Experiment: PYE with Spectinomycin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01750 and CCNA_01751 overlap by 4 nucleotides CCNA_01751 and CCNA_01752 are separated by 3 nucleotides CCNA_01752 and CCNA_01753 overlap by 8 nucleotides
CCNA_01750: CCNA_01750 - 3-oxoacyl-(acyl-carrier-protein) synthase II, at 1,877,432 to 1,878,712
_01750
CCNA_01751: CCNA_01751 - aminodeoxychorismate lyase, at 1,878,709 to 1,879,809
_01751
CCNA_01752: CCNA_01752 - YicC-family protein, at 1,879,813 to 1,880,694
_01752
CCNA_01753: CCNA_01753 - guanylate kinase, at 1,880,687 to 1,881,328
_01753
Position (kb)
1878
1879
1880 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1878.849 kb on + strand, within CCNA_01751 at 1878.921 kb on + strand, within CCNA_01751 at 1878.956 kb on + strand, within CCNA_01751 at 1879.209 kb on + strand, within CCNA_01751 at 1879.235 kb on + strand, within CCNA_01751 at 1879.243 kb on + strand, within CCNA_01751 at 1879.243 kb on + strand, within CCNA_01751 at 1879.557 kb on + strand, within CCNA_01751 at 1879.565 kb on - strand, within CCNA_01751 at 1879.565 kb on - strand, within CCNA_01751 at 1879.649 kb on + strand, within CCNA_01751 at 1879.713 kb on - strand at 1879.824 kb on + strand at 1879.853 kb on - strand at 1879.983 kb on + strand, within CCNA_01752 at 1879.991 kb on - strand, within CCNA_01752 at 1879.991 kb on - strand, within CCNA_01752 at 1879.991 kb on - strand, within CCNA_01752 at 1880.062 kb on + strand, within CCNA_01752 at 1880.121 kb on + strand, within CCNA_01752 at 1880.153 kb on - strand, within CCNA_01752 at 1880.153 kb on - strand, within CCNA_01752 at 1880.202 kb on + strand, within CCNA_01752 at 1880.334 kb on + strand, within CCNA_01752 at 1880.340 kb on + strand, within CCNA_01752 at 1880.342 kb on - strand, within CCNA_01752 at 1880.342 kb on - strand, within CCNA_01752 at 1880.376 kb on + strand, within CCNA_01752 at 1880.393 kb on + strand, within CCNA_01752 at 1880.393 kb on + strand, within CCNA_01752 at 1880.403 kb on + strand, within CCNA_01752 at 1880.479 kb on + strand, within CCNA_01752 at 1880.479 kb on + strand, within CCNA_01752 at 1880.487 kb on - strand, within CCNA_01752 at 1880.523 kb on + strand, within CCNA_01752 at 1880.523 kb on + strand, within CCNA_01752 at 1880.567 kb on + strand, within CCNA_01752 at 1880.586 kb on + strand, within CCNA_01752 at 1880.623 kb on - strand at 1880.623 kb on - strand at 1880.636 kb on + strand at 1880.644 kb on - strand at 1880.651 kb on - strand at 1880.659 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml remove 1,878,849 + CCNA_01751 0.13 +3.5 1,878,921 + CCNA_01751 0.19 +3.9 1,878,956 + CCNA_01751 0.22 +3.7 1,879,209 + CCNA_01751 0.45 +3.9 1,879,235 + CCNA_01751 0.48 +4.8 1,879,243 + CCNA_01751 0.49 +1.7 1,879,243 + CCNA_01751 0.49 +4.1 1,879,557 + CCNA_01751 0.77 +3.7 1,879,565 - CCNA_01751 0.78 +1.0 1,879,565 - CCNA_01751 0.78 +3.0 1,879,649 + CCNA_01751 0.85 +3.9 1,879,713 - +4.5 1,879,824 + +0.4 1,879,853 - +0.4 1,879,983 + CCNA_01752 0.19 +0.7 1,879,991 - CCNA_01752 0.20 +1.4 1,879,991 - CCNA_01752 0.20 -0.6 1,879,991 - CCNA_01752 0.20 -0.4 1,880,062 + CCNA_01752 0.28 +1.1 1,880,121 + CCNA_01752 0.35 +1.8 1,880,153 - CCNA_01752 0.39 +0.4 1,880,153 - CCNA_01752 0.39 +1.5 1,880,202 + CCNA_01752 0.44 +1.5 1,880,334 + CCNA_01752 0.59 -0.5 1,880,340 + CCNA_01752 0.60 +0.6 1,880,342 - CCNA_01752 0.60 -0.8 1,880,342 - CCNA_01752 0.60 +0.2 1,880,376 + CCNA_01752 0.64 +0.8 1,880,393 + CCNA_01752 0.66 -0.1 1,880,393 + CCNA_01752 0.66 +1.0 1,880,403 + CCNA_01752 0.67 +0.2 1,880,479 + CCNA_01752 0.76 +0.5 1,880,479 + CCNA_01752 0.76 +0.8 1,880,487 - CCNA_01752 0.76 +0.9 1,880,523 + CCNA_01752 0.80 +0.6 1,880,523 + CCNA_01752 0.80 +0.7 1,880,567 + CCNA_01752 0.85 +0.3 1,880,586 + CCNA_01752 0.88 +0.3 1,880,623 - -2.2 1,880,623 - +3.0 1,880,636 + +1.2 1,880,644 - +0.4 1,880,651 - -0.5 1,880,659 - +1.2
Or see this region's nucleotide sequence