Experiment: D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS13220 and ABZR86_RS13225 are separated by 60 nucleotides ABZR86_RS13225 and ABZR86_RS13230 are separated by 17 nucleotides ABZR86_RS13230 and ABZR86_RS13235 are separated by 84 nucleotides ABZR86_RS13235 and ABZR86_RS13240 are separated by 70 nucleotides
ABZR86_RS13220: ABZR86_RS13220 - AraC family transcriptional regulator, at 2,969,499 to 2,970,410
_RS13220
ABZR86_RS13225: ABZR86_RS13225 - HAD family hydrolase, at 2,970,471 to 2,971,160
_RS13225
ABZR86_RS13230: ABZR86_RS13230 - DUF4440 domain-containing protein, at 2,971,178 to 2,971,564
_RS13230
ABZR86_RS13235: ABZR86_RS13235 - hypothetical protein, at 2,971,649 to 2,971,978
_RS13235
ABZR86_RS13240: ABZR86_RS13240 - class I SAM-dependent methyltransferase, at 2,972,049 to 2,972,666
_RS13240
Position (kb)
2970
2971
2972 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2969.498 kb on + strand at 2969.498 kb on + strand at 2969.498 kb on + strand at 2969.498 kb on + strand at 2969.502 kb on + strand at 2969.502 kb on + strand at 2969.502 kb on + strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.503 kb on - strand at 2969.504 kb on + strand at 2969.646 kb on + strand, within ABZR86_RS13220 at 2969.646 kb on + strand, within ABZR86_RS13220 at 2969.647 kb on - strand, within ABZR86_RS13220 at 2969.823 kb on + strand, within ABZR86_RS13220 at 2969.824 kb on - strand, within ABZR86_RS13220 at 2969.824 kb on - strand, within ABZR86_RS13220 at 2970.018 kb on + strand, within ABZR86_RS13220 at 2970.213 kb on + strand, within ABZR86_RS13220 at 2970.214 kb on - strand, within ABZR86_RS13220 at 2970.222 kb on + strand, within ABZR86_RS13220 at 2970.223 kb on - strand, within ABZR86_RS13220 at 2970.321 kb on + strand at 2970.321 kb on + strand at 2970.322 kb on - strand at 2970.338 kb on + strand at 2970.339 kb on - strand at 2970.406 kb on + strand at 2970.406 kb on + strand at 2970.407 kb on - strand at 2970.644 kb on + strand, within ABZR86_RS13225 at 2970.783 kb on + strand, within ABZR86_RS13225 at 2970.783 kb on + strand, within ABZR86_RS13225 at 2971.328 kb on + strand, within ABZR86_RS13230 at 2971.340 kb on + strand, within ABZR86_RS13230 at 2971.340 kb on + strand, within ABZR86_RS13230 at 2971.340 kb on + strand, within ABZR86_RS13230 at 2971.341 kb on - strand, within ABZR86_RS13230 at 2971.341 kb on - strand, within ABZR86_RS13230 at 2971.341 kb on - strand, within ABZR86_RS13230 at 2971.341 kb on - strand, within ABZR86_RS13230 at 2971.439 kb on + strand, within ABZR86_RS13230 at 2971.439 kb on + strand, within ABZR86_RS13230 at 2971.439 kb on + strand, within ABZR86_RS13230 at 2971.439 kb on + strand, within ABZR86_RS13230 at 2971.451 kb on + strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.452 