Strain Fitness in Caulobacter crescentus NA1000 around CCNA_00249

Experiment: PYE with Spectinomycin 0.025 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_00248 and CCNA_00249 are separated by 56 nucleotidesCCNA_00249 and CCNA_00250 are separated by 80 nucleotides CCNA_00248: CCNA_00248 - sensor histidine protein kinase, at 259,514 to 260,914 _00248 CCNA_00249: CCNA_00249 - SCO1/SenC family protein, at 260,971 to 261,561 _00249 CCNA_00250: CCNA_00250 - poly(3-hydroxyalkanoate) depolymerase, at 261,642 to 262,904 _00250 Position (kb) 260 261 262Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 259.975 kb on - strand, within CCNA_00248at 259.975 kb on - strand, within CCNA_00248at 259.978 kb on + strand, within CCNA_00248at 259.980 kb on - strand, within CCNA_00248at 259.985 kb on - strand, within CCNA_00248at 259.989 kb on - strand, within CCNA_00248at 259.997 kb on + strand, within CCNA_00248at 260.014 kb on - strand, within CCNA_00248at 260.044 kb on - strand, within CCNA_00248at 260.123 kb on - strand, within CCNA_00248at 260.123 kb on - strand, within CCNA_00248at 260.123 kb on - strand, within CCNA_00248at 260.193 kb on + strand, within CCNA_00248at 260.223 kb on - strand, within CCNA_00248at 260.249 kb on + strand, within CCNA_00248at 260.279 kb on + strand, within CCNA_00248at 260.299 kb on - strand, within CCNA_00248at 260.352 kb on + strand, within CCNA_00248at 260.360 kb on - strand, within CCNA_00248at 260.365 kb on - strand, within CCNA_00248at 260.400 kb on + strand, within CCNA_00248at 260.408 kb on - strand, within CCNA_00248at 260.442 kb on + strand, within CCNA_00248at 260.450 kb on + strand, within CCNA_00248at 260.450 kb on + strand, within CCNA_00248at 260.450 kb on + strand, within CCNA_00248at 260.450 kb on + strand, within CCNA_00248at 260.450 kb on + strand, within CCNA_00248at 260.450 kb on + strand, within CCNA_00248at 260.450 kb on + strand, within CCNA_00248at 260.455 kb on + strand, within CCNA_00248at 260.458 kb on - strand, within CCNA_00248at 260.458 kb on - strand, within CCNA_00248at 260.460 kb on - strand, within CCNA_00248at 260.490 kb on - strand, within CCNA_00248at 260.512 kb on - strand, within CCNA_00248at 260.549 kb on - strand, within CCNA_00248at 260.549 kb on - strand, within CCNA_00248at 260.553 kb on + strand, within CCNA_00248at 260.554 kb on - strand, within CCNA_00248at 260.554 kb on - strand, within CCNA_00248at 260.561 kb on - strand, within CCNA_00248at 260.578 kb on - strand, within CCNA_00248at 260.629 kb on - strand, within CCNA_00248at 260.633 kb on + strand, within CCNA_00248at 260.633 kb on + strand, within CCNA_00248at 260.654 kb on + strand, within CCNA_00248at 260.654 kb on + strand, within CCNA_00248at 260.671 kb on + strand, within CCNA_00248at 260.679 kb on - strand, within CCNA_00248at 260.679 kb on - strand, within CCNA_00248at 260.679 kb