Experiment: PYE with Spectinomycin 0.08 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00408 and CCNA_00409 are separated by 97 nucleotides CCNA_00409 and CCNA_00410 are separated by 62 nucleotides CCNA_00410 and CCNA_00411 are separated by 32 nucleotides CCNA_00411 and CCNA_00412 overlap by 4 nucleotides CCNA_00412 and CCNA_00413 are separated by 92 nucleotides
CCNA_00408: CCNA_00408 - antiporter protein, at 423,040 to 424,308
_00408
CCNA_00409: CCNA_00409 - hypothetical protein, at 424,406 to 424,450
_00409
CCNA_00410: CCNA_00410 - acetyltransferase, at 424,513 to 425,043
_00410
CCNA_00411: CCNA_00411 - hypothetical protein, at 425,076 to 425,639
_00411
CCNA_00412: CCNA_00412 - CoA pyrophosphatase, at 425,636 to 426,286
_00412
CCNA_00413: CCNA_00413 - poly(A) polymerase/tRNA-nucleotidyltransferase, at 426,379 to 427,632
_00413
Position (kb) 425
426 Strain fitness (log2 ratio) -2
-1
0
1
2 at 424.076 kb on + strand, within CCNA_00408 at 424.092 kb on + strand, within CCNA_00408 at 424.159 kb on - strand, within CCNA_00408 at 424.159 kb on - strand, within CCNA_00408 at 424.159 kb on - strand, within CCNA_00408 at 424.168 kb on - strand, within CCNA_00408 at 424.199 kb on - strand at 424.205 kb on - strand at 424.205 kb on - strand at 424.205 kb on - strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.222 kb on + strand at 424.225 kb on - strand at 424.225 kb on - strand at 424.225 kb on - strand at 424.225 kb on - strand at 424.230 kb on - strand at 424.230 kb on - strand at 424.264 kb on + strand at 424.342 kb on - strand at 424.346 kb on + strand at 424.363 kb on + strand at 424.365 kb on - strand at 424.368 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.371 kb on - strand at 424.377 kb on - strand at 424.377 kb on - strand at 424.377 kb on - strand at 424.404 kb on - strand at 424.445 kb on + strand, within CCNA_00409 at 424.452 kb on + strand at 424.465 kb on + strand at 424.473 kb on - strand at 424.473 kb on - strand at 424.481 kb on + strand at 424.489 kb on + strand at 424.530 kb on - strand at 424.728 kb on + strand, within CCNA_00410 at 424.795 kb on - strand, within CCNA_00410 at 424.999 kb on + strand at 425.010 kb on + strand at 425.024 kb on + strand at 425.032 kb on - strand at 425.066 kb on + strand at 425.073 kb on + strand at 425.081 kb on - strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.090 kb on + strand at 425.090 kb on + strand at 425.090 kb on + strand at 425.090 kb on + strand at 425.096 kb on - strand at 425.096 kb on - strand at 425.100 kb on + strand at 425.100 kb on + strand at 425.100 kb on + strand at 425.103 kb on - strand at 425.110 kb on + strand at 425.120 kb on + strand at 425.140 kb on + strand, within CCNA_00411 at 425.165 kb on - strand, within CCNA_00411 at 425.221 kb on + strand, within CCNA_00411 at 425.248 kb on - strand, within CCNA_00411 at 425.299 kb on + strand, within