Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS13535

Experiment: Growth at pH9 and (C) Trisodium citrate

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Growth at pH9 and (C) Trisodium citrate
3,064,735	+		PFLU_RS13525	0.23	-2.1
3,064,735	+		PFLU_RS13525	0.23	+3.3
3,064,849	+		PFLU_RS13525	0.42	+0.2
3,064,849	+		PFLU_RS13525	0.42	+0.4
3,064,850	-		PFLU_RS13525	0.43	-1.7
3,064,850	-		PFLU_RS13525	0.43	-1.6
3,064,895	+		PFLU_RS13525	0.50	+0.7
3,064,895	+		PFLU_RS13525	0.50	+2.3
3,064,895	+		PFLU_RS13525	0.50	-1.3
3,064,896	-		PFLU_RS13525	0.50	-0.8
3,064,921	+		PFLU_RS13525	0.55	-1.4
3,064,921	+		PFLU_RS13525	0.55	+2.7
3,064,921	+		PFLU_RS13525	0.55	+0.8
3,064,922	-		PFLU_RS13525	0.55	-0.7
3,065,020	+		PFLU_RS13525	0.71	+0.5
3,065,021	-		PFLU_RS13525	0.71	-2.0
3,065,021	-		PFLU_RS13525	0.71	+2.0
3,065,021	-		PFLU_RS13525	0.71	-2.1
3,065,065	+		PFLU_RS13525	0.79	+2.9
3,065,066	-		PFLU_RS13525	0.79	-2.8
3,065,297	+				-1.8
3,065,297	+				-0.8
3,065,298	-				-0.6
3,065,298	-				+1.7
3,065,303	+				+0.7
3,065,303	+				+2.3
3,065,303	+				+3.5
3,065,303	+				+2.0
3,065,303	+				+0.7
3,065,303	+				+1.2
3,065,303	+				-0.1
3,065,304	-				-0.6
3,065,304	-				-0.6
3,065,304	-				-0.6
3,065,304	-				-1.4
3,065,358	+		PFLU_RS13530	0.20	+1.2
3,065,358	+		PFLU_RS13530	0.20	+0.7
3,065,358	+		PFLU_RS13530	0.20	-2.4
3,065,358	+		PFLU_RS13530	0.20	-1.4
3,065,358	+		PFLU_RS13530	0.20	+1.5
3,065,358	+		PFLU_RS13530	0.20	-2.3
3,065,359	-		PFLU_RS13530	0.20	+0.2
3,065,359	-		PFLU_RS13530	0.20	+0.6
3,065,359	-		PFLU_RS13530	0.20	-2.5
3,065,359	-		PFLU_RS13530	0.20	-2.5
3,065,359	-		PFLU_RS13530	0.20	-0.7
3,065,359	-		PFLU_RS13530	0.20	-0.4
3,065,359	-		PFLU_RS13530	0.20	+0.6
3,065,365	+		PFLU_RS13530	0.23	+1.7
3,065,365	+		PFLU_RS13530	0.23	+0.2
3,065,497	+		PFLU_RS13530	0.73	-0.6
3,065,497	+		PFLU_RS13530	0.73	-1.0
3,065,498	-		PFLU_RS13530	0.73	-0.3
3,065,498	-		PFLU_RS13530	0.73	-1.3
3,065,512	+		PFLU_RS13530	0.78	-0.8
3,065,512	+		PFLU_RS13530	0.78	-1.4
3,065,512	+		PFLU_RS13530	0.78	-0.8
3,065,512	+		PFLU_RS13530	0.78	+2.3
3,065,513	-		PFLU_RS13530	0.79	+0.7
3,065,513	-		PFLU_RS13530	0.79	-0.8
3,065,513	-		PFLU_RS13530	0.79	-0.9
3,065,575	+				-0.6
3,065,575	+				-1.2
3,065,575	+				+2.4
3,065,576	-				+0.7
3,065,576	-				-2.0
3,065,576	-				-0.6
3,065,576	-				+1.2
3,065,576	-				+1.7
3,065,576	-				+1.4
3,065,576	-				-0.6
3,065,579	+				-2.5
3,065,579	+				+0.2
3,065,580	-				+0.7
3,065,580	-				+1.7
3,065,634	+				-0.5
3,065,634	+				-1.7
3,065,635	-				+1.2
3,065,635	-				-1.3
3,065,671	-				-0.3
3,065,695	+		PFLU_RS13535	0.16	+0.5
3,065,816	+		PFLU_RS13535	0.47	+0.3
3,065,816	+		PFLU_RS13535	0.47	+2.3
3,065,816	+		PFLU_RS13535	0.47	+2.2
3,065,816	+		PFLU_RS13535	0.47	-1.7
3,065,816	+		PFLU_RS13535	0.47	+2.3
3,065,816	+		PFLU_RS13535	0.47	-0.2
3,065,816	+		PFLU_RS13535	0.47	-2.1
3,065,816	+		PFLU_RS13535	0.47	-3.0
3,065,816	+		PFLU_RS13535	0.47	+1.4
3,065,816	+		PFLU_RS13535	0.47	-1.3
3,065,816	+		PFLU_RS13535	0.47	-0.3
3,065,817	-		PFLU_RS13535	0.48	+0.2
3,065,817	-		PFLU_RS13535	0.48	+0.2
3,065,817	-		PFLU_RS13535	0.48	-0.3
3,065,817	-		PFLU_RS13535	0.48	+1.4