on - strand, within CCNA_00248at 260.679 kb on - strand, within CCNA_00248at 260.685 kb on + strand, within CCNA_00248at 260.686 kb on - strand, within CCNA_00248at 260.693 kb on - strand, within CCNA_00248at 260.718 kb on + strand, within CCNA_00248at 260.723 kb on + strand, within CCNA_00248at 260.723 kb on + strand, within CCNA_00248at 260.731 kb on - strand, within CCNA_00248at 260.731 kb on - strand, within CCNA_00248at 260.731 kb on - strand, within CCNA_00248at 260.743 kb on - strand, within CCNA_00248at 260.759 kb on + strandat 260.803 kb on + strandat 260.804 kb on + strandat 260.806 kb on - strandat 260.811 kb on - strandat 260.812 kb on - strandat 260.814 kb on + strandat 260.814 kb on + strandat 260.814 kb on + strandat 260.819 kb on + strandat 260.822 kb on - strandat 260.822 kb on - strandat 260.861 kb on - strandat 260.866 kb on - strandat 260.927 kb on + strandat 260.927 kb on + strandat 260.935 kb on - strandat 260.935 kb on - strandat 260.984 kb on + strandat 260.989 kb on + strandat 260.992 kb on - strandat 261.015 kb on - strandat 261.026 kb on + strandat 261.033 kb on - strand, within CCNA_00249at 261.038 kb on + strand, within CCNA_00249at 261.067 kb on - strand, within CCNA_00249at 261.142 kb on + strand, within CCNA_00249at 261.142 kb on + strand, within CCNA_00249at 261.174 kb on - strand, within CCNA_00249at 261.185 kb on + strand, within CCNA_00249at 261.187 kb on - strand, within CCNA_00249at 261.191 kb on + strand, within CCNA_00249at 261.191 kb on + strand, within CCNA_00249at 261.191 kb on + strand, within CCNA_00249at 261.191 kb on + strand, within CCNA_00249at 261.199 kb on - strand, within CCNA_00249at 261.199 kb on - strand, within CCNA_00249at 261.202 kb on + strand, within CCNA_00249at 261.202 kb on + strand, within CCNA_00249at 261.202 kb on + strand, within CCNA_00249at 261.202 kb on + strand, within CCNA_00249at 261.243 kb on + strand, within CCNA_00249at 261.251 kb on - strand, within CCNA_00249at 261.252 kb on + strand, within CCNA_00249at 261.283 kb on + strand, within CCNA_00249at 261.291 kb on - strand, within CCNA_00249at 261.301 kb on - strand, within CCNA_00249at 261.303 kb on + strand, within CCNA_00249at 261.311 kb on - strand, within CCNA_00249at 261.317 kb on - strand, within CCNA_00249at 261.317 kb on - strand, within CCNA_00249at 261.352 kb on - strand, within CCNA_00249at 261.354 kb on + strand, within CCNA_00249at 261.362 kb on + strand, within CCNA_00249at 261.372 kb on + strand, within CCNA_00249at 261.390 kb on + strand, within CCNA_00249at 261.390 kb on + strand, within CCNA_00249at 261.393 kb on + strand, within CCNA_00249at 261.398 kb on - strand, within CCNA_00249at 261.414 kb on + strand, within CCNA_00249at 261.415 kb on + strand, within CCNA_00249at 261.424 kb on + strand, within CCNA_00249at 261.429 kb on + strand, within CCNA_00249at 261.429 kb on + strand, within CCNA_00249at 261.429 kb on + strand, within CCNA_00249at 261.437 kb on + strand, within CCNA_00249at 261.437 kb on + strand, within CCNA_00249at 261.441 kb on + strand, within CCNA_00249at 261.442 kb on - strand, within CCNA_00249at 261.499 kb on + strand, within CCNA_00249at 261.500 kb on + strand, within CCNA_00249at 261.500 kb on + strand, within CCNA_00249at 261.508 kb on - strandat 261.508 kb on - strandat 261.508 kb on - strandat 261.508 kb on - strandat 261.534 kb on + strandat 261.542 kb on - strandat 261.564 kb on - strandat 261.591 kb on - strandat 261.608 kb on + strandat 261.637 kb on + strandat 261.644 kb on + strandat 261.644 kb on + strandat 261.644 kb on + strandat 261.644 kb on + strandat 261.644 kb on + strandat 261.652 kb on - strandat 261.652 kb on - strandat 261.652 kb on - strandat 261.664 kb on - strandat 261.676 kb on + strandat 261.684 kb on + strandat 261.684 kb on + strandat 261.692 kb on - strandat 261.692 kb on - strandat 261.716 kb on + strandat 261.811 kb on + strand, within CCNA_00250at 261.814 kb on - strand, within CCNA_00250at 261.839 kb on + strand, within CCNA_00250at 261.839 kb on + strand, within CCNA_00250at 261.839 kb on + strand, within CCNA_00250at 261.839 kb on + strand, within CCNA_00250at 261.839 kb on + strand, within CCNA_00250at 261.839 kb on + strand, within CCNA_00250at 261.839 kb on + strand, within CCNA_00250at 261.839 kb on + strand, within CCNA_00250at 261.839 kb on + strand, within CCNA_00250at 261.847 kb on - strand, within CCNA_00250at 261.847 kb on - strand, within CCNA_00250at 261.847 kb on - strand, within CCNA_00250at 261.847 kb on - strand, within CCNA_00250at 261.847 kb on - strand, within CCNA_00250at 261.871 kb on + strand, within CCNA_00250at 261.892 kb on + strand, within CCNA_00250at 261.892 kb on + strand, within CCNA_00250at 261.892 kb on + strand, within CCNA_00250at 261.962 kb on + strand, within CCNA_00250at 261.982 kb on - strand, within CCNA_00250at 261.999 kb on - strand, within CCNA_00250at 262.024 kb on - strand, within CCNA_00250at 262.025 kb on + strand, within CCNA_00250at 262.026 kb on + strand, within CCNA_00250at 262.026 kb on + strand, within CCNA_00250at 262.026 kb on + strand, within CCNA_00250at 262.034 kb on - strand, within CCNA_00250at 262.034 kb on - strand, within CCNA_00250at 262.045 kb on + strand, within CCNA_00250at 262.118 kb on + strand, within CCNA_00250at 262.123 kb on + strand, within CCNA_00250at 262.159 kb on + strand, within CCNA_00250at 262.164 kb on + strand, within CCNA_00250at 262.172 kb on + strand, within CCNA_00250at 262.271 kb on - strand, within CCNA_00250at 262.317 kb on - strand, within CCNA_00250at 262.412 kb on - strand, within CCNA_00250at 262.447 kb on - strand, within CCNA_00250at 262.465 kb on - strand, within CCNA_00250at 262.529 kb on + strand, within CCNA_00250at 262.537 kb on - strand, within CCNA_00250

