Experiment: PYE with Spectinomycin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00724 and CCNA_00725 are separated by 200 nucleotides CCNA_00725 and CCNA_00726 are separated by 50 nucleotides CCNA_00726 and CCNA_00727 are separated by 156 nucleotides CCNA_00727 and CCNA_00728 are separated by 72 nucleotides
CCNA_00724: CCNA_00724 - hypothetical protein, at 780,321 to 780,749
_00724
CCNA_00725: CCNA_00725 - O6-methylguanine-DNA methyltransferase, at 780,950 to 781,612
_00725
CCNA_00726: CCNA_00726 - acyl-CoA dehydrogenase, at 781,663 to 782,970
_00726
CCNA_00727: CCNA_00727 - ribosomal-protein-alanine acetyltransferase, at 783,127 to 783,723
_00727
CCNA_00728: CCNA_00728 - hypothetical protein, at 783,796 to 784,959
_00728
Position (kb) 781
782
783 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 780.670 kb on - strand, within CCNA_00724 at 780.677 kb on - strand, within CCNA_00724 at 780.682 kb on - strand, within CCNA_00724 at 780.703 kb on - strand, within CCNA_00724 at 780.737 kb on + strand at 780.738 kb on + strand at 780.743 kb on + strand at 780.743 kb on + strand at 780.751 kb on - strand at 780.751 kb on - strand at 780.860 kb on + strand at 780.882 kb on + strand at 780.948 kb on + strand at 780.956 kb on - strand at 780.989 kb on + strand at 781.079 kb on - strand, within CCNA_00725 at 781.124 kb on + strand, within CCNA_00725 at 781.129 kb on + strand, within CCNA_00725 at 781.129 kb on + strand, within CCNA_00725 at 781.137 kb on - strand, within CCNA_00725 at 781.137 kb on - strand, within CCNA_00725 at 781.137 kb on - strand, within CCNA_00725 at 781.148 kb on - strand, within CCNA_00725 at 781.166 kb on + strand, within CCNA_00725 at 781.174 kb on - strand, within CCNA_00725 at 781.220 kb on - strand, within CCNA_00725 at 781.220 kb on - strand, within CCNA_00725 at 781.225 kb on - strand, within CCNA_00725 at 781.317 kb on - strand, within CCNA_00725 at 781.356 kb on - strand, within CCNA_00725 at 781.394 kb on - strand, within CCNA_00725 at 781.409 kb on - strand, within CCNA_00725 at 781.418 kb on - strand, within CCNA_00725 at 781.450 kb on - strand, within CCNA_00725 at 781.481 kb on - strand, within CCNA_00725 at 781.513 kb on + strand, within CCNA_00725 at 781.613 kb on - strand at 781.689 kb on - strand at 781.689 kb on - strand at 781.717 kb on + strand at 781.718 kb on - strand at 781.736 kb on + strand at 781.762 kb on - strand at 781.770 kb on - strand at 781.772 kb on - strand at 781.783 kb on - strand at 781.797 kb on + strand, within CCNA_00726 at 781.817 kb on + strand, within CCNA_00726 at 781.817 kb on + strand, within CCNA_00726 at 781.825 kb on - strand, within CCNA_00726 at 781.825 kb on - strand, within CCNA_00726 at 781.825 kb on - strand, within CCNA_00726 at 781.827 kb on + strand, within CCNA_00726 at 781.827 kb on + strand, within CCNA_00726 at 781.835 kb on - strand, within CCNA_00726 at 781.835 kb on - strand, within CCNA_00726 at 781.835 kb on - strand, within CCNA_00726 at 781.848 kb on - strand, within CCNA_00726 at 781.861 kb on + strand, within CCNA_00726 at 781.897 kb on + strand, within CCNA_00726 at 781.910 kb on + strand, within CCNA_00726 at 781.918 kb on - strand, within CCNA_00726 at 