Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2645 and Shewana3_2646 are separated by 94 nucleotides Shewana3_2646 and pspB overlap by 14 nucleotides pspB and Shewana3_2648 are separated by 23 nucleotides Shewana3_2648 and Shewana3_2649 are separated by 177 nucleotides
Shewana3_2645: Shewana3_2645 - protein of unknown function DUF463, YcjX family protein (RefSeq), at 3,159,047 to 3,160,507
_2645
Shewana3_2646: Shewana3_2646 - phage shock protein C, PspC (RefSeq), at 3,160,602 to 3,160,988
_2646
Shewana3_2647: pspB - phage shock protein B (RefSeq), at 3,160,975 to 3,161,208
pspB
Shewana3_2648: Shewana3_2648 - phage shock protein A, PspA (RefSeq), at 3,161,232 to 3,161,915
_2648
Shewana3_2649: Shewana3_2649 - Fis family transcriptional regulator (RefSeq), at 3,162,093 to 3,163,181
_2649
Position (kb)
3160
3161
3162 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3159.992 kb on - strand, within Shewana3_2645 at 3160.173 kb on + strand, within Shewana3_2645 at 3160.411 kb on + strand at 3160.411 kb on - strand at 3160.451 kb on - strand at 3160.913 kb on - strand, within Shewana3_2646 at 3161.036 kb on - strand, within pspB at 3161.044 kb on - strand, within pspB at 3161.103 kb on - strand, within pspB at 3161.108 kb on - strand, within pspB at 3161.148 kb on - strand, within pspB at 3161.247 kb on + strand at 3161.524 kb on - strand, within Shewana3_2648 at 3161.729 kb on + strand, within Shewana3_2648 at 3161.813 kb on + strand, within Shewana3_2648 at 3161.852 kb on - strand at 3161.950 kb on + strand at 3162.158 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 3,159,992 - Shewana3_2645 0.65 -0.5 3,160,173 + Shewana3_2645 0.77 -0.3 3,160,411 + -1.2 3,160,411 - -0.4 3,160,451 - -0.2 3,160,913 - Shewana3_2646 0.80 +0.7 3,161,036 - pspB Shewana3_2647 0.26 +0.0 3,161,044 - pspB Shewana3_2647 0.29 +0.0 3,161,103 - pspB Shewana3_2647 0.55 +2.5 3,161,108 - pspB Shewana3_2647 0.57 +2.0 3,161,148 - pspB Shewana3_2647 0.74 +1.2 3,161,247 + -0.6 3,161,524 - Shewana3_2648 0.43 +1.3 3,161,729 + Shewana3_2648 0.73 +2.2 3,161,813 + Shewana3_2648 0.85 +2.2 3,161,852 - +1.8 3,161,950 + +2.4 3,162,158 - -1.8
Or see this region's nucleotide sequence