Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_23775 and AO353_23780 are separated by 553 nucleotides AO353_23780 and AO353_23785 are separated by 100 nucleotides AO353_23785 and AO353_23790 overlap by 1 nucleotides
AO353_23775: AO353_23775 - glycogen synthase, at 5,215,435 to 5,217,003
_23775
AO353_23780: AO353_23780 - hydroxyacid dehydrogenase, at 5,217,557 to 5,218,510
_23780
AO353_23785: AO353_23785 - hypothetical protein, at 5,218,611 to 5,219,045
_23785
AO353_23790: AO353_23790 - metal-binding protein, at 5,219,045 to 5,219,488
_23790
Position (kb) 5217
5218
5219 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5216.606 kb on + strand, within AO353_23775 at 5216.606 kb on + strand, within AO353_23775 at 5216.607 kb on - strand at 5216.630 kb on + strand, within AO353_23775 at 5216.630 kb on + strand, within AO353_23775 at 5216.630 kb on + strand, within AO353_23775 at 5216.630 kb on + strand, within AO353_23775 at 5216.630 kb on + strand, within AO353_23775 at 5216.630 kb on + strand, within AO353_23775 at 5216.630 kb on + strand, within AO353_23775 at 5216.630 kb on + strand, within AO353_23775 at 5216.630 kb on + strand, within AO353_23775 at 5216.631 kb on - strand, within AO353_23775 at 5216.631 kb on - strand, within AO353_23775 at 5216.631 kb on - strand at 5216.986 kb on + strand at 5216.986 kb on + strand at 5217.019 kb on + strand at 5217.019 kb on + strand at 5217.090 kb on + strand at 5217.139 kb on + strand at 5217.139 kb on + strand at 5217.139 kb on + strand at 5217.140 kb on - strand at 5217.140 kb on - strand at 5217.140 kb on - strand at 5217.140 kb on - strand at 5217.140 kb on - strand at 5217.142 kb on + strand at 5217.143 kb on - strand at 5217.267 kb on + strand at 5217.267 kb on + strand at 5217.268 kb on - strand at 5217.268 kb on - strand at 5217.279 kb on + strand at 5217.279 kb on + strand at 5217.280 kb on - strand at 5217.454 kb on - strand at 5217.454 kb on - strand at 5217.496 kb on + strand at 5217.497 kb on - strand at 5217.517 kb on + strand at 5217.529 kb on + strand at 5217.558 kb on + strand at 5217.558 kb on + strand at 5217.601 kb on + strand at 5217.602 kb on - strand at 5217.651 kb on + strand at 5217.652 kb on - strand at 5217.652 kb on - strand at 5217.674 kb on + strand, within AO353_23780 at 5217.674 kb on + strand, within AO353_23780 at 5217.675 kb on - strand, within AO353_23780 at 5217.675 kb on - strand, within AO353_23780 at 5217.675 kb on - strand, within AO353_23780 at 5217.675 kb on - strand, within AO353_23780 at 5217.675 kb on - strand, within AO353_23780 at 5217.946 kb on + strand, within AO353_23780 at 5217.947 kb on - strand, within AO353_23780 at 5218.080 kb on + strand, within AO353_23780 at 5218.200 kb on + strand, within AO353_23780 at 5218.200 kb on + strand, within AO353_23780 at 5218.200 kb on + strand, within AO353_23780 at 5218.201 kb on - strand, within AO353_23780 at 5218.201 kb on - strand, within AO353_23780 at 5218.201 kb on - strand, within AO353_23780 at 5218.404 kb on + strand, within AO353_23780 at 5218.404 kb on + strand, within AO353_23780 at 5218.405 kb on - strand, within AO353_23780 at 5218.405 kb on - strand, within AO353_23780 