Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3443 and Shewana3_3444 are separated by 8 nucleotides Shewana3_3444 and Shewana3_3445 are separated by 106 nucleotides Shewana3_3445 and Shewana3_3446 are separated by 73 nucleotides
Shewana3_3443: Shewana3_3443 - endonuclease I (RefSeq), at 4,125,663 to 4,126,436
_3443
Shewana3_3444: Shewana3_3444 - 16S ribosomal RNA methyltransferase RsmE (RefSeq), at 4,126,445 to 4,127,176
_3444
Shewana3_3445: Shewana3_3445 - glutathione synthetase (RefSeq), at 4,127,283 to 4,128,230
_3445
Shewana3_3446: Shewana3_3446 - alkaline phosphatase (RefSeq), at 4,128,304 to 4,129,698
_3446
Position (kb)
4127
4128
4129 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4126.320 kb on + strand, within Shewana3_3443 at 4126.351 kb on - strand, within Shewana3_3443 at 4126.510 kb on + strand at 4126.513 kb on - strand at 4126.518 kb on - strand at 4126.535 kb on + strand, within Shewana3_3444 at 4126.640 kb on - strand, within Shewana3_3444 at 4126.791 kb on + strand, within Shewana3_3444 at 4127.005 kb on - strand, within Shewana3_3444 at 4127.108 kb on - strand at 4127.200 kb on + strand at 4127.369 kb on + strand at 4127.369 kb on + strand at 4127.450 kb on + strand, within Shewana3_3445 at 4127.496 kb on + strand, within Shewana3_3445 at 4127.504 kb on - strand, within Shewana3_3445 at 4127.537 kb on - strand, within Shewana3_3445 at 4127.642 kb on - strand, within Shewana3_3445 at 4127.749 kb on + strand, within Shewana3_3445 at 4128.138 kb on + strand at 4128.344 kb on + strand at 4128.586 kb on - strand, within Shewana3_3446 at 4128.778 kb on + strand, within Shewana3_3446 at 4128.962 kb on - strand, within Shewana3_3446 at 4128.984 kb on + strand, within Shewana3_3446
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 4,126,320 + Shewana3_3443 0.85 +2.2 4,126,351 - Shewana3_3443 0.89 +0.2 4,126,510 + -0.3 4,126,513 - +1.0 4,126,518 - +0.4 4,126,535 + Shewana3_3444 0.12 -0.1 4,126,640 - Shewana3_3444 0.27 +1.1 4,126,791 + Shewana3_3444 0.47 +0.9 4,127,005 - Shewana3_3444 0.77 +0.7 4,127,108 - +1.3 4,127,200 + +0.6 4,127,369 + -0.6 4,127,369 + +3.1 4,127,450 + Shewana3_3445 0.18 +1.1 4,127,496 + Shewana3_3445 0.22 +3.6 4,127,504 - Shewana3_3445 0.23 -0.1 4,127,537 - Shewana3_3445 0.27 +0.4 4,127,642 - Shewana3_3445 0.38 +0.5 4,127,749 + Shewana3_3445 0.49 +2.3 4,128,138 + +3.4 4,128,344 + -1.1 4,128,586 - Shewana3_3446 0.20 -1.0 4,128,778 + Shewana3_3446 0.34 +1.0 4,128,962 - Shewana3_3446 0.47 +0.5 4,128,984 + Shewana3_3446 0.49 +1.1
Or see this region's nucleotide sequence