CCNA_00411 at 425.299 kb on + strand, within CCNA_00411 at 425.307 kb on - strand, within CCNA_00411 at 425.314 kb on - strand, within CCNA_00411 at 425.335 kb on - strand, within CCNA_00411 at 425.354 kb on + strand, within CCNA_00411 at 425.362 kb on - strand, within CCNA_00411 at 425.362 kb on - strand, within CCNA_00411 at 425.362 kb on - strand, within CCNA_00411 at 425.367 kb on + strand, within CCNA_00411 at 425.377 kb on + strand, within CCNA_00411 at 425.465 kb on + strand, within CCNA_00411 at 425.505 kb on + strand, within CCNA_00411 at 425.536 kb on + strand, within CCNA_00411 at 425.536 kb on + strand, within CCNA_00411 at 425.559 kb on + strand, within CCNA_00411 at 425.628 kb on + strand at 425.628 kb on + strand at 425.628 kb on + strand at 425.629 kb on - strand at 425.633 kb on + strand at 425.636 kb on - strand at 425.719 kb on + strand, within CCNA_00412 at 425.800 kb on + strand, within CCNA_00412 at 425.800 kb on + strand, within CCNA_00412 at 425.811 kb on + strand, within CCNA_00412 at 425.836 kb on - strand, within CCNA_00412 at 425.849 kb on + strand, within CCNA_00412 at 425.865 kb on - strand, within CCNA_00412 at 425.900 kb on - strand, within CCNA_00412 at 425.920 kb on - strand, within CCNA_00412 at 425.920 kb on - strand, within CCNA_00412 at 425.924 kb on + strand, within CCNA_00412 at 425.924 kb on + strand, within CCNA_00412 at 425.932 kb on - strand, within CCNA_00412 at 425.960 kb on - strand, within CCNA_00412 at 425.968 kb on + strand, within CCNA_00412 at 425.987 kb on + strand, within CCNA_00412 at 426.012 kb on + strand, within CCNA_00412 at 426.057 kb on + strand, within CCNA_00412 at 426.064 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.119 kb on + strand, within CCNA_00412 at 426.137 kb on + strand, within CCNA_00412 at 426.195 kb on + strand, within CCNA_00412 at 426.213 kb on - strand, within CCNA_00412 at 426.241 kb on + strand at 426.241 kb on + strand at 426.271 kb on + strand at 426.276 kb on + strand at 426.276 kb on + strand at 426.276 kb on + strand at 426.276 kb on + strand at 426.284 kb on - strand at 426.284 kb on - strand at 426.318 kb on - strand at 426.321 kb on - strand at 426.329 kb on + strand at 426.344 kb on - strand at 426.345 kb on - strand at 426.376 kb on + strand at 426.384 kb on - strand at 426.391 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml remove 424,076 + CCNA_00408 0.82 -0.2 424,092 + CCNA_00408 0.83 +0.2 424,159 - CCNA_00408 0.88 -1.1 424,159 - CCNA_00408 0.88 -0.5 424,159 - CCNA_00408 0.88 +2.2 424,168 - CCNA_00408 0.89 +0.7 424,199 - +0.6 424,205 - -0.7 424,205 - +1.7 424,205 - +0.1 424,217 + -0.9 424,217 + -1.3 424,217 + -0.4 424,217 + +2.1 424,217 + +0.5 424,217 + -0.1 424,217 + +1.4 424,222 + -0.9 424,225 - -0.2 424,225 - +1.1 424,225 - -1.7 424,225 - +0.2 424,230 - +0.5 424,230 - -0.3 424,264 + -0.3 424,342 - +1.0 424,346 + -0.2 424,363 + -0.3 424,365 - -0.3 424,368 + +0.3 424,369 + -0.1 424,369 + +0.7 424,369 + -0.1 424,369 + -0.1 424,369 + +0.3 424,369 + +0.1 424,371 - -1.9 424,377 - -1.5 424,377 - -0.9 424,377 - +2.1 424,404 - +1.7 424,445 + CCNA_00409 0.87 -0.2 424,452 + +1.0 424,465 + +2.1 424,473 - -1.9 424,473 - -0.7 424,481 + +0.8 424,489 + -0.8 424,530 - +0.1 424,728 + CCNA_00410 0.40 +1.8 424,795 - CCNA_00410 0.53 +0.4 424,999 + +0.4 425,010 + -1.7 425,024 + -1.0 425,032 - +1.5 425,066 + -0.4 425,073 + -0.4 425,081 - -1.4 425,088 + -1.0 425,088 + -0.2 425,088 + +0.3 425,088 + +1.1 425,088 + -0.5 425,088 + -0.8 425,090 + +1.5 425,090 + -0.0 425,090 + -1.0 425,090 + -1.5 425,096 - +0.1 425,096 - +0.1 425,100 + +0.8 425,100 + -0.7 425,100 + -0.9 425,103 - -0.5 425,110 + +0.4 425,120 + +0.7 425,140 + CCNA_00411 0.11 -0.0 425,165 - CCNA_00411 0.16 -1.5 425,221 + CCNA_00411 0.26 -0.4 425,248 - CCNA_00411 0.30 -1.9 425,299 + CCNA_00411 0.40 +2.2 425,299 + CCNA_00411 0.40 +0.5 425,307 - CCNA_00411 0.41 +0.6 425,314 - CCNA_00411 0.42 -1.1 425,335 - CCNA_00411 0.46 +1.5 425,354 + CCNA_00411 0.49 +0.8 425,362 - CCNA_00411 0.51 -1.5 425,362 - CCNA_00411 0.51 +0.9 425,362 - CCNA_00411 0.51 +1.1 425,367 + CCNA_00411 0.52 +0.1 425,377 + CCNA_00411 0.53 +0.4 425,465 + CCNA_00411 0.69 +0.0 425,505 + CCNA_00411 0.76 +0.0 425,536 + CCNA_00411 0.82 -1.2 425,536 + CCNA_00411 0.82 +0.6 425,559 + CCNA_00411 0.86 -0.1 425,628 + -0.4 425,628 + -1.1 425,628 + -0.1 425,629 - +0.4 425,633 + -0.3 425,636 - +1.7 425,719 + CCNA_00412 0.13 +1.3 425,800 + CCNA_00412 0.25 -0.8 425,800 + CCNA_00412 0.25 -0.2 425,811 + CCNA_00412 0.27 +0.0 425,836 - CCNA_00412 0.31 +0.7 425,849 + CCNA_00412 0.33 +0.2 425,865 - CCNA_00412 0.35 +1.7 425,900 - CCNA_00412 0.41 -1.4 425,920 - CCNA_00412 0.44 +0.1 425,920 - CCNA_00412 0.44 -0.1 425,924 + CCNA_00412 0.44 -0.4 425,924 + CCNA_00412 0.44 +0.2 425,932 - CCNA_00412 0.45 -0.0 425,960 - CCNA_00412 0.50 -0.5 425,968 + CCNA_00412 0.51 +0.6 425,987 + CCNA_00412 0.54 +0.5 426,012 + CCNA_00412 0.58 -0.0 426,057 + CCNA_00412 0.65 +2.1 426,064 + CCNA_00412 0.66 -0.2 426,069 + CCNA_00412 0.67 +0.0 426,069 + CCNA_00412 0.67 +0.4 426,069 + CCNA_00412 0.67 -0.4 426,069 + CCNA_00412 0.67 +0.3 426,069 + CCNA_00412 0.67 -0.3 426,069 + CCNA_00412 0.67 +0.0 426,077 - CCNA_00412 0.68 -0.2 426,077 - CCNA_00412 0.68 -1.3 426,077 - CCNA_00412 0.68 +0.4 426,077 - CCNA_00412 0.68 -0.7 426,077 - CCNA_00412 0.68 +0.5 426,077 - CCNA_00412 0.68 -0.2 426,077 - CCNA_00412 0.68 +2.0 426,119 + CCNA_00412 0.74 +0.1 426,137 + CCNA_00412 0.77 +1.4 426,195 + CCNA_00412 0.86 +0.2 426,213 - CCNA_00412 0.89 -0.5 426,241 + +1.0 426,241 + -1.5 426,271 + +0.4 426,276 + +1.1 426,276 + -0.1 426,276 + +0.0 426,276 + -0.7 426,284 - -0.1 426,284 - +0.5 426,318 - -1.4 426,321 - -0.3 426,329 + -1.1 426,344 - +0.0 426,345 - -1.6 426,376 + +2.5 426,384 - -1.1 426,391 - +1.2
Or see this region's nucleotide sequence