Experiment: MoYLS4 with Vancomycin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2645 and DvMF_2646 are separated by 105 nucleotides DvMF_2646 and DvMF_2647 are separated by 312 nucleotides DvMF_2647 and DvMF_2648 are separated by 272 nucleotides
DvMF_2645: DvMF_2645 - peptidase U32 (RefSeq), at 3,347,266 to 3,348,558
_2645
DvMF_2646: DvMF_2646 - PHP domain protein (RefSeq), at 3,348,664 to 3,349,548
_2646
DvMF_2647: DvMF_2647 - protein of unknown function DUF343 (RefSeq), at 3,349,861 to 3,350,061
_2647
DvMF_2648: DvMF_2648 - heat shock protein Hsp20 (RefSeq), at 3,350,334 to 3,350,747
_2648
Position (kb)
3348
3349
3350 Strain fitness (log2 ratio)
-2
-1
0
1 at 3347.924 kb on - strand, within DvMF_2645 at 3348.026 kb on + strand, within DvMF_2645 at 3348.098 kb on + strand, within DvMF_2645 at 3348.306 kb on - strand, within DvMF_2645 at 3348.573 kb on + strand at 3348.581 kb on - strand at 3348.696 kb on + strand at 3348.696 kb on + strand at 3348.704 kb on - strand at 3348.838 kb on - strand, within DvMF_2646 at 3348.919 kb on + strand, within DvMF_2646 at 3348.965 kb on + strand, within DvMF_2646 at 3349.405 kb on + strand, within DvMF_2646 at 3349.579 kb on + strand at 3349.587 kb on - strand at 3349.679 kb on + strand at 3349.791 kb on - strand at 3349.846 kb on + strand at 3350.054 kb on - strand at 3350.140 kb on + strand at 3350.186 kb on + strand at 3350.261 kb on + strand at 3350.505 kb on - strand, within DvMF_2648
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 0.25 mg/ml remove 3,347,924 - DvMF_2645 0.51 -1.3 3,348,026 + DvMF_2645 0.59 -1.3 3,348,098 + DvMF_2645 0.64 -1.9 3,348,306 - DvMF_2645 0.80 -1.3 3,348,573 + -1.0 3,348,581 - -0.8 3,348,696 + -0.9 3,348,696 + -0.8 3,348,704 - -0.3 3,348,838 - DvMF_2646 0.20 -0.8 3,348,919 + DvMF_2646 0.29 -1.0 3,348,965 + DvMF_2646 0.34 -0.6 3,349,405 + DvMF_2646 0.84 -1.0 3,349,579 + +0.7 3,349,587 - -0.6 3,349,679 + -0.0 3,349,791 - +0.1 3,349,846 + -0.1 3,350,054 - +0.3 3,350,140 + +0.1 3,350,186 + -0.6 3,350,261 + -0.6 3,350,505 - DvMF_2648 0.41 -0.2
Or see this region's nucleotide sequence