Experiment: PYE with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02065 and CCNA_02066 are separated by 134 nucleotides CCNA_02066 and CCNA_02067 are separated by 71 nucleotides CCNA_02067 and CCNA_02068 are separated by 95 nucleotides CCNA_02068 and CCNA_02069 overlap by 1 nucleotides
CCNA_02065: CCNA_02065 - acylamino-acid-releasing enzyme, at 2,215,780 to 2,217,882
_02065
CCNA_02066: CCNA_02066 - phytoene/squalene synthase family protein, at 2,218,017 to 2,218,730
_02066
CCNA_02067: CCNA_02067 - conserved membrane spanning protein, at 2,218,802 to 2,219,416
_02067
CCNA_02068: CCNA_02068 - Mth938-like membrane protein, at 2,219,512 to 2,219,865
_02068
CCNA_02069: CCNA_02069 - protein translocase subunit secF, at 2,219,865 to 2,220,830
_02069
Position (kb)
2218
2219
2220 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2217.802 kb on + strand at 2217.802 kb on + strand at 2217.802 kb on + strand at 2217.832 kb on + strand at 2217.850 kb on - strand at 2217.993 kb on + strand at 2218.023 kb on - strand at 2218.155 kb on - strand, within CCNA_02066 at 2218.168 kb on + strand, within CCNA_02066 at 2218.168 kb on + strand, within CCNA_02066 at 2218.176 kb on - strand, within CCNA_02066 at 2218.176 kb on - strand, within CCNA_02066 at 2218.176 kb on - strand, within CCNA_02066 at 2218.180 kb on + strand, within CCNA_02066 at 2218.215 kb on + strand, within CCNA_02066 at 2218.223 kb on - strand, within CCNA_02066 at 2218.224 kb on - strand, within CCNA_02066 at 2218.295 kb on - strand, within CCNA_02066 at 2218.295 kb on - strand at 2218.402 kb on + strand, within CCNA_02066 at 2218.448 kb on - strand, within CCNA_02066 at 2218.470 kb on + strand, within CCNA_02066 at 2218.483 kb on + strand, within CCNA_02066 at 2218.726 kb on + strand at 2218.771 kb on + strand at 2218.771 kb on + strand at 2218.779 kb on - strand at 2218.807 kb on - strand at 2218.825 kb on + strand at 2218.858 kb on + strand at 2218.863 kb on - strand at 2218.866 kb on - strand at 2218.904 kb on - strand, within CCNA_02067 at 2218.904 kb on - strand, within CCNA_02067 at 2218.904 kb on - strand, within CCNA_02067 at 2218.904 kb on - strand, within CCNA_02067 at 2218.904 kb on - strand, within CCNA_02067 at 2218.910 kb on - strand, within CCNA_02067 at 2218.926 kb on + strand, within CCNA_02067 at 2218.939 kb on - strand, within CCNA_02067 at 2219.015 kb on - strand, within CCNA_02067 at 2219.026 kb on - strand, within CCNA_02067 at 2219.062 kb on - strand, within CCNA_02067 at 2219.153 kb on - strand, within CCNA_02067 at 2219.188 kb on + strand, within CCNA_02067 at 2219.241 kb on + strand, within CCNA_02067 at 2219.246 kb on - strand, within CCNA_02067 at 2219.264 kb on - strand, within CCNA_02067 at 2219.311 kb on - strand, within CCNA_02067 at 2219.323 kb on + strand, within CCNA_02067 at 2219.461 kb on - strand at 2219.546 kb on - strand at 2219.593 kb on + strand, within CCNA_02068 at 2219.619 kb on + strand, within CCNA_02068 at 2219.659 kb on + strand, within CCNA_02068 at 2219.659 kb on + strand, within CCNA_02068 at 2219.659 kb on + strand, within CCNA_02068 at 2219.667 kb on - strand, within CCNA_02068 at 2219.667 kb on - strand, within CCNA_02068 at 2219.667 kb on - strand, within CCNA_02068 at 2219.667 kb on - strand, within CCNA_02068 at 2219.667 kb on - strand, within CCNA_02068 at 2219.754 kb on + strand, within CCNA_02068 at 2219.787 kb on + strand, within CCNA_02068
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.025 mg/ml remove 2,217,802 + +0.7 2,217,802 + +1.0 2,217,802 + +0.5 2,217,832 + -0.0 2,217,850 - -1.7 2,217,993 + +0.3 2,218,023 - +1.1 2,218,155 - CCNA_02066 0.19 +3.5 2,218,168 + CCNA_02066 0.21 +2.3 2,218,168 + CCNA_02066 0.21 +1.7 2,218,176 - CCNA_02066 0.22 +2.7 2,218,176 - CCNA_02066 0.22 +2.9 2,218,176 - CCNA_02066 0.22 +2.0 2,218,180 + CCNA_02066 0.23 +2.3 2,218,215 + CCNA_02066 0.28 +1.0 2,218,223 - CCNA_02066 0.29 +3.0 2,218,224 - CCNA_02066 0.29 +2.1 2,218,295 - CCNA_02066 0.39 +1.3 2,218,295 - +2.1 2,218,402 + CCNA_02066 0.54 +1.9 2,218,448 - CCNA_02066 0.60 +2.4 2,218,470 + CCNA_02066 0.63 +2.6 2,218,483 + CCNA_02066 0.65 +1.7 2,218,726 + +0.8 2,218,771 + -1.3 2,218,771 + -0.1 2,218,779 - +1.9 2,218,807 - +1.6 2,218,825 + +0.6 2,218,858 + +0.1 2,218,863 - +1.2 2,218,866 - +2.1 2,218,904 - CCNA_02067 0.17 +1.2 2,218,904 - CCNA_02067 0.17 +1.6 2,218,904 - CCNA_02067 0.17 +1.2 2,218,904 - CCNA_02067 0.17 +1.6 2,218,904 - CCNA_02067 0.17 +1.5 2,218,910 - CCNA_02067 0.18 +1.4 2,218,926 + CCNA_02067 0.20 -1.2 2,218,939 - CCNA_02067 0.22 +0.9 2,219,015 - CCNA_02067 0.35 +1.1 2,219,026 - CCNA_02067 0.36 +0.4 2,219,062 - CCNA_02067 0.42 +2.1 2,219,153 - CCNA_02067 0.57 +0.6 2,219,188 + CCNA_02067 0.63 +0.3 2,219,241 + CCNA_02067 0.71 +0.1 2,219,246 - CCNA_02067 0.72 +1.8 2,219,264 - CCNA_02067 0.75 +0.5 2,219,311 - CCNA_02067 0.83 +0.5 2,219,323 + CCNA_02067 0.85 -2.4 2,219,461 - +0.8 2,219,546 - +1.1 2,219,593 + CCNA_02068 0.23 -0.3 2,219,619 + CCNA_02068 0.30 +0.6 2,219,659 + CCNA_02068 0.42 +0.1 2,219,659 + CCNA_02068 0.42 +1.0 2,219,659 + CCNA_02068 0.42 -0.3 2,219,667 - CCNA_02068 0.44 +1.4 2,219,667 - CCNA_02068 0.44 +1.0 2,219,667 - CCNA_02068 0.44 +2.2 2,219,667 - CCNA_02068 0.44 +1.3 2,219,667 - CCNA_02068 0.44 +1.2 2,219,754 + CCNA_02068 0.68 +0.5 2,219,787 + CCNA_02068 0.78 -0.2
Or see this region's nucleotide sequence