Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_05585 and AO353_05590 are separated by 4 nucleotides AO353_05590 and AO353_05595 are separated by 66 nucleotides AO353_05595 and AO353_05600 are separated by 16 nucleotides AO353_05600 and AO353_05605 are separated by 8 nucleotides
AO353_05585: AO353_05585 - preprotein translocase subunit SecG, at 1,234,293 to 1,234,676
_05585
AO353_05590: AO353_05590 - triosephosphate isomerase, at 1,234,681 to 1,235,436
_05590
AO353_05595: AO353_05595 - phosphoglucosamine mutase, at 1,235,503 to 1,236,840
_05595
AO353_05600: AO353_05600 - dihydropteroate synthase, at 1,236,857 to 1,237,708
_05600
AO353_05605: AO353_05605 - ATP-dependent metalloprotease, at 1,237,717 to 1,239,618
_05605
Position (kb)
1235
1236
1237 Strain fitness (log2 ratio)
-2
-1
0
1 at 1235.425 kb on - strand at 1235.442 kb on - strand at 1235.442 kb on - strand at 1235.488 kb on - strand at 1235.488 kb on - strand at 1236.841 kb on - strand at 1236.841 kb on - strand at 1236.841 kb on - strand at 1236.859 kb on - strand at 1237.698 kb on - strand at 1237.698 kb on - strand at 1237.698 kb on - strand at 1237.698 kb on - strand at 1237.698 kb on - strand at 1237.698 kb on - strand at 1237.698 kb on - strand at 1237.719 kb on - strand at 1237.719 kb on - strand at 1237.719 kb on - strand at 1237.719 kb on - strand at 1237.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 1,235,425 - +0.9 1,235,442 - -1.2 1,235,442 - -0.3 1,235,488 - +0.5 1,235,488 - +1.1 1,236,841 - +0.8 1,236,841 - -0.5 1,236,841 - -0.1 1,236,859 - +0.2 1,237,698 - -1.6 1,237,698 - -1.8 1,237,698 - +1.1 1,237,698 - +0.3 1,237,698 - -0.6 1,237,698 - -1.4 1,237,698 - -0.5 1,237,719 - +0.2 1,237,719 - +0.1 1,237,719 - -0.2 1,237,719 - -0.1 1,237,719 - -0.3
Or see this region's nucleotide sequence