at 5218.413 kb on - strand, within AO353_23780 at 5218.545 kb on + strand at 5218.545 kb on + strand at 5218.546 kb on - strand at 5218.546 kb on - strand at 5218.546 kb on - strand at 5218.564 kb on + strand at 5218.564 kb on + strand at 5218.577 kb on - strand at 5218.587 kb on + strand at 5218.651 kb on + strand at 5218.780 kb on + strand, within AO353_23785 at 5218.789 kb on + strand, within AO353_23785 at 5218.790 kb on - strand, within AO353_23785 at 5219.023 kb on - strand at 5219.032 kb on + strand at 5219.032 kb on + strand at 5219.033 kb on - strand at 5219.109 kb on + strand, within AO353_23790 at 5219.109 kb on + strand, within AO353_23790 at 5219.109 kb on + strand at 5219.109 kb on + strand, within AO353_23790 at 5219.158 kb on + strand, within AO353_23790 at 5219.493 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 5,216,606 + AO353_23775 0.75 +0.0 5,216,606 + AO353_23775 0.75 -0.6 5,216,607 - +2.9 5,216,630 + AO353_23775 0.76 +0.1 5,216,630 + AO353_23775 0.76 -0.0 5,216,630 + AO353_23775 0.76 -0.0 5,216,630 + AO353_23775 0.76 +1.2 5,216,630 + AO353_23775 0.76 +0.3 5,216,630 + AO353_23775 0.76 -0.7 5,216,630 + AO353_23775 0.76 -0.6 5,216,630 + AO353_23775 0.76 -0.5 5,216,630 + AO353_23775 0.76 +0.2 5,216,631 - AO353_23775 0.76 +0.4 5,216,631 - AO353_23775 0.76 -1.9 5,216,631 - +1.4 5,216,986 + +0.5 5,216,986 + -1.2 5,217,019 + +0.2 5,217,019 + -1.3 5,217,090 + -0.7 5,217,139 + +0.4 5,217,139 + +2.4 5,217,139 + +1.9 5,217,140 - -2.1 5,217,140 - -2.7 5,217,140 - -1.2 5,217,140 - -0.9 5,217,140 - -1.1 5,217,142 + +1.6 5,217,143 - -0.0 5,217,267 + -0.3 5,217,267 + +0.4 5,217,268 - -0.5 5,217,268 - +0.2 5,217,279 + +0.3 5,217,279 + -2.1 5,217,280 - +0.1 5,217,454 - -0.5 5,217,454 - +0.2 5,217,496 + +1.0 5,217,497 - +0.7 5,217,517 + -1.1 5,217,529 + -0.2 5,217,558 + +0.7 5,217,558 + +0.1 5,217,601 + +0.3 5,217,602 - -0.9 5,217,651 + +2.0 5,217,652 - +0.1 5,217,652 - +1.0 5,217,674 + AO353_23780 0.12 -0.9 5,217,674 + AO353_23780 0.12 -0.7 5,217,675 - AO353_23780 0.12 -1.4 5,217,675 - AO353_23780 0.12 +3.0 5,217,675 - AO353_23780 0.12 -1.5 5,217,675 - AO353_23780 0.12 -0.3 5,217,675 - AO353_23780 0.12 -1.8 5,217,946 + AO353_23780 0.41 -1.6 5,217,947 - AO353_23780 0.41 -0.2 5,218,080 + AO353_23780 0.55 +0.8 5,218,200 + AO353_23780 0.67 +0.7 5,218,200 + AO353_23780 0.67 -1.4 5,218,200 + AO353_23780 0.67 +0.1 5,218,201 - AO353_23780 0.68 -0.8 5,218,201 - AO353_23780 0.68 -0.5 5,218,201 - AO353_23780 0.68 -1.8 5,218,404 + AO353_23780 0.89 -0.0 5,218,404 + AO353_23780 0.89 +1.6 5,218,405 - AO353_23780 0.89 -0.3 5,218,405 - AO353_23780 0.89 +1.1 5,218,413 - AO353_23780 0.90 +0.3 5,218,545 + -0.8 5,218,545 + +0.6 5,218,546 - +0.2 5,218,546 - -0.4 5,218,546 - +0.7 5,218,564 + -0.9 5,218,564 + -0.1 5,218,577 - +0.4 5,218,587 + -0.5 5,218,651 + +0.6 5,218,780 + AO353_23785 0.39 -2.1 5,218,789 + AO353_23785 0.41 -1.3 5,218,790 - AO353_23785 0.41 +1.1 5,219,023 - -0.2 5,219,032 + +2.0 5,219,032 + -0.8 5,219,033 - +1.2 5,219,109 + AO353_23790 0.14 +0.8 5,219,109 + AO353_23790 0.14 -1.4 5,219,109 + -0.9 5,219,109 + AO353_23790 0.14 -1.4 5,219,158 + AO353_23790 0.25 +1.4 5,219,493 + +1.2
Or see this region's nucleotide sequence