Experiment: D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS03230 and ABZR86_RS03235 are separated by 19 nucleotides ABZR86_RS03235 and ABZR86_RS03240 are separated by 19 nucleotides ABZR86_RS03240 and ABZR86_RS03245 are separated by 77 nucleotides ABZR86_RS03245 and ABZR86_RS03250 are separated by 271 nucleotides ABZR86_RS03250 and ABZR86_RS03255 are separated by 17 nucleotides
ABZR86_RS03230: ABZR86_RS03230 - ornithine cyclodeaminase family protein, at 674,897 to 675,862
_RS03230
ABZR86_RS03235: ABZR86_RS03235 - cytochrome P460 family protein, at 675,882 to 676,391
_RS03235
ABZR86_RS03240: ABZR86_RS03240 - alpha/beta hydrolase, at 676,411 to 677,472
_RS03240
ABZR86_RS03245: ABZR86_RS03245 - hypothetical protein, at 677,550 to 677,846
_RS03245
ABZR86_RS03250: ABZR86_RS03250 - type II toxin-antitoxin system RelE/ParE family toxin, at 678,118 to 678,408
_RS03250
ABZR86_RS03255: ABZR86_RS03255 - addiction module antidote protein, at 678,426 to 678,830
_RS03255
Position (kb)
676
677
678 Strain fitness (log2 ratio)
-1
0
1 at 675.635 kb on + strand, within ABZR86_RS03230 at 675.635 kb on + strand, within ABZR86_RS03230 at 675.636 kb on - strand, within ABZR86_RS03230 at 675.636 kb on - strand, within ABZR86_RS03230 at 675.818 kb on + strand at 675.819 kb on - strand at 675.840 kb on - strand at 675.851 kb on + strand at 675.851 kb on + strand at 675.893 kb on - strand at 675.943 kb on + strand, within ABZR86_RS03235 at 675.944 kb on - strand, within ABZR86_RS03235 at 675.944 kb on - strand, within ABZR86_RS03235 at 675.944 kb on - strand, within ABZR86_RS03235 at 675.998 kb on - strand, within ABZR86_RS03235 at 676.021 kb on + strand, within ABZR86_RS03235 at 676.021 kb on + strand, within ABZR86_RS03235 at 676.022 kb on - strand, within ABZR86_RS03235 at 676.282 kb on + strand, within ABZR86_RS03235 at 676.283 kb on - strand, within ABZR86_RS03235 at 676.385 kb on + strand at 676.385 kb on + strand at 676.385 kb on + strand at 676.433 kb on - strand at 676.508 kb on + strand at 676.508 kb on + strand at 676.509 kb on - strand at 676.509 kb on - strand at 676.676 kb on + strand, within ABZR86_RS03240 at 676.806 kb on - strand, within ABZR86_RS03240 at 676.815 kb on - strand, within ABZR86_RS03240 at 676.853 kb on + strand, within ABZR86_RS03240 at 676.853 kb on + strand, within ABZR86_RS03240 at 676.854 kb on - strand, within ABZR86_RS03240 at 676.919 kb on + strand, within ABZR86_RS03240 at 677.132 kb on + strand, within ABZR86_RS03240 at 677.186 kb on + strand, within ABZR86_RS03240 at 677.187 kb on - strand, within ABZR86_RS03240 at 677.192 kb on + strand, within ABZR86_RS03240 at 677.192 kb on + strand, within ABZR86_RS03240 at 677.192 kb on + strand, within ABZR86_RS03240 at 677.193 kb on - strand, within ABZR86_RS03240 at 677.193 kb on - strand, within ABZR86_RS03240 at 677.193 kb on - strand, within ABZR86_RS03240 at 677.193 kb on - strand, within ABZR86_RS03240 at 677.193 kb on - strand, within ABZR86_RS03240 at 677.193 kb on - strand, within ABZR86_RS03240 at 677.202 kb on - strand, within ABZR86_RS03240 at 677.861 kb on + strand at 677.925 kb on + strand at 677.978 kb on + strand at 677.979 kb on - strand at 677.979 kb on - strand at 677.979 kb on - strand at 678.244 kb on + strand, within ABZR86_RS03250 at 678.244 kb on + strand, within ABZR86_RS03250 at 678.298 kb on + strand, within ABZR86_RS03250 at 678.299 kb on - strand, within ABZR86_RS03250 at 678.299 kb on - strand, within ABZR86_RS03250 at 678.325 kb on + strand, within ABZR86_RS03250 at 678.326 kb on - strand, within ABZR86_RS03250
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.032 mg/ml remove 675,635 + ABZR86_RS03230 0.76 +0.5 675,635 + ABZR86_RS03230 0.76 -0.4 675,636 - ABZR86_RS03230 0.77 -0.1 675,636 - ABZR86_RS03230 0.77 -0.0 675,818 + -0.3 675,819 - -0.7 675,840 - +0.0 675,851 + -0.6 675,851 + +0.0 675,893 - -0.1 675,943 + ABZR86_RS03235 0.12 +0.2 675,944 - ABZR86_RS03235 0.12 -0.0 675,944 - ABZR86_RS03235 0.12 +0.6 675,944 - ABZR86_RS03235 0.12 +0.3 675,998 - ABZR86_RS03235 0.23 -1.0 676,021 + ABZR86_RS03235 0.27 +0.4 676,021 + ABZR86_RS03235 0.27 -0.2 676,022 - ABZR86_RS03235 0.27 -0.2 676,282 + ABZR86_RS03235 0.78 +0.3 676,283 - ABZR86_RS03235 0.79 +0.3 676,385 + -0.1 676,385 + -0.1 676,385 + +0.1 676,433 - -0.2 676,508 + -0.1 676,508 + +0.1 676,509 - -0.4 676,509 - -0.1 676,676 + ABZR86_RS03240 0.25 -0.4 676,806 - ABZR86_RS03240 0.37 -0.1 676,815 - ABZR86_RS03240 0.38 -0.3 676,853 + ABZR86_RS03240 0.42 -0.4 676,853 + ABZR86_RS03240 0.42 +0.1 676,854 - ABZR86_RS03240 0.42 +0.1 676,919 + ABZR86_RS03240 0.48 +0.1 677,132 + ABZR86_RS03240 0.68 -0.4 677,186 + ABZR86_RS03240 0.73 -0.4 677,187 - ABZR86_RS03240 0.73 +1.1 677,192 + ABZR86_RS03240 0.74 -0.2 677,192 + ABZR86_RS03240 0.74 +0.5 677,192 + ABZR86_RS03240 0.74 -1.5 677,193 - ABZR86_RS03240 0.74 -0.1 677,193 - ABZR86_RS03240 0.74 -0.3 677,193 - ABZR86_RS03240 0.74 -0.9 677,193 - ABZR86_RS03240 0.74 +0.5 677,193 - ABZR86_RS03240 0.74 -0.4 677,193 - ABZR86_RS03240 0.74 -0.2 677,202 - ABZR86_RS03240 0.74 -1.1 677,861 + +0.9 677,925 + +0.3 677,978 + -0.6 677,979 - +0.0 677,979 - -0.4 677,979 - -0.4 678,244 + ABZR86_RS03250 0.43 -0.8 678,244 + ABZR86_RS03250 0.43 -0.2 678,298 + ABZR86_RS03250 0.62 -0.3 678,299 - ABZR86_RS03250 0.62 +0.1 678,299 - ABZR86_RS03250 0.62 +0.2 678,325 + ABZR86_RS03250 0.71 -0.9 678,326 - ABZR86_RS03250 0.71 +0.7
Or see this region's nucleotide sequence