781.923 kb on - strand, within CCNA_00726 at 781.934 kb on + strand, within CCNA_00726 at 781.981 kb on - strand, within CCNA_00726 at 782.022 kb on - strand, within CCNA_00726 at 782.036 kb on - strand, within CCNA_00726 at 782.040 kb on - strand, within CCNA_00726 at 782.063 kb on + strand, within CCNA_00726 at 782.070 kb on + strand, within CCNA_00726 at 782.164 kb on - strand, within CCNA_00726 at 782.175 kb on - strand, within CCNA_00726 at 782.175 kb on - strand, within CCNA_00726 at 782.186 kb on - strand, within CCNA_00726 at 782.210 kb on - strand, within CCNA_00726 at 782.234 kb on + strand, within CCNA_00726 at 782.254 kb on - strand, within CCNA_00726 at 782.260 kb on - strand, within CCNA_00726 at 782.265 kb on - strand, within CCNA_00726 at 782.279 kb on - strand at 782.279 kb on - strand, within CCNA_00726 at 782.349 kb on - strand, within CCNA_00726 at 782.386 kb on - strand, within CCNA_00726 at 782.421 kb on + strand, within CCNA_00726 at 782.429 kb on - strand, within CCNA_00726 at 782.429 kb on - strand, within CCNA_00726 at 782.483 kb on - strand, within CCNA_00726 at 782.490 kb on - strand, within CCNA_00726 at 782.490 kb on - strand, within CCNA_00726 at 782.507 kb on - strand, within CCNA_00726 at 782.507 kb on - strand, within CCNA_00726 at 782.512 kb on - strand, within CCNA_00726 at 782.517 kb on - strand, within CCNA_00726 at 782.525 kb on + strand, within CCNA_00726 at 782.613 kb on - strand, within CCNA_00726 at 782.650 kb on - strand, within CCNA_00726 at 782.702 kb on - strand, within CCNA_00726 at 782.723 kb on + strand, within CCNA_00726 at 782.723 kb on + strand, within CCNA_00726 at 782.724 kb on - strand, within CCNA_00726 at 782.731 kb on - strand, within CCNA_00726 at 782.746 kb on - strand, within CCNA_00726 at 782.752 kb on + strand, within CCNA_00726 at 782.755 kb on - strand, within CCNA_00726 at 782.760 kb on - strand, within CCNA_00726 at 782.760 kb on - strand, within CCNA_00726 at 782.760 kb on - strand, within CCNA_00726 at 782.760 kb on - strand, within CCNA_00726 at 782.760 kb on - strand, within CCNA_00726 at 782.760 kb on - strand, within CCNA_00726 at 782.838 kb on - strand, within CCNA_00726 at 782.881 kb on - strand at 782.926 kb on - strand at 782.927 kb on - strand at 782.929 kb on + strand at 782.954 kb on - strand at 782.959 kb on + strand at 782.965 kb on + strand at 782.965 kb on + strand at 782.965 kb on + strand at 782.965 kb on + strand at 782.965 kb on + strand at 782.973 kb on - strand at 782.973 kb on - strand at 782.973 kb on - strand at 782.973 kb on - strand at 783.028 kb on - strand at 783.038 kb on - strand at 783.067 kb on + strand at 783.067 kb on + strand at 783.075 kb on - strand at 783.075 kb on - strand at 783.075 kb on - strand at 783.075 kb on - strand at 783.075 kb on - strand at 783.075 kb on - strand at 783.080 kb on - strand at 783.084 kb on + strand at 783.092 kb on - strand at 783.092 kb on - strand at 783.128 kb on + strand at 783.130 kb on - strand at 783.154 kb on - strand at 783.214 kb on + strand, within CCNA_00727 at 783.236 kb on + strand, within CCNA_00727 at 783.272 kb on + strand, within CCNA_00727 at 783.282 kb on + strand, within CCNA_00727 at 783.290 kb on - strand, within CCNA_00727 at 783.295 kb on + strand, within CCNA_00727 at 783.300 kb on + strand, within CCNA_00727 at 783.300 kb on + strand, within CCNA_00727 at 783.303 kb on - strand, within CCNA_00727 at 783.387 kb on - strand, within CCNA_00727 at 783.387 kb on - strand, within CCNA_00727 at 783.429 kb on + strand, within CCNA_00727 at 783.432 kb on - strand, within CCNA_00727 at 783.442 kb on - strand, within CCNA_00727 at 783.446 kb on + strand, within CCNA_00727 at 783.451 kb on + strand, within CCNA_00727 at 783.455 kb on - strand, within CCNA_00727 at 783.459 kb on - strand, within CCNA_00727 at 783.462 kb on + strand, within CCNA_00727 at 783.464 kb on - strand, within CCNA_00727 at 783.504 kb on + strand, within CCNA_00727 at 783.504 kb on + strand, within CCNA_00727 at 783.504 kb on + strand, within CCNA_00727 at 783.523 kb on + strand, within CCNA_00727 at 783.523 kb on + strand, within CCNA_00727 at 783.528 kb on + strand, within CCNA_00727 at 783.528 kb on + strand, within CCNA_00727 at 783.605 kb on + strand, within CCNA_00727 at 783.605 kb on + strand, within CCNA_00727 at 783.639 kb on - strand, within CCNA_00727 at 783.656 kb on - strand, within CCNA_00727 at 783.705 kb on + strand at 783.736 kb on - strand at 783.737 kb on - strand at 783.775 kb on + strand at 783.783 kb on - strand at 783.783 kb on - strand at 783.802 kb on + strand at 783.826 kb on + strand at 783.845 kb on + strand at 783.924 kb on + strand, within CCNA_00728 at 783.953 kb on + strand, within CCNA_00728 at 783.953 kb on + strand, within CCNA_00728
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml remove 780,670 - CCNA_00724 0.81 +0.6 780,677 - CCNA_00724 0.83 -0.7 780,682 - CCNA_00724 0.84 +1.5 780,703 - CCNA_00724 0.89 -0.6 780,737 + +0.4 780,738 + +0.2 780,743 + +0.2 780,743 + +1.1 780,751 - -1.4 780,751 - +3.9 780,860 + -0.3 780,882 + -0.2 780,948 + +0.9 780,956 - -0.7 780,989 + +1.0 781,079 - CCNA_00725 0.19 -1.3 781,124 + CCNA_00725 0.26 -0.4 781,129 + CCNA_00725 0.27 -0.9 781,129 + CCNA_00725 0.27 -0.0 781,137 - CCNA_00725 0.28 +0.5 781,137 - CCNA_00725 0.28 +0.3 781,137 - CCNA_00725 0.28 -1.3 781,148 - CCNA_00725 0.30 +1.0 781,166 + CCNA_00725 0.33 +0.6 781,174 - CCNA_00725 0.34 +0.5 781,220 - CCNA_00725 0.41 +0.9 781,220 - CCNA_00725 0.41 +1.0 781,225 - CCNA_00725 0.41 +1.2 781,317 - CCNA_00725 0.55 -0.5 781,356 - CCNA_00725 0.61 +1.0 781,394 - CCNA_00725 0.67 -1.6 781,409 - CCNA_00725 0.69 -0.7 781,418 - CCNA_00725 0.71 -0.4 781,450 - CCNA_00725 0.75 -0.4 781,481 - CCNA_00725 0.80 +0.6 781,513 + CCNA_00725 0.85 +0.7 781,613 - -1.4 781,689 - +0.6 781,689 - -0.3 781,717 + -0.5 781,718 - -2.0 781,736 + -0.2 781,762 - +0.8 781,770 - +1.1 781,772 - +1.4 781,783 - +0.6 781,797 + CCNA_00726 0.10 +0.8 781,817 + CCNA_00726 0.12 -0.2 781,817 + CCNA_00726 0.12 -0.7 781,825 - CCNA_00726 0.12 +0.7 781,825 - CCNA_00726 0.12 +0.7 781,825 - CCNA_00726 0.12 +1.8 781,827 + CCNA_00726 0.13 -0.9 781,827 + CCNA_00726 0.13 +0.7 781,835 - CCNA_00726 0.13 +0.9 781,835 - CCNA_00726 0.13 +0.6 781,835 - CCNA_00726 0.13 +0.8 781,848 - CCNA_00726 0.14 +1.0 781,861 + CCNA_00726 0.15 +1.4 781,897 + CCNA_00726 0.18 -0.1 781,910 + CCNA_00726 0.19 -0.7 781,918 - CCNA_00726 0.19 +2.5 