Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00179

Experiment: PYE with Spectinomycin 0.08 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00176 and CCNA_00177 overlap by 32 nucleotidesCCNA_00177 and CCNA_00178 overlap by 4 nucleotidesCCNA_00178 and CCNA_00179 overlap by 4 nucleotidesCCNA_00179 and CCNA_00180 overlap by 19 nucleotides CCNA_00176: CCNA_00176 - type II secretion pathway protein H, at 191,399 to 191,956 _00176 CCNA_00177: CCNA_00177 - type II secretion pathway protein I, at 191,925 to 192,308 _00177 CCNA_00178: CCNA_00178 - type II secretion pathway protein J, at 192,305 to 192,895 _00178 CCNA_00179: CCNA_00179 - type II secretion pathway protein K, at 192,892 to 193,866 _00179 CCNA_00180: CCNA_00180 - type II secretion pathway protein L, at 193,848 to 194,954 _00180 Position (kb) 192 193 194Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 191.919 kb on + strandat 191.962 kb on + strandat 192.018 kb on + strand, within CCNA_00177at 192.080 kb on + strand, within CCNA_00177at 192.138 kb on + strand, within CCNA_00177at 192.138 kb on + strand, within CCNA_00177at 192.146 kb on - strand, within CCNA_00177at 192.146 kb on - strand, within CCNA_00177at 192.146 kb on - strand, within CCNA_00177at 192.146 kb on - strand, within CCNA_00177at 192.184 kb on + strand, within CCNA_00177at 192.198 kb on + strand, within CCNA_00177at 192.251 kb on - strand, within CCNA_00177at 192.251 kb on - strand, within CCNA_00177at 192.290 kb on + strandat 192.290 kb on + strandat 192.290 kb on + strandat 192.290 kb on + strandat 192.290 kb on + strandat 192.290 kb on + strandat 192.298 kb on - strandat 192.298 kb on - strandat 192.298 kb on - strandat 192.310 kb on + strandat 192.312 kb on + strandat 192.320 kb on - strandat 192.341 kb on + strandat 192.349 kb on - strandat 192.366 kb on - strand, within CCNA_00178at 192.385 kb on - strand, within CCNA_00178at 192.395 kb on - strand, within CCNA_00178at 192.435 kb on + strand, within CCNA_00178at 192.486 kb on - strand, within CCNA_00178at 192.553 kb on - strand, within CCNA_00178at 192.579 kb on + strand, within CCNA_00178at 192.592 kb on - strand, within CCNA_00178at 192.622 kb on - strand, within CCNA_00178at 192.631 kb on + strandat 192.650 kb on - strand, within CCNA_00178at 192.652 kb on + strand, within CCNA_00178at 192.653 kb on + strand, within CCNA_00178at 192.653 kb on + strand, within CCNA_00178at 192.660 kb on - strand, within CCNA_00178at 192.661 kb on - strand, within CCNA_00178at 192.694 kb on - strand, within CCNA_00178at 192.696 kb on - strand, within CCNA_00178at 192.699 kb on - strand, within CCNA_00178at 192.699 kb on - strand, within CCNA_00178at 192.702 kb on + strand, within CCNA_00178at 192.729 kb on - strand, within CCNA_00178at 192.758 kb on - strand, within CCNA_00178at 192.769 kb on + strand, within