Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00517

Experiment: PYE with Spectinomycin 0.08 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00515 and CCNA_00516 are separated by 25 nucleotidesCCNA_00516 and CCNA_00517 are separated by 2 nucleotidesCCNA_00517 and CCNA_00518 are separated by 87 nucleotidesCCNA_00518 and CCNA_00519 are separated by 157 nucleotides CCNA_00515: CCNA_00515 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase, at 529,324 to 531,657 _00515 CCNA_00516: CCNA_00516 - integral membrane protein, at 531,683 to 532,432 _00516 CCNA_00517: CCNA_00517 - peptidyl-tRNA hydrolase, at 532,435 to 533,052 _00517 CCNA_00518: CCNA_00518 - LSU ribosomal protein L25P, at 533,140 to 533,733 _00518 CCNA_00519: CCNA_00519 - hypothetical protein, at 533,891 to 534,967 _00519 Position (kb) 532 533 534Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 531.442 kb on - strandat 531.445 kb on + strandat 531.445 kb on + strandat 531.453 kb on - strandat 531.462 kb on - strandat 531.463 kb on - strandat 531.471 kb on + strandat 531.512 kb on - strandat 531.514 kb on - strandat 531.552 kb on + strandat 531.552 kb on + strandat 531.552 kb on + strandat 531.552 kb on + strandat 531.560 kb on - strandat 531.563 kb on + strandat 531.582 kb on - strandat 531.584 kb on + strandat 531.660 kb on - strandat 531.682 kb on + strandat 531.717 kb on + strandat 531.725 kb on - strandat 531.746 kb on + strandat 531.746 kb on + strandat 531.747 kb on + strandat 531.755 kb on - strandat 531.755 kb on - strandat 531.755 kb on - strandat 531.825 kb on + strand, within CCNA_00516at 531.825 kb on + strand, within CCNA_00516at 531.833 kb on + strand, within CCNA_00516at 531.850 kb on - strand, within CCNA_00516at 531.870 kb on - strand, within CCNA_00516at 531.872 kb on + strand, within CCNA_00516at 531.872 kb on + strand, within CCNA_00516at 531.872 kb on + strand, within CCNA_00516at 531.880 kb on - strand, within CCNA_00516at 531.880 kb on - strand, within CCNA_00516at 531.880 kb on - strand, within CCNA_00516at 531.880 kb on - strand, within CCNA_00516at 531.882 kb on + strand, within CCNA_00516at 531.887 kb on + strand, within CCNA_00516at 531.890 kb on - strand, within CCNA_00516at 531.890 kb on - strand, within CCNA_00516at 531.895 kb on - strand, within CCNA_00516at 531.907 kb on + strand, within CCNA_00516at 531.915 kb on - strand, within CCNA_00516at 531.915 kb on - strand, within CCNA_00516at 531.915 kb on - strand, within CCNA_00516at 531.939 kb on + strand, within CCNA_00516at 531.939 kb on + strand, within CCNA_00516at 531.947 kb on - strand, within CCNA_00516at 531.947 kb on - strand, within CCNA_00516at 531.960 kb on + strand, within CCNA_00516at 531.967 kb on - strand, within CCNA_00516at 532.014 kb on + strand, within CCNA_00516at 532.019 kb on + strand, within CCNA_00516at 532.024 kb on + strand, within CCNA_00516at 532.037 kb on + strand, within CCNA_00516at 532.070 kb on - strand, within CCNA_00516at 532.070 kb on - strand, within CCNA_00516at 532.087 kb on - strand, within CCNA_00516at 532.109 kb on - strand, within CCNA_00516at 532.113 kb on - strand, within CCNA_00516at 532.177 kb on - strand, within CCNA_00516at 532.249 kb on - strand, within CCNA_00516at 532.266 kb on + strand, within CCNA_00516at 532.272 kb on + strand, within CCNA_00516at 532.275 kb on - strand, within CCNA_00516at 532.284 kb on - strand, within CCNA_00516at 532.298 kb on - strand, within CCNA_00516at 532.334 kb on + strand, within CCNA_00516at 532.337 kb on - strand, within CCNA_00516at 532.355 kb on + strand, within CCNA_00516at 532.355 kb on + strand, within CCNA_00516at 532.355 kb on + strand, within CCNA_00516at 532.363 kb on - strandat 532.383 kb on + strandat 532.403 kb on + strandat 532.461 kb on + strandat 532.461 kb on + strandat 532.461 kb on + strandat 532.474 kb on - strandat 532.501 kb on - strand, within CCNA_00517at 532.501 kb on - strand, within CCNA_00517at 532.501 kb on - strand, within CCNA_00517at 533.059 kb on + strandat 533.059 kb on + strandat 533.067 kb on - strandat 533.067 kb on - strandat 533.067 kb on - strandat 533.067 kb on - strandat 533.067 kb on - strandat 533.069 kb on - strandat 533.137 kb on + strandat 533.183 kb on + strandat 533.183 kb on + strandat 533.191 kb on - strandat 533.191 kb on - strandat 533.191 kb on - strandat 533.191 kb on - strandat 533.342 kb on - strand, within CCNA_00518at 533.342 kb on - strand, within CCNA_00518at 533.342 kb on - strand, within CCNA_00518at 533.363 kb on - strand, within CCNA_00518at 533.363 kb on - strand, within CCNA_00518at 533.377 kb on - strand, within CCNA_00518at 533.921 kb on + strandat 533.921 kb on + strandat 533.921 kb on + strandat 533.921 kb on + strandat 533.921 kb on + strandat 533.922 kb on + strandat 533.929 kb on - strandat 533.929 kb on - strandat 533.929 kb on - strandat 533.929 kb on - strandat 533.977 kb on + strandat 534.015 kb on - strand, within CCNA_00519at 534.015 kb on - strand, within CCNA_00519at 534.050 kb on - strand, within CCNA_00519

