Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3200 and Shewana3_3201 are separated by 385 nucleotides Shewana3_3201 and Shewana3_3202 are separated by 46 nucleotides Shewana3_3202 and djlA are separated by 55 nucleotides
Shewana3_3200: Shewana3_3200 - hypothetical protein (RefSeq), at 3,842,938 to 3,843,972
_3200
Shewana3_3201: Shewana3_3201 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding (RefSeq), at 3,844,358 to 3,845,311
_3201
Shewana3_3202: Shewana3_3202 - hypothetical protein (RefSeq), at 3,845,358 to 3,846,002
_3202
Shewana3_3203: djlA - Dna-J like membrane chaperone protein (RefSeq), at 3,846,058 to 3,846,846
djlA
Position (kb)
3844
3845
3846 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3843.387 kb on + strand, within Shewana3_3200 at 3843.482 kb on - strand, within Shewana3_3200 at 3843.948 kb on - strand at 3843.984 kb on - strand at 3844.124 kb on - strand at 3844.222 kb on - strand at 3844.687 kb on + strand, within Shewana3_3201 at 3844.873 kb on - strand, within Shewana3_3201 at 3844.944 kb on + strand, within Shewana3_3201 at 3844.944 kb on + strand, within Shewana3_3201 at 3844.952 kb on - strand, within Shewana3_3201 at 3845.041 kb on + strand, within Shewana3_3201 at 3845.056 kb on - strand, within Shewana3_3201 at 3845.088 kb on - strand, within Shewana3_3201 at 3845.706 kb on - strand, within Shewana3_3202 at 3845.910 kb on - strand, within Shewana3_3202 at 3845.952 kb on + strand at 3845.959 kb on - strand at 3846.088 kb on + strand at 3846.088 kb on + strand at 3846.088 kb on + strand at 3846.096 kb on - strand at 3846.137 kb on + strand, within djlA
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 3,843,387 + Shewana3_3200 0.43 -2.0 3,843,482 - Shewana3_3200 0.53 -0.6 3,843,948 - -0.5 3,843,984 - +1.6 3,844,124 - -0.4 3,844,222 - +1.5 3,844,687 + Shewana3_3201 0.34 -0.7 3,844,873 - Shewana3_3201 0.54 -0.7 3,844,944 + Shewana3_3201 0.61 +2.0 3,844,944 + Shewana3_3201 0.61 -1.6 3,844,952 - Shewana3_3201 0.62 +1.0 3,845,041 + Shewana3_3201 0.72 -0.4 3,845,056 - Shewana3_3201 0.73 +0.3 3,845,088 - Shewana3_3201 0.77 +0.0 3,845,706 - Shewana3_3202 0.54 +0.4 3,845,910 - Shewana3_3202 0.86 -0.3 3,845,952 + +0.5 3,845,959 - -0.8 3,846,088 + -1.0 3,846,088 + +0.0 3,846,088 + -1.9 3,846,096 - -1.1 3,846,137 + djlA Shewana3_3203 0.10 -0.6
Or see this region's nucleotide sequence