Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt clpX and clpP are separated by 100 nucleotides clpP and tig are separated by 89 nucleotides tig and Shewana3_R0112 are separated by 261 nucleotides Shewana3_R0112 and Shewana3_R0113 are separated by 9 nucleotides Shewana3_R0113 and Shewana3_R0114 are separated by 109 nucleotides Shewana3_R0114 and Shewana3_2663 are separated by 261 nucleotides
Shewana3_2660: clpX - ATP-dependent protease ATP-binding subunit ClpX (RefSeq), at 3,175,465 to 3,176,745
clpX
Shewana3_2661: clpP - ATP-dependent Clp protease proteolytic subunit (RefSeq), at 3,176,846 to 3,177,454
clpP
Shewana3_2662: tig - trigger factor (RefSeq), at 3,177,544 to 3,178,848
tig
Shewana3_R0112: Shewana3_R0112 - tRNA-His (RefSeq), at 3,179,110 to 3,179,185
_R0112
Shewana3_R0113: Shewana3_R0113 - tRNA-Arg (RefSeq), at 3,179,195 to 3,179,271
_R0113
Shewana3_R0114: Shewana3_R0114 - tRNA-Pro (RefSeq), at 3,179,381 to 3,179,457
_R0114
Shewana3_2663: Shewana3_2663 - methenyltetrahydrofolate cyclohydrolase (RefSeq), at 3,179,719 to 3,180,573
_2663
Position (kb)
3177
3178
3179 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3176.643 kb on - strand at 3176.778 kb on - strand at 3176.787 kb on - strand at 3177.085 kb on - strand, within clpP at 3177.104 kb on - strand, within clpP at 3177.398 kb on - strand at 3177.418 kb on - strand at 3177.555 kb on + strand at 3177.691 kb on - strand, within tig at 3177.847 kb on - strand, within tig at 3178.156 kb on - strand, within tig at 3178.901 kb on + strand at 3179.044 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 3,176,643 - +1.2 3,176,778 - +0.3 3,176,787 - +1.2 3,177,085 - clpP Shewana3_2661 0.39 +1.8 3,177,104 - clpP Shewana3_2661 0.42 +1.5 3,177,398 - +1.1 3,177,418 - +1.7 3,177,555 + +0.6 3,177,691 - tig Shewana3_2662 0.11 -1.4 3,177,847 - tig Shewana3_2662 0.23 -1.9 3,178,156 - tig Shewana3_2662 0.47 -2.0 3,178,901 + +0.6 3,179,044 + +2.9
Or see this region's nucleotide sequence