 - Pf6N2E2_109 0.76 +0.6 155,448 + -1.1 155,448 + -0.4 155,448 + -1.4 155,448 + +0.4 155,448 + -1.6 155,448 + +0.4 155,449 - +0.5 155,449 - -0.0 155,449 - +0.3 155,449 - +0.5 155,545 + -1.0 155,546 - -0.6 155,546 - -0.4 155,591 - +0.4 155,640 + +0.3 155,662 + -0.3 155,769 + Pf6N2E2_110 0.11 -0.2 155,769 + Pf6N2E2_110 0.11 +0.7 155,770 - Pf6N2E2_110 0.12 +0.6 155,860 + Pf6N2E2_110 0.23 -0.9 155,860 + Pf6N2E2_110 0.23 +0.9 155,861 - Pf6N2E2_110 0.23 -1.8 155,861 - Pf6N2E2_110 0.23 -1.6 155,861 - Pf6N2E2_110 0.23 -0.6 155,905 + Pf6N2E2_110 0.29 -0.2 155,906 - Pf6N2E2_110 0.29 +0.7 155,906 - Pf6N2E2_110 0.29 -0.5 156,009 + Pf6N2E2_110 0.42 -0.7 156,009 + Pf6N2E2_110 0.42 +0.7 156,010 - Pf6N2E2_110 0.42 +0.8 156,274 + Pf6N2E2_110 0.76 +0.5 156,374 + Pf6N2E2_110 0.89 -0.2
Or see this region's nucleotide sequence