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml
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531,442 - +0.1
531,445 + +0.1
531,445 + +1.9
531,453 - -0.6
531,462 - +0.4
531,463 - -0.3
531,471 + -0.6
531,512 - +0.9
531,514 - -0.7
531,552 + +0.1
531,552 + +2.0
531,552 + +1.0
531,552 + -0.4
531,560 - -0.6
531,563 + -0.2
531,582 - -2.6
531,584 + +0.1
531,660 - +2.3
531,682 + -0.3
531,717 + +1.3
531,725 - +0.9
531,746 + -1.2
531,746 + -0.4
531,747 + +0.5
531,755 - -0.4
531,755 - -1.1
531,755 - +0.1
531,825 + CCNA_00516 0.19 +0.4
531,825 + CCNA_00516 0.19 -0.5
531,833 + CCNA_00516 0.20 -0.8
531,850 - CCNA_00516 0.22 -1.4
531,870 - CCNA_00516 0.25 -1.6
531,872 + CCNA_00516 0.25 -1.2
531,872 + CCNA_00516 0.25 -1.3
531,872 + CCNA_00516 0.25 -0.1
531,880 - CCNA_00516 0.26 -0.5
531,880 - CCNA_00516 0.26 -0.1
531,880 - CCNA_00516 0.26 -0.6
531,880 - CCNA_00516 0.26 +0.0
531,882 + CCNA_00516 0.27 +2.3
531,887 + CCNA_00516 0.27 +2.5
531,890 - CCNA_00516 0.28 -0.6
531,890 - CCNA_00516 0.28 -0.0
531,895 - CCNA_00516 0.28 -0.4
531,907 + CCNA_00516 0.30 -1.1
531,915 - CCNA_00516 0.31 -3.1
531,915 - CCNA_00516 0.31 -0.2
531,915 - CCNA_00516 0.31 +0.4
531,939 + CCNA_00516 0.34 +0.1
531,939 + CCNA_00516 0.34 -1.4
531,947 - CCNA_00516 0.35 -2.3
531,947 - CCNA_00516 0.35 +0.3
531,960 + CCNA_00516 0.37 +1.5
531,967 - CCNA_00516 0.38 -1.3
532,014 + CCNA_00516 0.44 -1.4
532,019 + CCNA_00516 0.45 -1.1
532,024 + CCNA_00516 0.45 -1.7
532,037 + CCNA_00516 0.47 +0.0
532,070 - CCNA_00516 0.52 -2.0
532,070 - CCNA_00516 0.52 -1.6
532,087 - CCNA_00516 0.54 -0.9
532,109 - CCNA_00516 0.57 -0.6
532,113 - CCNA_00516 0.57 +1.7
532,177 - CCNA_00516 0.66 +0.9
532,249 - CCNA_00516 0.75 +1.3
532,266 + CCNA_00516 0.78 +0.1
532,272 + CCNA_00516 0.79 +0.6
532,275 - CCNA_00516 0.79 +0.5
532,284 - CCNA_00516 0.80 -0.4
532,298 - CCNA_00516 0.82 -0.4
532,334 + CCNA_00516 0.87 +2.7
532,337 - CCNA_00516 0.87 -1.0
532,355 + CCNA_00516 0.90 -1.9
532,355 + CCNA_00516 0.90 +3.9
532,355 + CCNA_00516 0.90 +1.3
532,363 - -2.3
532,383 + +0.1
532,403 + +1.8
532,461 + +0.1
532,461 + +1.0
532,461 + +0.8
532,474 - +0.6
532,501 - CCNA_00517 0.11 -0.5
532,501 - CCNA_00517 0.11 -1.4
532,501 - CCNA_00517 0.11 +1.5
533,059 + +0.2
533,059 + -1.3
533,067 - -0.4
533,067 - +0.6
533,067 - +0.7
533,067 - +0.3
533,067 - +0.5
533,069 - -0.7
533,137 + -0.5
533,183 + -0.5
533,183 + -0.1
533,191 - +0.9
533,191 - +0.9
533,191 - +1.3
533,191 - +1.0
533,342 - CCNA_00518 0.34 +1.2
533,342 - CCNA_00518 0.34 +0.4
533,342 - CCNA_00518 0.34 +1.0
533,363 - CCNA_00518 0.38 +0.8
533,363 - CCNA_00518 0.38 +0.7
533,377 - CCNA_00518 0.40 +1.1
533,921 + -0.7
533,921 + -0.7
533,921 + -0.6
533,921 + +1.2
533,921 + -1.4
533,922 + -0.6
533,929 - +0.9
533,929 - -0.2
533,929 - -0.0
533,929 - +0.8
533,977 + +0.6
534,015 - CCNA_00519 0.12 +1.2
534,015 - CCNA_00519 0.12 +0.5
534,050 - CCNA_00519 0.15 -1.2

Or see this region's nucleotide sequence