Experiment: MoYLS4 with Cephalothin 1.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0603 and DvMF_0604 are separated by 7 nucleotides DvMF_0604 and DvMF_0605 overlap by 4 nucleotides DvMF_0605 and DvMF_0606 are separated by 214 nucleotides
DvMF_0603: DvMF_0603 - 2-oxoglutarate ferredoxin oxidoreductase subunit alpha (RefSeq), at 715,557 to 716,705
_0603
DvMF_0604: DvMF_0604 - 2-oxoglutarate ferredoxin oxidoreductase subunit beta (RefSeq), at 716,713 to 717,546
_0604
DvMF_0605: DvMF_0605 - pyruvate ferredoxin/flavodoxin oxidoreductase (RefSeq), at 717,543 to 718,166
_0605
DvMF_0606: DvMF_0606 - hypothetical protein (RefSeq), at 718,381 to 718,677
_0606
Position (kb) 716
717
718 Strain fitness (log2 ratio) -1
0
1 at 715.985 kb on + strand, within DvMF_0603 at 716.127 kb on - strand, within DvMF_0603 at 716.697 kb on + strand at 716.918 kb on - strand, within DvMF_0604 at 717.096 kb on + strand, within DvMF_0604 at 717.096 kb on + strand, within DvMF_0604 at 717.128 kb on - strand, within DvMF_0604 at 717.204 kb on + strand, within DvMF_0604 at 717.204 kb on + strand, within DvMF_0604 at 717.332 kb on - strand, within DvMF_0604 at 717.401 kb on - strand, within DvMF_0604 at 717.843 kb on + strand, within DvMF_0605 at 717.857 kb on + strand, within DvMF_0605 at 717.980 kb on - strand, within DvMF_0605 at 718.057 kb on + strand, within DvMF_0605 at 718.169 kb on - strand at 718.235 kb on + strand at 718.372 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Cephalothin 1.25 mg/ml remove 715,985 + DvMF_0603 0.37 -0.8 716,127 - DvMF_0603 0.50 -0.6 716,697 + -0.3 716,918 - DvMF_0604 0.25 -1.0 717,096 + DvMF_0604 0.46 -0.9 717,096 + DvMF_0604 0.46 -0.2 717,128 - DvMF_0604 0.50 -0.7 717,204 + DvMF_0604 0.59 -0.3 717,204 + DvMF_0604 0.59 -0.6 717,332 - DvMF_0604 0.74 -0.7 717,401 - DvMF_0604 0.82 -0.5 717,843 + DvMF_0605 0.48 +0.4 717,857 + DvMF_0605 0.50 -0.4 717,980 - DvMF_0605 0.70 -0.4 718,057 + DvMF_0605 0.82 -0.6 718,169 - +0.0 718,235 + -0.2 718,372 + -0.5
Or see this region's nucleotide sequence