Experiment: MoYLS4 with Cephalothin 1.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1298 and DvMF_1299 overlap by 4 nucleotides DvMF_1299 and DvMF_1300 overlap by 1 nucleotides DvMF_1300 and DvMF_1301 overlap by 14 nucleotides
DvMF_1298: DvMF_1298 - phage tail tape measure protein, TP901 family (RefSeq), at 1,587,062 to 1,589,407
_1298
DvMF_1299: DvMF_1299 - hypothetical protein (RefSeq), at 1,589,404 to 1,589,949
_1299
DvMF_1300: DvMF_1300 - hypothetical protein (RefSeq), at 1,589,949 to 1,590,749
_1300
DvMF_1301: DvMF_1301 - baseplate assembly protein, putative (RefSeq), at 1,590,736 to 1,591,461
_1301
Position (kb)
1589
1590
1591 Strain fitness (log2 ratio)
-1
0
1 at 1589.009 kb on - strand, within DvMF_1298 at 1589.131 kb on - strand, within DvMF_1298 at 1589.190 kb on - strand at 1589.226 kb on - strand at 1589.316 kb on + strand at 1589.417 kb on + strand at 1589.579 kb on + strand, within DvMF_1299 at 1589.587 kb on - strand, within DvMF_1299 at 1589.622 kb on + strand, within DvMF_1299 at 1589.854 kb on + strand, within DvMF_1299 at 1589.879 kb on - strand, within DvMF_1299 at 1590.058 kb on + strand, within DvMF_1300 at 1590.254 kb on + strand, within DvMF_1300 at 1590.462 kb on - strand, within DvMF_1300 at 1590.507 kb on + strand, within DvMF_1300 at 1590.509 kb on + strand, within DvMF_1300 at 1590.929 kb on + strand, within DvMF_1301 at 1591.014 kb on - strand, within DvMF_1301 at 1591.571 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Cephalothin 1.25 mg/ml remove 1,589,009 - DvMF_1298 0.83 -0.3 1,589,131 - DvMF_1298 0.88 +0.2 1,589,190 - -0.5 1,589,226 - -0.2 1,589,316 + -0.2 1,589,417 + +0.0 1,589,579 + DvMF_1299 0.32 -0.5 1,589,587 - DvMF_1299 0.34 +0.1 1,589,622 + DvMF_1299 0.40 -0.5 1,589,854 + DvMF_1299 0.82 -0.9 1,589,879 - DvMF_1299 0.87 -0.4 1,590,058 + DvMF_1300 0.14 -0.2 1,590,254 + DvMF_1300 0.38 -0.2 1,590,462 - DvMF_1300 0.64 -0.2 1,590,507 + DvMF_1300 0.70 -0.1 1,590,509 + DvMF_1300 0.70 -0.2 1,590,929 + DvMF_1301 0.27 -0.2 1,591,014 - DvMF_1301 0.38 -0.0 1,591,571 - -0.2
Or see this region's nucleotide sequence