Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3157 and Shewana3_3158 are separated by 50 nucleotides Shewana3_3158 and Shewana3_3159 are separated by 156 nucleotides Shewana3_3159 and Shewana3_3160 overlap by 1 nucleotides
Shewana3_3157: Shewana3_3157 - diguanylate cyclase with GAF sensor (RefSeq), at 3,786,365 to 3,787,627
_3157
Shewana3_3158: Shewana3_3158 - hypothetical protein (RefSeq), at 3,787,678 to 3,788,295
_3158
Shewana3_3159: Shewana3_3159 - hypothetical protein (RefSeq), at 3,788,452 to 3,788,913
_3159
Shewana3_3160: Shewana3_3160 - glutamate-cysteine ligase (RefSeq), at 3,788,913 to 3,790,562
_3160
Position (kb)
3788
3789 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3787.655 kb on - strand at 3787.738 kb on - strand at 3787.748 kb on - strand, within Shewana3_3158 at 3788.000 kb on + strand, within Shewana3_3158 at 3788.008 kb on - strand, within Shewana3_3158 at 3788.008 kb on - strand, within Shewana3_3158 at 3788.010 kb on + strand, within Shewana3_3158 at 3788.018 kb on - strand, within Shewana3_3158 at 3788.081 kb on + strand, within Shewana3_3158 at 3788.089 kb on - strand, within Shewana3_3158 at 3788.342 kb on + strand at 3788.342 kb on + strand at 3788.342 kb on + strand at 3788.350 kb on - strand at 3788.350 kb on - strand at 3788.350 kb on - strand at 3788.350 kb on - strand at 3788.356 kb on - strand at 3788.545 kb on + strand, within Shewana3_3159 at 3788.642 kb on - strand, within Shewana3_3159 at 3788.743 kb on + strand, within Shewana3_3159 at 3788.760 kb on + strand, within Shewana3_3159 at 3788.768 kb on - strand, within Shewana3_3159 at 3788.802 kb on - strand, within Shewana3_3159 at 3788.888 kb on - strand at 3788.888 kb on - strand at 3788.897 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 3,787,655 - -0.8 3,787,738 - +0.4 3,787,748 - Shewana3_3158 0.11 -1.3 3,788,000 + Shewana3_3158 0.52 -0.6 3,788,008 - Shewana3_3158 0.53 -1.0 3,788,008 - Shewana3_3158 0.53 +0.2 3,788,010 + Shewana3_3158 0.54 +1.0 3,788,018 - Shewana3_3158 0.55 +0.2 3,788,081 + Shewana3_3158 0.65 +1.6 3,788,089 - Shewana3_3158 0.67 +1.4 3,788,342 + -0.8 3,788,342 + +1.6 3,788,342 + +0.5 3,788,350 - -1.7 3,788,350 - -3.5 3,788,350 - -0.3 3,788,350 - -0.5 3,788,356 - -0.5 3,788,545 + Shewana3_3159 0.20 -0.3 3,788,642 - Shewana3_3159 0.41 -3.2 3,788,743 + Shewana3_3159 0.63 +0.5 3,788,760 + Shewana3_3159 0.67 -0.5 3,788,768 - Shewana3_3159 0.68 -1.7 3,788,802 - Shewana3_3159 0.76 -1.7 3,788,888 - -3.7 3,788,888 - -4.2 3,788,897 + -0.3
Or see this region's nucleotide sequence