781,923 - CCNA_00726 0.20 +0.0 781,934 + CCNA_00726 0.21 +1.2 781,981 - CCNA_00726 0.24 +1.4 782,022 - CCNA_00726 0.27 +1.2 782,036 - CCNA_00726 0.29 +1.3 782,040 - CCNA_00726 0.29 +0.8 782,063 + CCNA_00726 0.31 +1.3 782,070 + CCNA_00726 0.31 -0.6 782,164 - CCNA_00726 0.38 +0.5 782,175 - CCNA_00726 0.39 -0.4 782,175 - CCNA_00726 0.39 +0.5 782,186 - CCNA_00726 0.40 +0.6 782,210 - CCNA_00726 0.42 -0.1 782,234 + CCNA_00726 0.44 +2.0 782,254 - CCNA_00726 0.45 +1.5 782,260 - CCNA_00726 0.46 +0.3 782,265 - CCNA_00726 0.46 +1.8 782,279 - +2.4 782,279 - CCNA_00726 0.47 +0.1 782,349 - CCNA_00726 0.52 +1.1 782,386 - CCNA_00726 0.55 -0.0 782,421 + CCNA_00726 0.58 +0.1 782,429 - CCNA_00726 0.59 +0.2 782,429 - CCNA_00726 0.59 +0.3 782,483 - CCNA_00726 0.63 +0.3 782,490 - CCNA_00726 0.63 +1.2 782,490 - CCNA_00726 0.63 +1.6 782,507 - CCNA_00726 0.65 -0.5 782,507 - CCNA_00726 0.65 -0.0 782,512 - CCNA_00726 0.65 -0.1 782,517 - CCNA_00726 0.65 +0.4 782,525 + CCNA_00726 0.66 +2.0 782,613 - CCNA_00726 0.73 +0.8 782,650 - CCNA_00726 0.75 +2.2 782,702 - CCNA_00726 0.79 +2.9 782,723 + CCNA_00726 0.81 +0.6 782,723 + CCNA_00726 0.81 -1.2 782,724 - CCNA_00726 0.81 +1.3 782,731 - CCNA_00726 0.82 +0.0 782,746 - CCNA_00726 0.83 +0.8 782,752 + CCNA_00726 0.83 +0.4 782,755 - CCNA_00726 0.83 +2.2 782,760 - CCNA_00726 0.84 +0.8 782,760 - CCNA_00726 0.84 -0.5 782,760 - CCNA_00726 0.84 -0.0 782,760 - CCNA_00726 0.84 +0.5 782,760 - CCNA_00726 0.84 +0.8 782,760 - CCNA_00726 0.84 +1.7 782,838 - CCNA_00726 0.90 +0.3 782,881 - +0.7 782,926 - +1.1 782,927 - +0.6 782,929 + -0.2 782,954 - +0.2 782,959 + +0.4 782,965 + -1.2 782,965 + +1.4 782,965 + +0.6 782,965 + +0.9 782,965 + +0.8 782,973 - -1.3 782,973 - -0.6 782,973 - -0.5 782,973 - +0.1 783,028 - +1.0 783,038 - +0.7 783,067 + -0.2 783,067 + -1.3 783,075 - -0.4 783,075 - -0.4 783,075 - -0.4 783,075 - -0.2 783,075 - -0.8 783,075 - -0.3 783,080 - +1.0 783,084 + +0.1 783,092 - +2.0 783,092 - +0.9 783,128 + +1.1 783,130 - +1.6 783,154 - -1.4 783,214 + CCNA_00727 0.15 -1.0 783,236 + CCNA_00727 0.18 -0.3 783,272 + CCNA_00727 0.24 -0.7 783,282 + CCNA_00727 0.26 +2.0 783,290 - CCNA_00727 0.27 +0.8 783,295 + CCNA_00727 0.28 -1.1 783,300 + CCNA_00727 0.29 -1.4 783,300 + CCNA_00727 0.29 -2.1 783,303 - CCNA_00727 0.29 -0.4 783,387 - CCNA_00727 0.44 +1.6 783,387 - CCNA_00727 0.44 -0.9 783,429 + CCNA_00727 0.51 +1.5 783,432 - CCNA_00727 0.51 +0.7 783,442 - CCNA_00727 0.53 -0.5 783,446 + CCNA_00727 0.53 -1.2 783,451 + CCNA_00727 0.54 -0.6 783,455 - CCNA_00727 0.55 +0.1 783,459 - CCNA_00727 0.56 +1.8 783,462 + CCNA_00727 0.56 +0.3 783,464 - CCNA_00727 0.56 -1.4 783,504 + CCNA_00727 0.63 -0.1 783,504 + CCNA_00727 0.63 +0.2 783,504 + CCNA_00727 0.63 +2.4 783,523 + CCNA_00727 0.66 +0.4 783,523 + CCNA_00727 0.66 +0.3 783,528 + CCNA_00727 0.67 +0.4 783,528 + CCNA_00727 0.67 +0.2 783,605 + CCNA_00727 0.80 -0.4 783,605 + CCNA_00727 0.80 +0.3 783,639 - CCNA_00727 0.86 -0.7 783,656 - CCNA_00727 0.89 +1.2 783,705 + +2.2 783,736 - +1.0 783,737 - +1.8 783,775 + -1.4 783,783 - -0.5 783,783 - +0.8 783,802 + -1.7 783,826 + +0.4 783,845 + +0.3 783,924 + CCNA_00728 0.11 -0.5 783,953 + CCNA_00728 0.13 -1.0 783,953 + CCNA_00728 0.13 -0.1
Or see this region's nucleotide sequence