Experiment: PYE with Spectinomycin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00534 and CCNA_00535 are separated by 42 nucleotides CCNA_00535 and CCNA_00536 are separated by 293 nucleotides CCNA_00536 and CCNA_00537 are separated by 113 nucleotides
CCNA_00534: CCNA_00534 - hypothetical protein, at 546,314 to 547,336
_00534
CCNA_00535: CCNA_00535 - hypothetical protein, at 547,379 to 547,537
_00535
CCNA_00536: CCNA_00536 - DNA-directed RNA polymerase beta chain, at 547,831 to 551,901
_00536
CCNA_00537: CCNA_00537 - DNA-directed RNA polymerase beta, at 552,015 to 556,205
_00537
Position (kb) 547
548
549
550
551
552 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 546.904 kb on + strand, within CCNA_00534 at 546.928 kb on - strand, within CCNA_00534 at 546.931 kb on + strand, within CCNA_00534 at 546.931 kb on + strand, within CCNA_00534 at 546.935 kb on + strand, within CCNA_00534 at 546.939 kb on - strand, within CCNA_00534 at 546.939 kb on - strand, within CCNA_00534 at 546.939 kb on - strand, within CCNA_00534 at 546.950 kb on + strand, within CCNA_00534 at 547.055 kb on + strand, within CCNA_00534 at 547.066 kb on - strand, within CCNA_00534 at 547.082 kb on + strand, within CCNA_00534 at 547.082 kb on + strand, within CCNA_00534 at 547.084 kb on + strand, within CCNA_00534 at 547.090 kb on - strand, within CCNA_00534 at 547.096 kb on - strand, within CCNA_00534 at 547.151 kb on - strand, within CCNA_00534 at 547.181 kb on + strand, within CCNA_00534 at 547.181 kb on + strand, within CCNA_00534 at 547.216 kb on - strand, within CCNA_00534 at 547.240 kb on + strand at 547.240 kb on + strand at 547.240 kb on + strand at 547.261 kb on - strand at 547.261 kb on - strand at 547.263 kb on + strand at 547.263 kb on + strand at 547.263 kb on - strand at 547.268 kb on + strand at 547.268 kb on + strand at 547.276 kb on - strand at 547.283 kb on - strand at 547.289 kb on + strand at 547.302 kb on - strand at 547.322 kb on + strand at 547.330 kb on - strand at 547.330 kb on - strand at 547.332 kb on + strand at 547.339 kb on - strand at 547.340 kb on - strand at 547.344 kb on + strand at 547.387 kb on + strand at 547.388 kb on - strand at 547.419 kb on - strand, within CCNA_00535 at 547.500 kb on + strand, within CCNA_00535 at 547.503 kb on + strand, within CCNA_00535 at 547.615 kb on + strand at 551.900 kb on + strand at 551.900 kb on + strand at 551.937 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml remove 546,904 + CCNA_00534 0.58 +0.6 546,928 - CCNA_00534 0.60 +3.4 546,931 + CCNA_00534 0.60 -0.5 546,931 + CCNA_00534 0.60 -0.8 546,935 + CCNA_00534 0.61 +0.0 546,939 - CCNA_00534 0.61 -0.5 546,939 - CCNA_00534 0.61 -0.4 546,939 - CCNA_00534 0.61 +0.5 546,950 + CCNA_00534 0.62 -0.0 547,055 + CCNA_00534 0.72 -1.0 547,066 - CCNA_00534 0.74 -1.8 547,082 + CCNA_00534 0.75 +0.3 547,082 + CCNA_00534 0.75 -3.1 547,084 + CCNA_00534 0.75 +1.6 547,090 - CCNA_00534 0.76 +0.4 547,096 - CCNA_00534 0.76 +1.6 547,151 - CCNA_00534 0.82 +1.2 547,181 + CCNA_00534 0.85 -0.3 547,181 + CCNA_00534 0.85 +0.8 547,216 - CCNA_00534 0.88 +1.2 547,240 + -0.7 547,240 + -0.1 547,240 + +1.6 547,261 - -0.0 547,261 - +1.4 547,263 + +0.3 547,263 + +1.6 547,263 - +1.6 547,268 + -0.1 547,268 + -0.8 547,276 - -1.3 547,283 - -0.2 547,289 + -0.3 547,302 - +0.4 547,322 + -1.6 547,330 - +0.7 547,330 - +0.5 547,332 + -1.5 547,339 - -0.3 547,340 - -1.0 547,344 + +1.2 547,387 + -0.4 547,388 - +0.5 547,419 - CCNA_00535 0.25 -0.7 547,500 + CCNA_00535 0.76 -2.2 547,503 + CCNA_00535 0.78 -0.8 547,615 + -0.4 551,900 + +0.2 551,900 + +0.5 551,937 + -0.5
Or see this region's nucleotide sequence