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE with Spectinomycin 0.025 mg/ml
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259,975 - CCNA_00248 0.33 -0.2
259,975 - CCNA_00248 0.33 +0.6
259,978 + CCNA_00248 0.33 +1.4
259,980 - CCNA_00248 0.33 +0.1
259,985 - CCNA_00248 0.34 +0.3
259,989 - CCNA_00248 0.34 -0.1
259,997 + CCNA_00248 0.34 -0.7
260,014 - CCNA_00248 0.36 -0.2
260,044 - CCNA_00248 0.38 -0.9
260,123 - CCNA_00248 0.43 +0.2
260,123 - CCNA_00248 0.43 +0.2
260,123 - CCNA_00248 0.43 +1.1
260,193 + CCNA_00248 0.48 -0.7
260,223 - CCNA_00248 0.51 +0.2
260,249 + CCNA_00248 0.52 +1.0
260,279 + CCNA_00248 0.55 -0.8
260,299 - CCNA_00248 0.56 +0.7
260,352 + CCNA_00248 0.60 +0.4
260,360 - CCNA_00248 0.60 +0.8
260,365 - CCNA_00248 0.61 -0.9
260,400 + CCNA_00248 0.63 -0.3
260,408 - CCNA_00248 0.64 +0.6
260,442 + CCNA_00248 0.66 -1.9
260,450 + CCNA_00248 0.67 -1.8
260,450 + CCNA_00248 0.67 +0.6
260,450 + CCNA_00248 0.67 -1.2
260,450 + CCNA_00248 0.67 -0.4
260,450 + CCNA_00248 0.67 -0.5
260,450 + CCNA_00248 0.67 -0.8
260,450 + CCNA_00248 0.67 -1.8
260,455 + CCNA_00248 0.67 -2.7
260,458 - CCNA_00248 0.67 -1.9
260,458 - CCNA_00248 0.67 +0.1
260,460 - CCNA_00248 0.68 +0.5
260,490 - CCNA_00248 0.70 +1.0
260,512 - CCNA_00248 0.71 +0.7
260,549 - CCNA_00248 0.74 +0.5
260,549 - CCNA_00248 0.74 -0.5
260,553 + CCNA_00248 0.74 +0.1
260,554 - CCNA_00248 0.74 +0.0
260,554 - CCNA_00248 0.74 -0.7
260,561 - CCNA_00248 0.75 +0.0
260,578 - CCNA_00248 0.76 -0.2
260,629 - CCNA_00248 0.80 -0.5
260,633 + CCNA_00248 0.80 -0.6
260,633 + CCNA_00248 0.80 -0.9
260,654 + CCNA_00248 0.81 -0.2
260,654 + CCNA_00248 0.81 -2.1
260,671 + CCNA_00248 0.83 -0.6
260,679 - CCNA_00248 0.83 -1.4
260,679 - CCNA_00248 0.83 -1.1
260,679 - CCNA_00248 0.83 +1.4
260,679 - CCNA_00248 0.83 +1.4
260,685 + CCNA_00248 0.84 -1.2
260,686 - CCNA_00248 0.84 +0.2
260,693 - CCNA_00248 0.84 -0.4
260,718 + CCNA_00248 0.86 -0.7
260,723 + CCNA_00248 0.86 -1.2
260,723 + CCNA_00248 0.86 -0.0
260,731 - CCNA_00248 0.87 +0.2
260,731 - CCNA_00248 0.87 +0.1
260,731 - CCNA_00248 0.87 +1.8
260,743 - CCNA_00248 0.88 -0.3
260,759 + +1.0
260,803 + +0.3
260,804 + -0.1
260,806 - +0.1
260,811 - +0.6
260,812 - -2.3
260,814 + -0.8
260,814 + -1.5
260,814 + -0.9
260,819 + +0.6
260,822 - -0.6
260,822 - +0.7
260,861 - -0.4
260,866 - -0.5
260,927 + -0.4
260,927 + +1.1
260,935 - -2.3
260,935 - -0.8
260,984 + -0.8
260,989 + -0.4
260,992 - -1.7
261,015 - -1.6
261,026 + -1.2
261,033 - CCNA_00249 0.10 -2.1
261,038 + CCNA_00249 0.11 -1.4
261,067 - CCNA_00249 0.16 +0.4
261,142 + CCNA_00249 0.29 -2.1
261,142 + CCNA_00249 0.29 -1.0
261,174 - CCNA_00249 0.34 -2.3
261,185 + CCNA_00249 0.36 +0.5
261,187 - CCNA_00249 0.37 -1.3
261,191 + CCNA_00249 0.37 -1.6
261,191 + CCNA_00249 0.37 -0.5
261,191 + CCNA_00249 0.37 -0.4
261,191 + CCNA_00249 0.37 +0.1
261,199 - CCNA_00249 0.39 -1.3