CCNA_00178at 192.771 kb on - strand, within CCNA_00178at 192.776 kb on - strand, within CCNA_00178at 192.784 kb on - strand, within CCNA_00178at 192.818 kb on + strand, within CCNA_00178at 192.824 kb on + strand, within CCNA_00178at 192.832 kb on - strand, within CCNA_00178at 192.876 kb on + strandat 192.876 kb on + strandat 192.876 kb on - strandat 192.878 kb on - strandat 192.884 kb on - strandat 192.884 kb on - strandat 192.884 kb on - strandat 192.886 kb on + strandat 192.912 kb on - strandat 192.919 kb on + strandat 192.942 kb on - strandat 193.022 kb on + strand, within CCNA_00179at 193.041 kb on - strand, within CCNA_00179at 193.046 kb on - strand, within CCNA_00179at 193.052 kb on + strand, within CCNA_00179at 193.055 kb on - strand, within CCNA_00179at 193.112 kb on - strand, within CCNA_00179at 193.112 kb on - strand, within CCNA_00179at 193.113 kb on - strand, within CCNA_00179at 193.117 kb on - strand, within CCNA_00179at 193.123 kb on - strand, within CCNA_00179at 193.123 kb on - strand, within CCNA_00179at 193.164 kb on + strand, within CCNA_00179at 193.177 kb on - strand, within CCNA_00179at 193.183 kb on - strand, within CCNA_00179at 193.184 kb on - strand, within CCNA_00179at 193.191 kb on - strand, within CCNA_00179at 193.202 kb on + strand, within CCNA_00179at 193.215 kb on - strand, within CCNA_00179at 193.265 kb on + strand, within CCNA_00179at 193.275 kb on + strand, within CCNA_00179at 193.283 kb on - strand, within CCNA_00179at 193.290 kb on - strand, within CCNA_00179at 193.313 kb on + strand, within CCNA_00179at 193.321 kb on - strand, within CCNA_00179at 193.350 kb on + strand, within CCNA_00179at 193.352 kb on + strand, within CCNA_00179at 193.402 kb on + strand, within CCNA_00179at 193.404 kb on - strand, within CCNA_00179at 193.410 kb on - strand, within CCNA_00179at 193.448 kb on - strand, within CCNA_00179at 193.466 kb on + strand, within CCNA_00179at 193.477 kb on + strand, within CCNA_00179at 193.479 kb on + strand, within CCNA_00179at 193.487 kb on - strand, within CCNA_00179at 193.487 kb on - strand, within CCNA_00179at 193.487 kb on - strand, within CCNA_00179at 193.530 kb on - strand, within CCNA_00179at 193.538 kb on - strand, within CCNA_00179at 193.542 kb on + strand, within CCNA_00179at 193.578 kb on - strand, within CCNA_00179at 193.590 kb on - strand, within CCNA_00179at 193.604 kb on + strand, within CCNA_00179at 193.650 kb on - strand, within CCNA_00179at 193.660 kb on + strand, within CCNA_00179at 193.676 kb on - strand, within CCNA_00179at 193.695 kb on - strand, within CCNA_00179at 193.772 kb on - strandat 193.788 kb on + strandat 193.788 kb on + strandat 193.796 kb on - strandat 193.845 kb on + strandat 193.853 kb on - strandat 193.866 kb on + strandat 193.867 kb on - strandat 193.878 kb on + strandat 193.878 kb on + strandat 