Experiment: PYE with Spectinomycin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01946 and CCNA_01947 are separated by 127 nucleotides CCNA_01947 and CCNA_01948 are separated by 233 nucleotides
CCNA_01946: CCNA_01946 - tyrosyl-tRNA synthetase, at 2,088,215 to 2,089,540
_01946
CCNA_01947: CCNA_01947 - thioredoxin peroxidase, at 2,089,668 to 2,090,129
_01947
CCNA_01948: CCNA_01948 - peptidoglycan-specific endopeptidase, M23 family, at 2,090,363 to 2,091,514
_01948
Position (kb)
2089
2090
2091 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 2089.525 kb on + strand at 2089.588 kb on + strand at 2089.596 kb on - strand at 2089.596 kb on - strand at 2089.596 kb on - strand at 2089.596 kb on - strand at 2089.596 kb on - strand at 2089.633 kb on - strand at 2089.635 kb on + strand at 2089.661 kb on + strand at 2089.798 kb on + strand, within CCNA_01947 at 2089.798 kb on + strand, within CCNA_01947 at 2089.806 kb on - strand, within CCNA_01947 at 2089.817 kb on - strand, within CCNA_01947 at 2089.917 kb on - strand, within CCNA_01947 at 2090.011 kb on - strand, within CCNA_01947 at 2090.100 kb on + strand at 2090.100 kb on + strand at 2090.110 kb on + strand at 2090.145 kb on + strand at 2090.208 kb on + strand at 2090.208 kb on + strand at 2090.208 kb on + strand at 2090.208 kb on + strand at 2090.216 kb on - strand at 2090.216 kb on - strand at 2090.243 kb on + strand at 2090.280 kb on + strand at 2090.425 kb on + strand at 2090.455 kb on + strand at 2090.465 kb on - strand at 2090.485 kb on + strand, within CCNA_01948 at 2090.526 kb on - strand, within CCNA_01948 at 2090.526 kb on - strand, within CCNA_01948 at 2090.612 kb on + strand, within CCNA_01948 at 2090.627 kb on + strand, within CCNA_01948 at 2090.635 kb on - strand, within CCNA_01948 at 2090.636 kb on + strand, within CCNA_01948 at 2090.636 kb on + strand, within CCNA_01948 at 2090.636 kb on + strand, within CCNA_01948 at 2090.640 kb on - strand, within CCNA_01948 at 2090.644 kb on - strand, within CCNA_01948 at 2090.644 kb on - strand, within CCNA_01948 at 2090.644 kb on - strand, within CCNA_01948 at 2090.727 kb on + strand, within CCNA_01948 at 2090.727 kb on + strand, within CCNA_01948 at 2090.735 kb on - strand, within CCNA_01948 at 2090.737 kb on - strand, within CCNA_01948 at 2090.840 kb on + strand, within CCNA_01948 at 2090.848 kb on - strand, within CCNA_01948 at 2090.849 kb on + strand, within CCNA_01948 at 2090.864 kb on + strand, within CCNA_01948 at 2090.864 kb on + strand, within CCNA_01948 at 2090.864 kb on + strand, within CCNA_01948 at 2090.872 kb on - strand, within CCNA_01948 at 2090.872 kb on - strand, within CCNA_01948 at 2090.921 kb on + strand, within CCNA_01948 at 2090.986 kb on + strand, within CCNA_01948 at 2091.016 kb on - strand, within CCNA_01948 at 2091.016 kb on - strand, within CCNA_01948 at 2091.028 kb on - strand, within CCNA_01948
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml remove 2,089,525 + +0.4 2,089,588 + +1.0 2,089,596 - -0.3 2,089,596 - -0.3 2,089,596 - +0.9 2,089,596 - -0.9 2,089,596 - +1.0 2,089,633 - -0.4 2,089,635 + -0.2 2,089,661 + +0.5 2,089,798 + CCNA_01947 0.28 -2.1 2,089,798 + CCNA_01947 0.28 -1.1 2,089,806 - CCNA_01947 0.30 +0.9 2,089,817 - CCNA_01947 0.32 -0.9 2,089,917 - CCNA_01947 0.54 -0.4 2,090,011 - CCNA_01947 0.74 -1.0 2,090,100 + +2.6 2,090,100 + +0.1 2,090,110 + +0.6 2,090,145 + +0.7 2,090,208 + +0.7 2,090,208 + +1.1 2,090,208 + +1.9 2,090,208 + +1.1 2,090,216 - -0.4 2,090,216 - -1.1 2,090,243 + +0.4 2,090,280 + +8.5 2,090,425 + +0.1 2,090,455 + +0.3 2,090,465 - +1.4 2,090,485 + CCNA_01948 0.11 +1.1 2,090,526 - CCNA_01948 0.14 -0.2 2,090,526 - CCNA_01948 0.14 -1.6 2,090,612 + CCNA_01948 0.22 -0.4 2,090,627 + CCNA_01948 0.23 -1.3 2,090,635 - CCNA_01948 0.24 +0.1 2,090,636 + CCNA_01948 0.24 -1.0 2,090,636 + CCNA_01948 0.24 +1.0 2,090,636 + CCNA_01948 0.24 +0.3 2,090,640 - CCNA_01948 0.24 -1.3 2,090,644 - CCNA_01948 0.24 -1.0 2,090,644 - CCNA_01948 0.24 +0.5 2,090,644 - CCNA_01948 0.24 -2.9 2,090,727 + CCNA_01948 0.32 -0.5 2,090,727 + CCNA_01948 0.32 -1.5 2,090,735 - CCNA_01948 0.32 -0.2 2,090,737 - CCNA_01948 0.32 +0.3 2,090,840 + CCNA_01948 0.41 +0.7 2,090,848 - CCNA_01948 0.42 -0.1 2,090,849 + CCNA_01948 0.42 +0.5 2,090,864 + CCNA_01948 0.43 +0.3 2,090,864 + CCNA_01948 0.43 +0.0 2,090,864 + CCNA_01948 0.43 +0.5 2,090,872 - CCNA_01948 0.44 +1.4 2,090,872 - CCNA_01948 0.44 -1.0 2,090,921 + CCNA_01948 0.48 -0.1 2,090,986 + CCNA_01948 0.54 -0.2 2,091,016 - CCNA_01948 0.57 -0.8 2,091,016 - CCNA_01948 0.57 -0.6 2,091,028 - CCNA_01948 0.58 +1.9
Or see this region's nucleotide sequence