Experiment: PYE with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02149 and CCNA_02150 are separated by 17 nucleotides CCNA_02150 and CCNA_02151 are separated by 37 nucleotides CCNA_02151 and CCNA_02152 are separated by 97 nucleotides
CCNA_02149: CCNA_02149 - 1-deoxy-D-xylulose 5-phosphate synthase, at 2,305,334 to 2,307,256
_02149
CCNA_02150: CCNA_02150 - dimethylallyltransferase/geranyltranstransferase , at 2,307,274 to 2,308,158
_02150
CCNA_02151: CCNA_02151 - exodeoxyribonuclease VII small subunit, at 2,308,196 to 2,308,450
_02151
CCNA_02152: CCNA_02152 - phosphoglycerate mutase, at 2,308,548 to 2,309,207
_02152
Position (kb)
2307
2308
2309 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2308.187 kb on + strand at 2308.245 kb on - strand, within CCNA_02151 at 2308.265 kb on - strand, within CCNA_02151 at 2308.280 kb on - strand, within CCNA_02151 at 2308.342 kb on + strand, within CCNA_02151 at 2308.345 kb on - strand, within CCNA_02151 at 2308.345 kb on - strand, within CCNA_02151 at 2308.345 kb on - strand, within CCNA_02151 at 2308.357 kb on + strand, within CCNA_02151 at 2308.385 kb on - strand, within CCNA_02151 at 2308.421 kb on - strand, within CCNA_02151 at 2308.448 kb on - strand at 2308.472 kb on + strand at 2308.478 kb on - strand at 2308.485 kb on - strand at 2308.507 kb on + strand at 2308.515 kb on - strand at 2308.668 kb on - strand, within CCNA_02152 at 2308.772 kb on - strand, within CCNA_02152 at 2308.788 kb on - strand, within CCNA_02152 at 2308.788 kb on - strand, within CCNA_02152 at 2308.877 kb on - strand, within CCNA_02152 at 2308.887 kb on - strand, within CCNA_02152 at 2308.952 kb on + strand, within CCNA_02152 at 2308.977 kb on - strand, within CCNA_02152 at 2309.000 kb on - strand, within CCNA_02152 at 2309.000 kb on - strand, within CCNA_02152 at 2309.010 kb on - strand, within CCNA_02152 at 2309.010 kb on - strand, within CCNA_02152 at 2309.010 kb on - strand, within CCNA_02152 at 2309.011 kb on - strand, within CCNA_02152 at 2309.046 kb on - strand, within CCNA_02152 at 2309.051 kb on - strand, within CCNA_02152 at 2309.066 kb on - strand, within CCNA_02152 at 2309.086 kb on - strand, within CCNA_02152 at 2309.102 kb on + strand, within CCNA_02152 at 2309.133 kb on - strand, within CCNA_02152 at 2309.133 kb on - strand, within CCNA_02152
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.025 mg/ml remove 2,308,187 + +0.3 2,308,245 - CCNA_02151 0.19 +1.4 2,308,265 - CCNA_02151 0.27 +1.3 2,308,280 - CCNA_02151 0.33 +2.4 2,308,342 + CCNA_02151 0.57 -2.4 2,308,345 - CCNA_02151 0.58 +2.9 2,308,345 - CCNA_02151 0.58 +2.7 2,308,345 - CCNA_02151 0.58 +2.4 2,308,357 + CCNA_02151 0.63 +0.9 2,308,385 - CCNA_02151 0.74 +1.6 2,308,421 - CCNA_02151 0.88 +1.9 2,308,448 - +2.8 2,308,472 + -0.0 2,308,478 - +1.6 2,308,485 - +0.3 2,308,507 + -1.2 2,308,515 - +0.5 2,308,668 - CCNA_02152 0.18 +0.3 2,308,772 - CCNA_02152 0.34 -0.4 2,308,788 - CCNA_02152 0.36 -3.0 2,308,788 - CCNA_02152 0.36 -1.0 2,308,877 - CCNA_02152 0.50 -0.3 2,308,887 - CCNA_02152 0.51 +0.1 2,308,952 + CCNA_02152 0.61 -0.9 2,308,977 - CCNA_02152 0.65 -1.7 2,309,000 - CCNA_02152 0.68 +0.3 2,309,000 - CCNA_02152 0.68 -1.0 2,309,010 - CCNA_02152 0.70 +0.5 2,309,010 - CCNA_02152 0.70 -1.4 2,309,010 - CCNA_02152 0.70 +0.1 2,309,011 - CCNA_02152 0.70 -0.3 2,309,046 - CCNA_02152 0.75 -0.2 2,309,051 - CCNA_02152 0.76 +1.0 2,309,066 - CCNA_02152 0.78 -1.0 2,309,086 - CCNA_02152 0.82 -1.7 2,309,102 + CCNA_02152 0.84 -2.5 2,309,133 - CCNA_02152 0.89 -0.2 2,309,133 - CCNA_02152 0.89 -0.0
Or see this region's nucleotide sequence