Experiment: MoYLS4 with Vancomycin 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0122 and DvMF_0123 overlap by 1 nucleotides DvMF_0123 and DvMF_0124 are separated by 3 nucleotides DvMF_0124 and DvMF_0125 overlap by 17 nucleotides
DvMF_0122: DvMF_0122 - exodeoxyribonuclease VII, large subunit (RefSeq), at 133,898 to 135,433
_0122
DvMF_0123: DvMF_0123 - Peptidase M23 (RefSeq), at 135,433 to 136,443
_0123
DvMF_0124: DvMF_0124 - exodeoxyribonuclease VII small subunit (RefSeq), at 136,447 to 136,749
_0124
DvMF_0125: DvMF_0125 - Polyprenyl synthetase (RefSeq), at 136,733 to 137,674
_0125
Position (kb) 135
136
137 Strain fitness (log2 ratio) -1
0
1 at 134.700 kb on - strand, within DvMF_0122 at 134.825 kb on - strand, within DvMF_0122 at 135.013 kb on - strand, within DvMF_0122 at 135.024 kb on + strand, within DvMF_0122 at 135.084 kb on + strand, within DvMF_0122 at 135.120 kb on + strand, within DvMF_0122 at 135.121 kb on + strand, within DvMF_0122 at 135.595 kb on - strand, within DvMF_0123 at 135.605 kb on - strand, within DvMF_0123 at 135.676 kb on + strand, within DvMF_0123 at 135.774 kb on - strand, within DvMF_0123 at 136.155 kb on - strand, within DvMF_0123 at 136.607 kb on + strand, within DvMF_0124 at 136.622 kb on + strand, within DvMF_0124 at 137.368 kb on + strand, within DvMF_0125 at 137.403 kb on - strand, within DvMF_0125
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Vancomycin 0.25 mg/ml remove 134,700 - DvMF_0122 0.52 -0.5 134,825 - DvMF_0122 0.60 -0.4 135,013 - DvMF_0122 0.73 +0.1 135,024 + DvMF_0122 0.73 -0.3 135,084 + DvMF_0122 0.77 -0.5 135,120 + DvMF_0122 0.80 -0.5 135,121 + DvMF_0122 0.80 -0.9 135,595 - DvMF_0123 0.16 -0.5 135,605 - DvMF_0123 0.17 +0.0 135,676 + DvMF_0123 0.24 -0.0 135,774 - DvMF_0123 0.34 -0.0 136,155 - DvMF_0123 0.71 +0.2 136,607 + DvMF_0124 0.53 -0.5 136,622 + DvMF_0124 0.58 -0.5 137,368 + DvMF_0125 0.67 +0.2 137,403 - DvMF_0125 0.71 +0.4
Or see this region's nucleotide sequence