kb on - strand, within ABZR86_RS13230 at 2971.633 kb on + strand at 2971.634 kb on - strand at 2971.641 kb on + strand at 2971.641 kb on + strand at 2971.641 kb on + strand at 2971.642 kb on - strand at 2971.642 kb on - strand at 2971.796 kb on + strand, within ABZR86_RS13235 at 2971.796 kb on + strand, within ABZR86_RS13235 at 2971.797 kb on - strand, within ABZR86_RS13235 at 2971.797 kb on - strand, within ABZR86_RS13235 at 2971.982 kb on - strand at 2971.982 kb on - strand at 2971.996 kb on + strand at 2971.997 kb on - strand at 2971.997 kb on - strand at 2972.141 kb on - strand, within ABZR86_RS13240
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml remove 2,969,498 + +0.1 2,969,498 + +0.2 2,969,498 + +0.2 2,969,498 + +0.2 2,969,502 + -0.0 2,969,502 + -1.1 2,969,502 + -0.0 2,969,503 - -0.1 2,969,503 - +0.1 2,969,503 - -0.1 2,969,503 - -0.7 2,969,503 - +0.1 2,969,503 - -0.1 2,969,503 - +0.5 2,969,503 - +0.1 2,969,503 - +0.5 2,969,503 - +0.2 2,969,504 + +0.2 2,969,646 + ABZR86_RS13220 0.16 -0.1 2,969,646 + ABZR86_RS13220 0.16 +0.2 2,969,647 - ABZR86_RS13220 0.16 +0.1 2,969,823 + ABZR86_RS13220 0.36 +1.2 2,969,824 - ABZR86_RS13220 0.36 +0.3 2,969,824 - ABZR86_RS13220 0.36 +0.5 2,970,018 + ABZR86_RS13220 0.57 +0.2 2,970,213 + ABZR86_RS13220 0.78 -0.1 2,970,214 - ABZR86_RS13220 0.78 +0.7 2,970,222 + ABZR86_RS13220 0.79 +0.0 2,970,223 - ABZR86_RS13220 0.79 -0.1 2,970,321 + +2.9 2,970,321 + +0.1 2,970,322 - +0.0 2,970,338 + -0.3 2,970,339 - +0.7 2,970,406 + -0.3 2,970,406 + +0.7 2,970,407 - +1.3 2,970,644 + ABZR86_RS13225 0.25 -0.1 2,970,783 + ABZR86_RS13225 0.45 +0.7 2,970,783 + ABZR86_RS13225 0.45 +0.6 2,971,328 + ABZR86_RS13230 0.39 +0.2 2,971,340 + ABZR86_RS13230 0.42 +0.8 2,971,340 + ABZR86_RS13230 0.42 -0.1 2,971,340 + ABZR86_RS13230 0.42 -0.0 2,971,341 - ABZR86_RS13230 0.42 -0.2 2,971,341 - ABZR86_RS13230 0.42 -0.0 2,971,341 - ABZR86_RS13230 0.42 +0.1 2,971,341 - ABZR86_RS13230 0.42 +0.3 2,971,439 + ABZR86_RS13230 0.67 -0.1 2,971,439 + ABZR86_RS13230 0.67 -0.1 2,971,439 + ABZR86_RS13230 0.67 +0.4 2,971,439 + ABZR86_RS13230 0.67 +0.4 2,971,451 + ABZR86_RS13230 0.71 -0.1 2,971,452 - ABZR86_RS13230 0.71 -0.4 2,971,452 - ABZR86_RS13230 0.71 -0.1 2,971,452 - ABZR86_RS13230 0.71 +0.0 2,971,452 - ABZR86_RS13230 0.71 +0.9 2,971,452 - ABZR86_RS13230 0.71 -0.0 2,971,452 - ABZR86_RS13230 0.71 +0.0 2,971,633 + +0.1 2,971,634 - +0.3 2,971,641 + +0.1 2,971,641 + +0.5 2,971,641 + +0.0 2,971,642 - -0.1 2,971,642 - -0.4 2,971,796 + ABZR86_RS13235 0.45 -0.3 2,971,796 + ABZR86_RS13235 0.45 +0.0 2,971,797 - ABZR86_RS13235 0.45 -0.1 2,971,797 - ABZR86_RS13235 0.45 -0.2 2,971,982 - -0.6 2,971,982 - +0.4 2,971,996 + +0.0 2,971,997 - -0.6 2,971,997 - +0.2 2,972,141 - ABZR86_RS13240 0.15 +0.3
Or see this region's nucleotide sequence