3,065,817	-		PFLU_RS13535	0.48	-0.3
3,065,817	-		PFLU_RS13535	0.48	-1.3
3,065,817	-		PFLU_RS13535	0.48	+0.2
3,065,838	+		PFLU_RS13535	0.53	-1.1
3,065,838	+		PFLU_RS13535	0.53	+2.8
3,065,838	+		PFLU_RS13535	0.53	+1.2
3,065,838	+		PFLU_RS13535	0.53	-1.1
3,065,838	+		PFLU_RS13535	0.53	-1.5
3,065,838	+		PFLU_RS13535	0.53	+1.2
3,065,839	-		PFLU_RS13535	0.53	-0.5
3,065,839	-		PFLU_RS13535	0.53	-2.9
3,065,839	-		PFLU_RS13535	0.53	+2.3
3,065,839	-		PFLU_RS13535	0.53	-1.4
3,065,839	-		PFLU_RS13535	0.53	+1.7
3,065,839	-		PFLU_RS13535	0.53	+0.2
3,065,839	-		PFLU_RS13535	0.53	+0.7
3,065,839	-		PFLU_RS13535	0.53	+1.9
3,065,839	-		PFLU_RS13535	0.53	+1.7
3,065,987	+				-1.7
3,065,987	+				-0.6
3,065,987	+				+2.5
3,065,987	+				+1.5
3,065,987	+				+3.4
3,065,987	+				+1.2
3,065,987	+				+2.6
3,065,987	+				-0.6
3,065,987	+				+3.4
3,065,987	+				-1.4
3,065,987	+				+0.2
3,065,988	-				+0.9
3,065,988	-				-1.4
3,065,988	-				-1.3
3,065,988	-				-1.8
3,065,988	-				+3.1
3,066,053	+				-0.8
3,066,053	+				-2.5
3,066,053	+				-1.4
3,066,053	+				-1.7
3,066,053	+				-0.3
3,066,053	+				+3.0
3,066,053	+				+1.9
3,066,053	+				+0.3
3,066,053	+				+1.1
3,066,054	-				+1.5
3,066,054	-				-2.4
3,066,054	-				+1.7
3,066,054	-				-2.3
3,066,054	-				-1.1
3,066,054	-				+0.4
3,066,054	-				-0.0
3,066,054	-				+0.7
3,066,056	+				-0.3
3,066,056	+				+0.2
3,066,056	+				-0.6
3,066,056	+				+1.2
3,066,056	+				+1.0
3,066,056	+				+0.8
3,066,056	+				+1.7
3,066,056	+				-1.3
3,066,056	+				+1.7
3,066,056	+				-0.6
3,066,057	-				-1.8
3,066,057	-				-2.3
3,066,057	-				+1.2
3,066,057	-				-1.4
3,066,057	-				-0.6
3,066,057	-				+0.7
3,066,057	-				-1.8
3,066,057	-				-1.3
3,066,057	-				+1.6
3,066,057	-				-0.0
3,066,057	-				+1.5
3,066,057	-				-1.3
3,066,104	+				+0.7
3,066,104	+				-0.3
3,066,104	+				+0.7
3,066,105	-				+0.2
3,066,414	+		PFLU_RS13540	0.29	+0.2
3,066,566	+		PFLU_RS13540	0.41	-0.3
3,066,566	+		PFLU_RS13540	0.41	+0.2
3,066,566	+		PFLU_RS13540	0.41	-0.6
3,066,567	-		PFLU_RS13540	0.41	+0.2
3,066,567	-		PFLU_RS13540	0.41	+0.7
3,066,567	-		PFLU_RS13540	0.41	+1.7
3,066,567	-		PFLU_RS13540	0.41	-0.8
3,066,567	-		PFLU_RS13540	0.41	+0.7
3,066,567	-		PFLU_RS13540	0.41	+0.7
3,066,600	-		PFLU_RS13540	0.44	-0.8
3,066,787	+		PFLU_RS13540	0.58	+0.7
3,066,791	+		PFLU_RS13540	0.59	-0.6
3,066,791	+		PFLU_RS13540	0.59	+1.7
3,066,791	+		PFLU_RS13540	0.59	+0.7
3,066,792	-		PFLU_RS13540	0.59	-1.6
3,066,792	-		PFLU_RS13540	0.59	+1.7
3,066,792	-		PFLU_RS13540	0.59	-1.1
3,066,792	-		PFLU_RS13540	0.59	+1.7
3,066,792	-		PFLU_RS13540	0.59	-1.1
3,066,947	+		PFLU_RS13540	0.71	+0.7
3,066,947	+		PFLU_RS13540	0.71	-0.3
3,066,947	+		PFLU_RS13540	0.71	+0.7
3,066,947	+		PFLU_RS13540	0.71	+0.2
3,066,947	+		PFLU_RS13540	0.71	+1.7
3,066,947	+		PFLU_RS13540	0.71	+0.2
3,066,947	+		PFLU_RS13540	0.71	+0.7
3,066,947	+		PFLU_RS13540	0.71	-0.3
3,066,947	+		PFLU_RS13540	0.71	+0.2
3,066,947	+		PFLU_RS13540	0.71	+0.7
3,066,948	-		PFLU_RS13540	0.71	-0.3
3,066,948	-		PFLU_RS13540	0.71	-0.3
3,066,948	-		PFLU_RS13540	0.71	+0.2
3,066,948	-		PFLU_RS13540	0.71	+0.7
3,066,948	-		PFLU_RS13540	0.71	-0.3