Experiment: PYE with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02025 and CCNA_02026 overlap by 4 nucleotides CCNA_02026 and CCNA_02027 are separated by 1 nucleotides CCNA_02027 and CCNA_02028 overlap by 4 nucleotides CCNA_02028 and CCNA_02029 overlap by 4 nucleotides
CCNA_02025: CCNA_02025 - hypothetical protein, at 2,172,762 to 2,173,028
_02025
CCNA_02026: CCNA_02026 - hypothetical protein, at 2,173,025 to 2,173,249
_02026
CCNA_02027: CCNA_02027 - NADH-quinone oxidoreductase chain E, at 2,173,251 to 2,173,937
_02027
CCNA_02028: CCNA_02028 - NTF2 superfamily protein, at 2,173,934 to 2,174,266
_02028
CCNA_02029: CCNA_02029 - NADH-quinone oxidoreductase chain D, at 2,174,263 to 2,175,513
_02029
Position (kb) 2173
2174
2175 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 2172.978 kb on - strand, within CCNA_02025 at 2172.978 kb on - strand, within CCNA_02025 at 2173.002 kb on - strand at 2173.185 kb on - strand, within CCNA_02026 at 2173.185 kb on - strand, within CCNA_02026 at 2173.220 kb on - strand, within CCNA_02026 at 2173.234 kb on - strand at 2173.948 kb on - strand at 2174.000 kb on - strand, within CCNA_02028 at 2174.159 kb on - strand, within CCNA_02028
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.025 mg/ml remove 2,172,978 - CCNA_02025 0.81 +1.1 2,172,978 - CCNA_02025 0.81 +2.1 2,173,002 - +2.3 2,173,185 - CCNA_02026 0.71 +2.2 2,173,185 - CCNA_02026 0.71 +2.9 2,173,220 - CCNA_02026 0.87 +2.7 2,173,234 - +2.0 2,173,948 - +4.1 2,174,000 - CCNA_02028 0.20 +3.9 2,174,159 - CCNA_02028 0.68 +2.6
Or see this region's nucleotide sequence