193.920 kb on + strandat 193.925 kb on + strandat 193.927 kb on + strandat 193.927 kb on + strandat 193.927 kb on + strandat 193.927 kb on - strandat 193.933 kb on - strandat 193.933 kb on - strandat 193.933 kb on - strandat 193.935 kb on - strandat 193.947 kb on - strandat 193.955 kb on - strandat 193.982 kb on + strand, within CCNA_00180at 193.990 kb on + strand, within CCNA_00180at 193.994 kb on + strand, within CCNA_00180at 194.032 kb on + strand, within CCNA_00180at 194.040 kb on - strand, within CCNA_00180at 194.053 kb on + strand, within CCNA_00180at 194.058 kb on + strand, within CCNA_00180at 194.066 kb on - strand, within CCNA_00180at 194.066 kb on - strand, within CCNA_00180at 194.098 kb on + strand, within CCNA_00180at 194.118 kb on + strand, within CCNA_00180at 194.161 kb on + strand, within CCNA_00180at 194.163 kb on - strand, within CCNA_00180at 194.168 kb on + strand, within CCNA_00180at 194.168 kb on + strand, within CCNA_00180at 194.168 kb on + strand, within CCNA_00180at 194.176 kb on - strand, within CCNA_00180at 194.178 kb on + strand, within CCNA_00180at 194.186 kb on - strand, within CCNA_00180at 194.190 kb on + strand, within CCNA_00180at 194.198 kb on - strand, within CCNA_00180at 194.198 kb on - strand, within CCNA_00180at 194.198 kb on - strand, within CCNA_00180at 194.211 kb on + strand, within CCNA_00180at 194.211 kb on + strand, within CCNA_00180at 194.211 kb on + strand, within CCNA_00180at 194.211 kb on + strand, within CCNA_00180at 194.219 kb on - strand, within CCNA_00180at 194.219 kb on - strand, within CCNA_00180at 194.219 kb on - strand, within CCNA_00180at 194.219 kb on - strand, within CCNA_00180at 194.219 kb on - strand, within CCNA_00180at 194.269 kb on + strand, within CCNA_00180at 194.277 kb on - strand, within CCNA_00180at 194.283 kb on + strand, within CCNA_00180at 194.335 kb on - strand, within CCNA_00180at 194.335 kb on - strand, within CCNA_00180at 194.390 kb on + strand, within CCNA_00180at 194.399 kb on + strand, within CCNA_00180at 194.403 kb on + strand, within CCNA_00180at 194.456 kb on - strand, within CCNA_00180at 194.510 kb on - strand, within CCNA_00180at 194.540 kb on + strand, within CCNA_00180at 194.546 kb on - strand, within CCNA_00180at 194.548 kb on - strand, within CCNA_00180at 194.638 kb on - strand, within CCNA_00180at 194.640 kb on - strand, within CCNA_00180at 194.671 kb on + strand, within CCNA_00180at 194.671 kb on + strand, within CCNA_00180at 194.677 kb on - strand, within CCNA_00180at 194.679 kb on - strand, within CCNA_00180at 194.719 kb on - strand, within CCNA_00180at 194.752 kb on + strand, within CCNA_00180at 194.758 kb on + strand, within CCNA_00180at 194.777 kb on - strand, within CCNA_00180at 194.779 kb on + strand, within CCNA_00180at 194.779 kb on + strand, within CCNA_00180at 194.779 kb on + strand, within CCNA_00180at 194.787 kb on - strand, within CCNA_00180at 194.787 kb on - strand, within CCNA_00180at 194.787 kb on - strand, within CCNA_00180at 194.847 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.08 mg/ml
