Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2710 and Shewana3_2711 are separated by 472 nucleotides Shewana3_2711 and Shewana3_2712 are separated by 26 nucleotides
Shewana3_2710: Shewana3_2710 - integral membrane protein TerC (RefSeq), at 3,239,622 to 3,240,599
_2710
Shewana3_2711: Shewana3_2711 - hypothetical protein (RefSeq), at 3,241,072 to 3,241,356
_2711
Shewana3_2712: Shewana3_2712 - 3-beta hydroxysteroid dehydrogenase/isomerase (RefSeq), at 3,241,383 to 3,242,567
_2712
Position (kb)
3241
3242 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3240.112 kb on - strand, within Shewana3_2710 at 3240.261 kb on + strand, within Shewana3_2710 at 3240.281 kb on - strand, within Shewana3_2710 at 3240.294 kb on + strand, within Shewana3_2710 at 3240.505 kb on + strand at 3240.674 kb on + strand at 3240.733 kb on + strand at 3240.793 kb on + strand at 3240.881 kb on - strand at 3240.942 kb on + strand at 3240.942 kb on + strand at 3240.942 kb on + strand at 3240.950 kb on - strand at 3241.197 kb on + strand, within Shewana3_2711 at 3241.289 kb on + strand, within Shewana3_2711 at 3241.393 kb on + strand at 3241.424 kb on + strand at 3242.233 kb on - strand, within Shewana3_2712 at 3242.281 kb on + strand, within Shewana3_2712 at 3242.281 kb on + strand, within Shewana3_2712
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 3,240,112 - Shewana3_2710 0.50 +1.6 3,240,261 + Shewana3_2710 0.65 -0.1 3,240,281 - Shewana3_2710 0.67 -0.8 3,240,294 + Shewana3_2710 0.69 +1.7 3,240,505 + +2.2 3,240,674 + -1.7 3,240,733 + -0.1 3,240,793 + -1.5 3,240,881 - -0.2 3,240,942 + +0.2 3,240,942 + -0.4 3,240,942 + -2.4 3,240,950 - -2.2 3,241,197 + Shewana3_2711 0.44 +1.2 3,241,289 + Shewana3_2711 0.76 +2.2 3,241,393 + +0.7 3,241,424 + -1.5 3,242,233 - Shewana3_2712 0.72 -2.1 3,242,281 + Shewana3_2712 0.76 -1.6 3,242,281 + Shewana3_2712 0.76 +0.2
Or see this region's nucleotide sequence