Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3724 and Shewana3_3725 are separated by 241 nucleotides Shewana3_3725 and Shewana3_3726 overlap by 1 nucleotides Shewana3_3726 and Shewana3_3727 overlap by 4 nucleotides
Shewana3_3724: Shewana3_3724 - arginase/agmatinase/formiminoglutamase (RefSeq), at 4,466,829 to 4,467,881
_3724
Shewana3_3725: Shewana3_3725 - demethylmenaquinone methyltransferase (RefSeq), at 4,468,123 to 4,468,878
_3725
Shewana3_3726: Shewana3_3726 - hypothetical protein (RefSeq), at 4,468,878 to 4,469,498
_3726
Shewana3_3727: Shewana3_3727 - 2-octaprenylphenol hydroxylase (RefSeq), at 4,469,495 to 4,471,144
_3727
Position (kb)
4468
4469 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4467.178 kb on + strand, within Shewana3_3724 at 4467.183 kb on + strand, within Shewana3_3724 at 4467.191 kb on - strand, within Shewana3_3724 at 4467.270 kb on - strand, within Shewana3_3724 at 4467.444 kb on + strand, within Shewana3_3724 at 4467.463 kb on + strand, within Shewana3_3724 at 4467.700 kb on + strand, within Shewana3_3724 at 4467.738 kb on + strand, within Shewana3_3724 at 4467.755 kb on - strand, within Shewana3_3724 at 4467.778 kb on + strand at 4467.873 kb on + strand at 4467.947 kb on - strand at 4468.077 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 4,467,178 + Shewana3_3724 0.33 +0.5 4,467,183 + Shewana3_3724 0.34 +0.6 4,467,191 - Shewana3_3724 0.34 +0.8 4,467,270 - Shewana3_3724 0.42 -5.1 4,467,444 + Shewana3_3724 0.58 +1.3 4,467,463 + Shewana3_3724 0.60 +0.1 4,467,700 + Shewana3_3724 0.83 +3.1 4,467,738 + Shewana3_3724 0.86 +0.5 4,467,755 - Shewana3_3724 0.88 -0.0 4,467,778 + -0.5 4,467,873 + -0.1 4,467,947 - +2.6 4,468,077 + +0.3
Or see this region's nucleotide sequence