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191,919 + -0.5
191,962 + -1.4
192,018 + CCNA_00177 0.24 +1.5
192,080 + CCNA_00177 0.40 -1.2
192,138 + CCNA_00177 0.55 +1.0
192,138 + CCNA_00177 0.55 -0.2
192,146 - CCNA_00177 0.58 -0.7
192,146 - CCNA_00177 0.58 -1.4
192,146 - CCNA_00177 0.58 +0.6
192,146 - CCNA_00177 0.58 -0.6
192,184 + CCNA_00177 0.67 -0.9
192,198 + CCNA_00177 0.71 +0.6
192,251 - CCNA_00177 0.85 +0.3
192,251 - CCNA_00177 0.85 -0.8
192,290 + -1.4
192,290 + +0.2
192,290 + -1.1
192,290 + -0.7
192,290 + +1.5
192,290 + +0.5
192,298 - +0.7
192,298 - +0.5
192,298 - -0.2
192,310 + -2.2
192,312 + -0.2
192,320 - +1.3
192,341 + -0.3
192,349 - +0.5
192,366 - CCNA_00178 0.10 -1.2
192,385 - CCNA_00178 0.14 -1.5
192,395 - CCNA_00178 0.15 -0.3
192,435 + CCNA_00178 0.22 +0.6
192,486 - CCNA_00178 0.31 +0.5
192,553 - CCNA_00178 0.42 +1.6
192,579 + CCNA_00178 0.46 -0.3
192,592 - CCNA_00178 0.49 -0.2
192,622 - CCNA_00178 0.54 +0.2
192,631 + +1.1
192,650 - CCNA_00178 0.58 -0.9
192,652 + CCNA_00178 0.59 -0.9
192,653 + CCNA_00178 0.59 -0.6
192,653 + CCNA_00178 0.59 -1.5
192,660 - CCNA_00178 0.60 +0.4
192,661 - CCNA_00178 0.60 -1.4
192,694 - CCNA_00178 0.66 -1.5
192,696 - CCNA_00178 0.66 -0.7
192,699 - CCNA_00178 0.67 -0.2
192,699 - CCNA_00178 0.67 -0.6
192,702 + CCNA_00178 0.67 -0.8
192,729 - CCNA_00178 0.72 +0.5
192,758 - CCNA_00178 0.77 +1.5
192,769 + CCNA_00178 0.79 -0.1
192,771 - CCNA_00178 0.79 +1.8
192,776 - CCNA_00178 0.80 -0.3
192,784 - CCNA_00178 0.81 +0.3
192,818 + CCNA_00178 0.87 -0.4
192,824 + CCNA_00178 0.88 +0.1
192,832 - CCNA_00178 0.89 +0.4
192,876 + -1.4
192,876 + -2.5
192,876 - -0.9
192,878 - -0.5
192,884 - -0.5
192,884 - -1.0
192,884 - +0.6
192,886 + +0.5
192,912 - -1.4
192,919 + -0.2
192,942 - -0.1
193,022 + CCNA_00179 0.13 -0.0
193,041 - CCNA_00179 0.15 +0.3
193,046 - CCNA_00179 0.16 -0.6
193,052 + CCNA_00179 0.16 -0.0
193,055 - CCNA_00179 0.17 -0.7
193,112 - CCNA_00179 0.23 +0.1
193,112 - CCNA_00179 0.23 -1.1
193,113 - CCNA_00179 0.23 +0.4
193,117 - CCNA_00179 0.23 -1.6
193,123 - CCNA_00179 0.24 -0.1
193,123 - CCNA_00179 0.24 -1.6
193,164 + CCNA_00179 0.28 +1.3
193,177 - CCNA_00179 0.29 -0.2
193,183 - CCNA_00179 0.30 -0.2
193,184 - CCNA_00179 0.30 -0.0
193,191 - CCNA_00179 0.31 +0.5
193,202 + CCNA_00179 0.32 -2.5
193,215 - CCNA_00179 0.33 -0.7
193,265 + CCNA_00179 0.38 -0.5
193,275 + CCNA_00179 0.39 -0.5
193,283 - CCNA_00179 0.40 -0.7
193,290 - CCNA_00179 0.41 +2.8
193,313 + CCNA_00179 0.43 -0.6
193,321 - CCNA_00179 0.44 +1.0
193,350 + CCNA_00179 0.47 +1.0
193,352 + CCNA_00179 0.47 +0.3
193,402 + CCNA_00179 0.52 -0.7
193,404 - CCNA_00179 0.53 -1.6