261,199 - CCNA_00249 0.39 -1.4
261,202 + CCNA_00249 0.39 -0.0
261,202 + CCNA_00249 0.39 -3.2
261,202 + CCNA_00249 0.39 -0.9
261,202 + CCNA_00249 0.39 -0.3
261,243 + CCNA_00249 0.46 -0.7
261,251 - CCNA_00249 0.47 +0.3
261,252 + CCNA_00249 0.48 +0.1
261,283 + CCNA_00249 0.53 -0.9
261,291 - CCNA_00249 0.54 -0.5
261,301 - CCNA_00249 0.56 +0.2
261,303 + CCNA_00249 0.56 -0.4
261,311 - CCNA_00249 0.58 -0.5
261,317 - CCNA_00249 0.59 +0.5
261,317 - CCNA_00249 0.59 -1.9
261,352 - CCNA_00249 0.64 -0.3
261,354 + CCNA_00249 0.65 +0.3
261,362 + CCNA_00249 0.66 -0.6
261,372 + CCNA_00249 0.68 -0.6
261,390 + CCNA_00249 0.71 -0.4
261,390 + CCNA_00249 0.71 -0.8
261,393 + CCNA_00249 0.71 -1.2
261,398 - CCNA_00249 0.72 -1.3
261,414 + CCNA_00249 0.75 +0.7
261,415 + CCNA_00249 0.75 +0.2
261,424 + CCNA_00249 0.77 -0.3
261,429 + CCNA_00249 0.77 -0.8
261,429 + CCNA_00249 0.77 -0.6
261,429 + CCNA_00249 0.77 -0.9
261,437 + CCNA_00249 0.79 -1.3
261,437 + CCNA_00249 0.79 -0.1
261,441 + CCNA_00249 0.80 +0.2
261,442 - CCNA_00249 0.80 +0.3
261,499 + CCNA_00249 0.89 -1.6
261,500 + CCNA_00249 0.90 -1.2
261,500 + CCNA_00249 0.90 +0.8
261,508 - -0.0
261,508 - -0.2
261,508 - -0.9
261,508 - -0.7
261,534 + -0.4
261,542 - -0.8
261,564 - +0.8
261,591 - +0.4
261,608 + +0.8
261,637 + +0.7
261,644 + +0.2
261,644 + +0.3
261,644 + +0.9
261,644 + -0.1
261,644 + +0.8
261,652 - +1.9
261,652 - -1.1
261,652 - +1.0
261,664 - +1.0
261,676 + +0.9
261,684 + +0.9
261,684 + -0.3
261,692 - -1.6
261,692 - -1.8
261,716 + -0.5
261,811 + CCNA_00250 0.13 -0.2
261,814 - CCNA_00250 0.14 -1.2
261,839 + CCNA_00250 0.16 +0.6
261,839 + CCNA_00250 0.16 +0.2
261,839 + CCNA_00250 0.16 +0.2
261,839 + CCNA_00250 0.16 +1.6
261,839 + CCNA_00250 0.16 +0.8
261,839 + CCNA_00250 0.16 -1.0
261,839 + CCNA_00250 0.16 -0.5
261,839 + CCNA_00250 0.16 +1.6
261,839 + CCNA_00250 0.16 +0.8
261,847 - CCNA_00250 0.16 -0.0
261,847 - CCNA_00250 0.16 +0.5
261,847 - CCNA_00250 0.16 +0.8
261,847 - CCNA_00250 0.16 -0.7
261,847 - CCNA_00250 0.16 -0.0
261,871 + CCNA_00250 0.18 -1.0
261,892 + CCNA_00250 0.20 +1.0
261,892 + CCNA_00250 0.20 +1.1
261,892 + CCNA_00250 0.20 +0.8
261,962 + CCNA_00250 0.25 +0.7
261,982 - CCNA_00250 0.27 -0.9
261,999 - CCNA_00250 0.28 -0.5
262,024 - CCNA_00250 0.30 -0.0
262,025 + CCNA_00250 0.30 +0.2
262,026 + CCNA_00250 0.30 -1.9
262,026 + CCNA_00250 0.30 +0.3
262,026 + CCNA_00250 0.30 -0.6
262,034 - CCNA_00250 0.31 -1.1
262,034 - CCNA_00250 0.31 -1.3
262,045 + CCNA_00250 0.32 +0.2
262,118 + CCNA_00250 0.38 +0.4
262,123 + CCNA_00250 0.38 +0.5
262,159 + CCNA_00250 0.41 -0.8
262,164 + CCNA_00250 0.41 +1.4
262,172 + CCNA_00250 0.42 +0.8
262,271 - CCNA_00250 0.50 -2.0
262,317 - CCNA_00250 0.53 -0.8
262,412 - CCNA_00250 0.61 +1.4
262,447 - CCNA_00250 0.64 +0.2
262,465 - CCNA_00250 0.65 +0.4
262,529 + CCNA_00250 0.70 +0.2
262,537 - CCNA_00250 0.71 -0.6

Or see this region's nucleotide sequence