Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1039 and Pf6N2E2_1040 are separated by 328 nucleotides Pf6N2E2_1040 and Pf6N2E2_1041 overlap by 4 nucleotides
Pf6N2E2_1039 - Gluconolactonase, at 1,212,812 to 1,213,738
_1039
Pf6N2E2_1040 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 1,214,067 to 1,215,008
_1040
Pf6N2E2_1041 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 1,215,005 to 1,216,204
_1041
Position (kb)
1214
1215
1216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1213.261 kb on - strand, within Pf6N2E2_1039 at 1213.266 kb on + strand, within Pf6N2E2_1039 at 1213.317 kb on + strand, within Pf6N2E2_1039 at 1213.318 kb on - strand, within Pf6N2E2_1039 at 1213.318 kb on - strand, within Pf6N2E2_1039 at 1213.500 kb on + strand, within Pf6N2E2_1039 at 1213.500 kb on + strand, within Pf6N2E2_1039 at 1213.572 kb on + strand, within Pf6N2E2_1039 at 1213.572 kb on + strand, within Pf6N2E2_1039 at 1213.729 kb on - strand at 1213.769 kb on + strand at 1213.770 kb on - strand at 1213.789 kb on + strand at 1213.789 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1214.075 kb on + strand at 1214.076 kb on - strand at 1214.076 kb on - strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.087 kb on - strand at 1214.087 kb on - strand at 1214.087 kb on - strand at 1214.090 kb on + strand at 1214.090 kb on + strand at 1214.091 kb on - strand at 1214.091 kb on - strand at 1214.091 kb on - strand at 1214.366 kb on + strand, within Pf6N2E2_1040 at 1214.513 kb on - strand, within Pf6N2E2_1040 at 1214.787 kb on - strand, within Pf6N2E2_1040 at 1215.069 kb on + strand at 1215.160 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.410 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.728 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 1,213,261 - Pf6N2E2_1039 0.48 +1.5 1,213,266 + Pf6N2E2_1039 0.49 -0.4 1,213,317 + Pf6N2E2_1039 0.54 -0.1 1,213,318 - Pf6N2E2_1039 0.55 -1.9 1,213,318 - Pf6N2E2_1039 0.55 -2.3 1,213,500 + Pf6N2E2_1039 0.74 +1.1 1,213,500 + Pf6N2E2_1039 0.74 +0.3 1,213,572 + Pf6N2E2_1039 0.82 -0.1 1,213,572 + Pf6N2E2_1039 0.82 -0.3 1,213,729 - -0.2 1,213,769 + +2.0 1,213,770 - -0.5 1,213,789 + -1.0 1,213,789 + +0.5 1,213,951 + -0.0 1,213,951 + +0.1 1,213,951 + +0.6 1,213,951 + +0.1 1,213,951 + +0.6 1,213,951 + -0.3 1,213,951 + +0.4 1,213,951 + -0.6 1,213,951 + -0.2 1,213,951 + +1.0 1,213,951 + -2.1 1,213,951 + +0.8 1,213,951 + -0.1 1,213,951 + -0.1 1,213,951 + +1.0 1,213,951 + -0.4 1,213,951 + -0.4 1,213,951 + -2.2 1,213,951 + +0.2 1,213,951 + +0.1 1,213,951 + -0.2 1,213,951 + +0.0 1,213,951 + +1.7 1,213,951 + -0.5 1,213,951 + +1.6 1,213,951 + -1.3 1,213,951 + +0.5 1,213,951 + -0.0 1,213,952 - +0.4 1,213,952 - -1.3 1,213,952 - -0.2 1,213,952 - +0.6 1,213,952 - -0.9 1,213,952 - +0.5 1,213,952 - +0.6 1,213,952 - +0.4 1,213,952 - +0.7 1,213,952 - -0.7 1,213,952 - -0.9 1,213,952 - +0.2 1,213,952 - +0.3 1,213,952 - -0.5 1,213,952 - -0.8 1,213,952 - -2.8 1,213,952 - +0.7 1,213,952 - -1.1 1,213,952 - +1.8 1,213,952 - +0.4 1,213,952 - +1.0 1,213,952 - -2.1 1,213,952 - +0.0 1,213,952 - -0.4 1,213,952 - +0.4 1,214,075 + -0.2 1,214,076 - -1.8 1,214,076 - -1.7 1,214,086 + +1.9 1,214,086 + +0.2 1,214,086 + +0.1 1,214,086 + -0.4 1,214,086 + -0.6 1,214,086 + -0.2 1,214,086 + +0.2 1,214,087 - +0.3 1,214,087 - -0.5 1,214,087 - -0.9 1,214,090 + +0.3 1,214,090 + -0.0 1,214,091 - -2.8 1,214,091 - -0.6 1,214,091 - +0.8 1,214,366 + Pf6N2E2_1040 0.32 -1.7 1,214,513 - Pf6N2E2_1040 0.47 -0.2 1,214,787 - Pf6N2E2_1040 0.76 -0.6 1,215,069 + +0.2 1,215,160 + Pf6N2E2_1041 0.13 +0.3 1,215,203 + Pf6N2E2_1041 0.17 +1.3 1,215,203 + Pf6N2E2_1041 0.17 +0.4 1,215,203 + Pf6N2E2_1041 0.17 +0.2 1,215,203 + Pf6N2E2_1041 0.17 +0.3 1,215,203 + Pf6N2E2_1041 0.17 -0.5 1,215,203 + Pf6N2E2_1041 0.17 +0.6 1,215,377 + Pf6N2E2_1041 0.31 +1.4 1,215,377 + Pf6N2E2_1041 0.31 -0.3 1,215,377 + Pf6N2E2_1041 0.31 -0.5 1,215,377 + Pf6N2E2_1041 0.31 -1.1 1,215,377 + Pf6N2E2_1041 0.31 +0.8 1,215,377 + Pf6N2E2_1041 0.31 -0.6 1,215,378 - Pf6N2E2_1041 0.31 +0.4 1,215,378 - Pf6N2E2_1041 0.31 +0.2 1,215,378 - Pf6N2E2_1041 0.31 -0.2 1,215,378 - Pf6N2E2_1041 0.31 +0.3 1,215,378 - Pf6N2E2_1041 0.31 -0.2 1,215,410 + Pf6N2E2_1041 0.34 +0.7 1,215,464 + Pf6N2E2_1041 0.38 +0.5 1,215,464 + Pf6N2E2_1041 0.38 -1.8 1,215,464 + Pf6N2E2_1041 0.38 -0.1 1,215,465 - Pf6N2E2_1041 0.38 +1.3 1,215,465 - Pf6N2E2_1041 0.38 +0.4 1,215,465 - Pf6N2E2_1041 0.38 -1.9 1,215,465 - Pf6N2E2_1041 0.38 -0.5 1,215,713 + Pf6N2E2_1041 0.59 -0.4 1,215,713 + Pf6N2E2_1041 0.59 +0.6 1,215,713 + Pf6N2E2_1041 0.59 +0.9 1,215,713 + Pf6N2E2_1041 0.59 -0.4 1,215,728 - +0.2
Or see this region's nucleotide sequence