Experiment: PYE with Spectinomycin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02343 and CCNA_02344 are separated by 92 nucleotides CCNA_02344 and CCNA_02345 are separated by 160 nucleotides CCNA_02345 and CCNA_02346 overlap by 4 nucleotides
CCNA_02343: CCNA_02343 - hypothetical protein with pentapeptide repeats, at 2,482,784 to 2,484,043
_02343
CCNA_02344: CCNA_02344 - phosphoglycerate mutase, at 2,484,136 to 2,484,849
_02344
CCNA_02345: CCNA_02345 - hypothetical protein, at 2,485,010 to 2,485,225
_02345
CCNA_02346: CCNA_02346 - arabinose-5-phosphate isomerase, at 2,485,222 to 2,486,178
_02346
Position (kb)
2485
2486 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2484.082 kb on - strand at 2484.141 kb on - strand at 2484.153 kb on - strand at 2484.179 kb on - strand at 2484.894 kb on - strand at 2484.894 kb on - strand at 2484.894 kb on - strand at 2484.900 kb on - strand at 2484.922 kb on - strand at 2484.963 kb on - strand at 2484.967 kb on - strand at 2484.967 kb on - strand at 2484.968 kb on - strand at 2484.977 kb on + strand at 2485.053 kb on + strand, within CCNA_02345 at 2485.064 kb on + strand, within CCNA_02345 at 2485.069 kb on + strand, within CCNA_02345 at 2485.069 kb on + strand, within CCNA_02345 at 2485.069 kb on + strand, within CCNA_02345 at 2485.077 kb on - strand, within CCNA_02345 at 2485.077 kb on - strand, within CCNA_02345 at 2485.084 kb on + strand, within CCNA_02345 at 2485.143 kb on - strand, within CCNA_02345 at 2485.143 kb on - strand, within CCNA_02345 at 2485.143 kb on - strand, within CCNA_02345 at 2485.143 kb on - strand, within CCNA_02345 at 2485.170 kb on + strand, within CCNA_02345 at 2485.174 kb on + strand, within CCNA_02345 at 2485.196 kb on + strand, within CCNA_02345 at 2485.230 kb on + strand at 2485.230 kb on + strand at 2485.238 kb on - strand at 2485.238 kb on - strand at 2486.191 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml remove 2,484,082 - -0.4 2,484,141 - -0.2 2,484,153 - +1.0 2,484,179 - +0.9 2,484,894 - +0.7 2,484,894 - +0.4 2,484,894 - -0.6 2,484,900 - -0.5 2,484,922 - +0.8 2,484,963 - -0.0 2,484,967 - -1.1 2,484,967 - +0.3 2,484,968 - -0.1 2,484,977 + +2.1 2,485,053 + CCNA_02345 0.20 +1.3 2,485,064 + CCNA_02345 0.25 +0.9 2,485,069 + CCNA_02345 0.27 -1.7 2,485,069 + CCNA_02345 0.27 -0.2 2,485,069 + CCNA_02345 0.27 -1.2 2,485,077 - CCNA_02345 0.31 -1.2 2,485,077 - CCNA_02345 0.31 -2.2 2,485,084 + CCNA_02345 0.34 +1.1 2,485,143 - CCNA_02345 0.62 -0.7 2,485,143 - CCNA_02345 0.62 +2.5 2,485,143 - CCNA_02345 0.62 -0.8 2,485,143 - CCNA_02345 0.62 -1.0 2,485,170 + CCNA_02345 0.74 -2.1 2,485,174 + CCNA_02345 0.76 -0.4 2,485,196 + CCNA_02345 0.86 +0.5 2,485,230 + +0.4 2,485,230 + +1.0 2,485,238 - -0.8 2,485,238 - -0.7 2,486,191 - -0.6
Or see this region's nucleotide sequence