Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS01860

Experiment: Growth at pH9 and (C) Trisodium citrate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS01850 and PFLU_RS01855 are separated by 37 nucleotidesPFLU_RS01855 and PFLU_RS01860 overlap by 8 nucleotidesPFLU_RS01860 and PFLU_RS01865 are separated by 193 nucleotides PFLU_RS01850: PFLU_RS01850 - transporter substrate-binding domain-containing protein, at 418,181 to 418,978 _RS01850 PFLU_RS01855: PFLU_RS01855 - amino acid ABC transporter permease, at 419,016 to 419,975 _RS01855 PFLU_RS01860: PFLU_RS01860 - amino acid ABC transporter ATP-binding protein, at 419,968 to 420,702 _RS01860 PFLU_RS01865: PFLU_RS01865 - methyl-accepting chemotaxis protein, at 420,896 to 422,803 _RS01865 Position (kb) 419 420 421Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 418.977 kb on - strandat 418.977 kb on - strandat 419.013 kb on + strandat 419.013 kb on + strandat 419.091 kb on + strandat 419.091 kb on + strandat 419.091 kb on + strandat 419.091 kb on + strandat 419.092 kb on - strandat 419.092 kb on - strandat 419.092 kb on - strandat 419.094 kb on + strandat 419.094 kb on + strandat 419.094 kb on + strandat 419.094 kb on + strandat 419.094 kb on + strandat 419.095 kb on - strandat 419.095 kb on - strandat 419.095 kb on - strandat 419.095 kb on - strandat 419.095 kb on - strandat 419.095 kb on - strandat 419.115 kb on + strand, within PFLU_RS01855at 419.115 kb on + strand, within PFLU_RS01855at 419.116 kb on - strand, within PFLU_RS01855at 419.116 kb on - strand, within PFLU_RS01855at 419.116 kb on - strand, within PFLU_RS01855at 419.145 kb on + strand, within PFLU_RS01855at 419.145 kb on + strand, within PFLU_RS01855at 419.146 kb on - strand, within PFLU_RS01855at 419.389 kb on + strand, within PFLU_RS01855at 419.389 kb on + strand, within PFLU_RS01855at 419.390 kb on - strand, within PFLU_RS01855at 419.390 kb on - strand, within PFLU_RS01855at 419.390 kb on - strand, within PFLU_RS01855at 419.390 kb on - strand, within PFLU_RS01855at 419.390 kb on - strand, within PFLU_RS01855at 419.390 kb on - strand, within PFLU_RS01855at 419.390 kb on - strand, within PFLU_RS01855at 419.390 kb on - strand, within PFLU_RS01855at 419.493 kb on + strand, within PFLU_RS01855at 419.493 kb on + strand, within PFLU_RS01855at 419.493 kb on + strand, within PFLU_RS01855at 419.493 kb on + strand, within PFLU_RS01855at 419.493 kb on + strand, within PFLU_RS01855at 419.493 kb on + strand, within PFLU_RS01855at 419.494 kb on - strand, within PFLU_RS01855at 419.494 kb on - strand, within PFLU_RS01855at 419.494 kb on - strand, within PFLU_RS01855at 419.494 kb on - strand, within PFLU_RS01855at 419.513 kb on + strand, within PFLU_RS01855at 419.521 kb on + strand, within PFLU_RS01855at 419.521 kb on + strand, within PFLU_RS01855at 419.544 kb on + strand, within PFLU_RS01855at 419.544 kb on + strand, within PFLU_RS01855at 419.545 kb on - strand, within PFLU_RS01855at 419.559 kb on - strand, within PFLU_RS01855at 419.612 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.622 kb on + strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.623 kb on - strand, within PFLU_RS01855at 419.729 kb on - strand, within PFLU_RS01855at 419.777 kb on + strand, within PFLU_RS01855at 419.777 kb on + strand, within PFLU_RS01855at 419.778 kb on - strand, within PFLU_RS01855at 419.904 kb on + strandat 419.904 kb on + strandat 419.905 kb on - strandat 419.990 kb on + strandat 419.991 kb on - strandat 419.991 kb on - strandat 420.065 kb on + strand, within PFLU_RS01860at 420.065 kb on + strand, within PFLU_RS01860at 420.065 kb on + strand, within PFLU_RS01860at 420.066 kb on - strand, within PFLU_RS01860at 420.066 kb on - strand, within PFLU_RS01860at 420.071 kb on + strand, within PFLU_RS01860at 420.072 kb on - strand, within PFLU_RS01860at 420.203 kb on - strand, within PFLU_RS01860at 420.700 kb on + strandat 420.700 kb on + strandat 420.700 kb on + strandat 420.701 kb on - strandat 420.701 kb on - strandat 420.721 kb on + strandat 420.779 kb on - strandat 421.011 kb on - strandat 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.283 kb on + strand, within PFLU_RS01865at 421.284 kb on - strand, within PFLU_RS01865at 421.284 kb on - strand, within PFLU_RS01865at 421.284 kb on - strand, within PFLU_RS01865at 421.284 kb on - strand, within PFLU_RS01865at 421.284 kb on - strand, within PFLU_RS01865at 421.295 kb on + strand, within PFLU_RS01865at 421.552 kb on + strand, within PFLU_RS01865

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth at pH9 and (C) Trisodium citrate
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418,977 - -1.6
418,977 - +3.4
419,013 + +3.7
419,013 + -1.5
419,091 + -1.8
419,091 + -0.1
419,091 + +2.2
419,091 + +1.1
419,092 - +4.7
419,092 - +2.5
419,092 - +1.5
419,094 + +2.8
419,094 + +1.5
419,094 + -0.6
419,094 + -0.1
419,094 + +0.3
419,095 - +2.1
419,095 - +1.8
419,095 - -0.2
419,095 - -1.5
419,095 - +0.5
419,095 - +2.1
419,115 + PFLU_RS01855 0.10 +3.2
419,115 + PFLU_RS01855 0.10 +0.6
419,116 - PFLU_RS01855 0.10 +1.4
419,116 - PFLU_RS01855 0.10 +1.0
419,116 - PFLU_RS01855 0.10 +4.3
419,145 + PFLU_RS01855 0.13 +1.4
419,145 + PFLU_RS01855 0.13 +0.3
419,146 - PFLU_RS01855 0.14 +1.3
419,389 + PFLU_RS01855 0.39 -2.1
419,389 + PFLU_RS01855 0.39 +1.7
419,390 - PFLU_RS01855 0.39 +0.9
419,390 - PFLU_RS01855 0.39 +1.8
419,390 - PFLU_RS01855 0.39 +2.1
419,390 - PFLU_RS01855 0.39 +2.2
419,390 - PFLU_RS01855 0.39 -1.3
419,390 - PFLU_RS01855 0.39 +0.6
419,390 - PFLU_RS01855 0.39 +2.3
419,390 - PFLU_RS01855 0.39 +1.4
