Experiment: PYE with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02027 and CCNA_02028 overlap by 4 nucleotides CCNA_02028 and CCNA_02029 overlap by 4 nucleotides CCNA_02029 and CCNA_02030 are separated by 2 nucleotides CCNA_02030 and CCNA_02031 are separated by 21 nucleotides
CCNA_02027: CCNA_02027 - NADH-quinone oxidoreductase chain E, at 2,173,251 to 2,173,937
_02027
CCNA_02028: CCNA_02028 - NTF2 superfamily protein, at 2,173,934 to 2,174,266
_02028
CCNA_02029: CCNA_02029 - NADH-quinone oxidoreductase chain D, at 2,174,263 to 2,175,513
_02029
CCNA_02030: CCNA_02030 - hypothetical protein, at 2,175,516 to 2,175,911
_02030
CCNA_02031: CCNA_02031 - NADH-quinone oxidoreductase chain C, at 2,175,933 to 2,176,526
_02031
Position (kb) 2174
2175
2176 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 2173.948 kb on - strand at 2174.000 kb on - strand, within CCNA_02028 at 2174.159 kb on - strand, within CCNA_02028 at 2175.678 kb on - strand, within CCNA_02030 at 2175.782 kb on - strand, within CCNA_02030 at 2175.810 kb on - strand, within CCNA_02030 at 2175.914 kb on - strand at 2175.941 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.025 mg/ml remove 2,173,948 - +4.1 2,174,000 - CCNA_02028 0.20 +3.9 2,174,159 - CCNA_02028 0.68 +2.6 2,175,678 - CCNA_02030 0.41 +2.5 2,175,782 - CCNA_02030 0.67 +4.8 2,175,810 - CCNA_02030 0.74 +3.0 2,175,914 - +3.2 2,175,941 - +2.2
Or see this region's nucleotide sequence