193,410 - CCNA_00179 0.53 +0.3
193,448 - CCNA_00179 0.57 +0.2
193,466 + CCNA_00179 0.59 -0.0
193,477 + CCNA_00179 0.60 +0.7
193,479 + CCNA_00179 0.60 -0.8
193,487 - CCNA_00179 0.61 -0.1
193,487 - CCNA_00179 0.61 -0.7
193,487 - CCNA_00179 0.61 +0.4
193,530 - CCNA_00179 0.65 -0.3
193,538 - CCNA_00179 0.66 +0.3
193,542 + CCNA_00179 0.67 +1.1
193,578 - CCNA_00179 0.70 -0.9
193,590 - CCNA_00179 0.72 +4.1
193,604 + CCNA_00179 0.73 -1.1
193,650 - CCNA_00179 0.78 +0.2
193,660 + CCNA_00179 0.79 -0.9
193,676 - CCNA_00179 0.80 -1.2
193,695 - CCNA_00179 0.82 -1.0
193,772 - +0.2
193,788 + -0.0
193,788 + -2.2
193,796 - -0.3
193,845 + +0.5
193,853 - -0.7
193,866 + +0.7
193,867 - +0.3
193,878 + -0.6
193,878 + -0.4
193,920 + +1.0
193,925 + +0.7
193,927 + -0.8
193,927 + -0.7
193,927 + -0.8
193,927 - -0.5
193,933 - +1.0
193,933 - -0.2
193,933 - -0.6
193,935 - -0.7
193,947 - +0.4
193,955 - +1.5
193,982 + CCNA_00180 0.12 -0.6
193,990 + CCNA_00180 0.13 -0.9
193,994 + CCNA_00180 0.13 -0.8
194,032 + CCNA_00180 0.17 +0.7
194,040 - CCNA_00180 0.17 -0.0
194,053 + CCNA_00180 0.19 +1.8
194,058 + CCNA_00180 0.19 -1.2
194,066 - CCNA_00180 0.20 -0.1
194,066 - CCNA_00180 0.20 -1.0
194,098 + CCNA_00180 0.23 +0.0
194,118 + CCNA_00180 0.24 -0.7
194,161 + CCNA_00180 0.28 +1.8
194,163 - CCNA_00180 0.28 -1.8
194,168 + CCNA_00180 0.29 -0.7
194,168 + CCNA_00180 0.29 +0.7
194,168 + CCNA_00180 0.29 -0.6
194,176 - CCNA_00180 0.30 +3.5
194,178 + CCNA_00180 0.30 +2.0
194,186 - CCNA_00180 0.31 -0.2
194,190 + CCNA_00180 0.31 -0.7
194,198 - CCNA_00180 0.32 -0.3
194,198 - CCNA_00180 0.32 -0.2
194,198 - CCNA_00180 0.32 +0.9
194,211 + CCNA_00180 0.33 +0.5
194,211 + CCNA_00180 0.33 -1.3
194,211 + CCNA_00180 0.33 +2.1
194,211 + CCNA_00180 0.33 -0.3
194,219 - CCNA_00180 0.34 -1.3
194,219 - CCNA_00180 0.34 +0.8
194,219 - CCNA_00180 0.34 -0.9
194,219 - CCNA_00180 0.34 +0.2
194,219 - CCNA_00180 0.34 -0.4
194,269 + CCNA_00180 0.38 +0.4
194,277 - CCNA_00180 0.39 -2.1
194,283 + CCNA_00180 0.39 +0.7
194,335 - CCNA_00180 0.44 +0.7
194,335 - CCNA_00180 0.44 +0.2
194,390 + CCNA_00180 0.49 +0.4
194,399 + CCNA_00180 0.50 +0.2
194,403 + CCNA_00180 0.50 +1.8
194,456 - CCNA_00180 0.55 +0.3
194,510 - CCNA_00180 0.60 +0.3
194,540 + CCNA_00180 0.63 -0.4
194,546 - CCNA_00180 0.63 +0.8
194,548 - CCNA_00180 0.63 +0.2
194,638 - CCNA_00180 0.71 +0.5
194,640 - CCNA_00180 0.72 +0.3
194,671 + CCNA_00180 0.74 -0.6
194,671 + CCNA_00180 0.74 +1.1
194,677 - CCNA_00180 0.75 -0.6
194,679 - CCNA_00180 0.75 +0.3
194,719 - CCNA_00180 0.79 -1.2
194,752 + CCNA_00180 0.82 -0.5
194,758 + CCNA_00180 0.82 -0.4
194,777 - CCNA_00180 0.84 -1.2
194,779 + CCNA_00180 0.84 +2.3
194,779 + CCNA_00180 0.84 -1.4
194,779 + CCNA_00180 0.84 +0.8
194,787 - CCNA_00180 0.85 -0.8
194,787 - CCNA_00180 0.85 +0.7
194,787 - CCNA_00180 0.85 -0.1
194,847 + +1.8

Or see this region's nucleotide sequence