Experiment: Fusidic 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_01405 and AO353_01410 are separated by 740 nucleotides AO353_01410 and AO353_01415 are separated by 198 nucleotides AO353_01415 and AO353_01420 overlap by 1 nucleotides
AO353_01405: AO353_01405 - 23S rRNA pseudouridylate synthase, at 299,997 to 300,956
_01405
AO353_01410: AO353_01410 - ribonuclease E, at 301,697 to 304,882
_01410
AO353_01415: AO353_01415 - molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 305,081 to 305,686
_01415
AO353_01420: AO353_01420 - acylaldehyde oxidase, at 305,686 to 308,001
_01420
Position (kb) 301
302
303
304
305 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 300.776 kb on - strand, within AO353_01405 at 300.878 kb on + strand at 300.879 kb on - strand at 300.879 kb on - strand at 300.884 kb on + strand at 300.885 kb on - strand at 300.885 kb on - strand at 300.898 kb on + strand at 300.899 kb on - strand at 300.899 kb on - strand at 300.924 kb on + strand at 300.924 kb on + strand at 300.924 kb on + strand at 300.924 kb on + strand at 300.924 kb on + strand at 300.925 kb on - strand at 300.925 kb on - strand at 300.925 kb on - strand at 300.925 kb on - strand at 300.925 kb on - strand at 300.925 kb on - strand at 300.925 kb on - strand at 300.996 kb on + strand at 301.009 kb on + strand at 301.142 kb on + strand at 301.142 kb on + strand at 301.142 kb on + strand at 301.189 kb on + strand at 301.212 kb on + strand at 301.216 kb on + strand at 301.216 kb on + strand at 301.287 kb on + strand at 301.287 kb on + strand at 301.330 kb on + strand at 301.366 kb on + strand at 301.366 kb on + strand at 301.366 kb on + strand at 301.366 kb on + strand at 304.335 kb on + strand, within AO353_01410 at 304.347 kb on + strand, within AO353_01410 at 304.347 kb on + strand, within AO353_01410 at 304.347 kb on + strand, within AO353_01410 at 304.347 kb on + strand, within AO353_01410 at 304.348 kb on - strand, within AO353_01410 at 304.933 kb on + strand at 304.992 kb on + strand at 305.000 kb on + strand at 305.000 kb on + strand at 305.001 kb on - strand at 305.001 kb on - strand at 305.001 kb on - strand at 305.001 kb on - strand at 305.038 kb on + strand at 305.039 kb on - strand at 305.039 kb on - strand at 305.039 kb on - strand at 305.052 kb on - strand at 305.082 kb on + strand at 305.082 kb on + strand at 305.082 kb on + strand at 305.082 kb on + strand at 305.082 kb on + strand at 305.083 kb on - strand at 305.138 kb on + strand at 305.314 kb on + strand, within AO353_01415 at 305.314 kb on + strand, within AO353_01415 at 305.314 kb on + strand, within AO353_01415 at 305.329 kb on + strand, within AO353_01415 at 305.330 kb on - strand, within AO353_01415 at 305.330 kb on - strand, within AO353_01415 at 305.330 kb on - strand, within AO353_01415 at 305.330 kb on - strand, within AO353_01415 at 305.338 kb on + strand, within AO353_01415 at 305.445 kb on + strand, within AO353_01415 at 305.445 kb on + strand, within AO353_01415 at 305.446 kb on - strand, within AO353_01415 at 305.446 kb on - strand, within AO353_01415 at 305.446 kb on - strand, within AO353_01415 at 305.446 kb on - strand, within AO353_01415 at 305.446 kb on - strand, within AO353_01415 at 305.446 kb on - strand, within AO353_01415 at 305.742 kb on + strand at 305.742 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.125 mg/ml remove 300,776 - AO353_01405 0.81 +0.2 300,878 + +1.5 300,879 - +0.5 300,879 - +0.2 300,884 + -0.2 300,885 - +2.6 300,885 - -1.2 300,898 + +1.2 300,899 - +1.6 300,899 - +1.4 300,924 + +0.6 300,924 + +0.5 300,924 + +1.4 300,924 + +2.2 300,924 + +0.7 300,925 - -0.8 300,925 - +0.1 300,925 - +0.2 300,925 - -0.1 300,925 - +0.2 300,925 - +0.6 300,925 - +0.9 300,996 + +0.2 301,009 + +2.1 301,142 + -1.1 301,142 + -0.2 301,142 + -0.7 301,189 + -0.4 301,212 + -0.1 301,216 + -0.9 301,216 + +0.8 301,287 + +0.3 301,287 + +0.3 301,330 + +0.6 301,366 + -0.5 301,366 + +0.0 301,366 + +1.4 301,366 + +1.2 304,335 + AO353_01410 0.83 -3.1 304,347 + AO353_01410 0.83 -1.8 304,347 + AO353_01410 0.83 -1.2 304,347 + AO353_01410 0.83 -1.6 304,347 + AO353_01410 0.83 -0.6 304,348 - AO353_01410 0.83 -1.8 304,933 + +0.9 304,992 + +0.1 305,000 + +0.5 305,000 + -1.1 305,001 - +3.3 305,001 - -0.5 305,001 - -0.6 305,001 - -1.1 305,038 + +0.2 305,039 - -0.1 305,039 - +1.8 305,039 - -1.2 305,052 - -2.3 305,082 + +2.0 305,082 + +2.1 305,082 + +0.4 305,082 + +1.2 305,082 + +0.8 305,083 - +0.1 305,138 + -1.7 305,314 + AO353_01415 0.38 -1.6 305,314 + AO353_01415 0.38 +0.1 305,314 + AO353_01415 0.38 +0.2 305,329 + AO353_01415 0.41 -1.1 305,330 - AO353_01415 0.41 +0.0 305,330 - AO353_01415 0.41 +1.3 305,330 - AO353_01415 0.41 +1.0 305,330 - AO353_01415 0.41 -1.8 305,338 + AO353_01415 0.42 -2.2 305,445 + AO353_01415 0.60 -0.1 305,445 + AO353_01415 0.60 -0.0 305,446 - AO353_01415 0.60 +0.2 305,446 - AO353_01415 0.60 +0.2 305,446 - AO353_01415 0.60 +0.2 305,446 - AO353_01415 0.60 +0.5 305,446 - AO353_01415 0.60 +1.8 305,446 - AO353_01415 0.60 -1.0 305,742 + -0.2 305,742 + -0.2
Or see this region's nucleotide sequence