419,493 + PFLU_RS01855 0.50 +0.7
419,493 + PFLU_RS01855 0.50 +1.4
419,493 + PFLU_RS01855 0.50 +2.8
419,493 + PFLU_RS01855 0.50 -1.7
419,493 + PFLU_RS01855 0.50 -0.1
419,493 + PFLU_RS01855 0.50 -2.3
419,494 - PFLU_RS01855 0.50 -0.7
419,494 - PFLU_RS01855 0.50 -0.1
419,494 - PFLU_RS01855 0.50 -1.7
419,494 - PFLU_RS01855 0.50 -1.5
419,513 + PFLU_RS01855 0.52 +0.3
419,521 + PFLU_RS01855 0.53 +1.9
419,521 + PFLU_RS01855 0.53 +1.1
419,544 + PFLU_RS01855 0.55 +0.3
419,544 + PFLU_RS01855 0.55 -0.1
419,545 - PFLU_RS01855 0.55 +1.1
419,559 - PFLU_RS01855 0.57 +1.6
419,612 + PFLU_RS01855 0.62 +0.9
419,622 + PFLU_RS01855 0.63 +2.9
419,622 + PFLU_RS01855 0.63 +2.3
419,622 + PFLU_RS01855 0.63 +0.8
419,622 + PFLU_RS01855 0.63 -0.4
419,622 + PFLU_RS01855 0.63 +2.1
419,622 + PFLU_RS01855 0.63 +0.9
419,622 + PFLU_RS01855 0.63 +4.0
419,622 + PFLU_RS01855 0.63 -0.3
419,622 + PFLU_RS01855 0.63 -0.6
419,622 + PFLU_RS01855 0.63 +4.0
419,622 + PFLU_RS01855 0.63 -1.3
419,622 + PFLU_RS01855 0.63 -1.9
419,622 + PFLU_RS01855 0.63 -0.4
419,622 + PFLU_RS01855 0.63 +0.9
419,623 - PFLU_RS01855 0.63 -1.0
419,623 - PFLU_RS01855 0.63 +2.9
419,623 - PFLU_RS01855 0.63 +1.0
419,623 - PFLU_RS01855 0.63 +0.3
419,623 - PFLU_RS01855 0.63 +3.6
419,623 - PFLU_RS01855 0.63 +1.3
419,623 - PFLU_RS01855 0.63 -0.6
419,623 - PFLU_RS01855 0.63 +3.2
419,623 - PFLU_RS01855 0.63 +0.4
419,623 - PFLU_RS01855 0.63 -0.1
419,623 - PFLU_RS01855 0.63 -1.5
419,623 - PFLU_RS01855 0.63 -0.3
419,623 - PFLU_RS01855 0.63 +1.4
419,729 - PFLU_RS01855 0.74 +2.1
419,777 + PFLU_RS01855 0.79 -1.4
419,777 + PFLU_RS01855 0.79 -1.7
419,778 - PFLU_RS01855 0.79 +1.9
419,904 + -1.4
419,904 + -1.7
419,905 - +1.9
419,990 + +2.4
419,991 - +1.8
419,991 - +4.1
420,065 + PFLU_RS01860 0.13 -1.4
420,065 + PFLU_RS01860 0.13 +0.8
420,065 + PFLU_RS01860 0.13 -1.1
420,066 - PFLU_RS01860 0.13 -1.9
420,066 - PFLU_RS01860 0.13 +2.3
420,071 + PFLU_RS01860 0.14 +1.5
420,072 - PFLU_RS01860 0.14 +1.8
420,203 - PFLU_RS01860 0.32 +2.6
420,700 + +1.5
420,700 + -1.3
420,700 + -1.5
420,701 - +0.3
420,701 - -0.3
420,721 + +1.4
420,779 - -0.2
421,011 - +0.1
421,283 + PFLU_RS01865 0.20 -0.4
421,283 + PFLU_RS01865 0.20 +0.5
421,283 + PFLU_RS01865 0.20 -2.4
421,283 + PFLU_RS01865 0.20 +1.3
421,283 + PFLU_RS01865 0.20 -2.1
421,283 + PFLU_RS01865 0.20 +4.8
421,283 + PFLU_RS01865 0.20 -2.3
421,283 + PFLU_RS01865 0.20 -2.6
421,283 + PFLU_RS01865 0.20 +2.2
421,283 + PFLU_RS01865 0.20 -0.1
421,283 + PFLU_RS01865 0.20 +2.0
421,283 + PFLU_RS01865 0.20 +4.7
421,283 + PFLU_RS01865 0.20 +2.3
421,284 - PFLU_RS01865 0.20 -0.6
421,284 - PFLU_RS01865 0.20 +0.1
421,284 - PFLU_RS01865 0.20 +1.9
421,284 - PFLU_RS01865 0.20 +2.9
421,284 - PFLU_RS01865 0.20 +2.4
421,295 + PFLU_RS01865 0.21 +1.1
421,552 + PFLU_RS01865 0.34 -0.6

Or see this region's nucleotide sequence