Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2504 and Shewana3_2505 are separated by 130 nucleotides Shewana3_2505 and Shewana3_2506 overlap by 14 nucleotides Shewana3_2506 and Shewana3_2507 overlap by 4 nucleotides Shewana3_2507 and Shewana3_2508 are separated by 145 nucleotides
Shewana3_2504: Shewana3_2504 - hypothetical protein (RefSeq), at 2,995,880 to 2,996,272
_2504
Shewana3_2505: Shewana3_2505 - thioesterase superfamily protein (RefSeq), at 2,996,403 to 2,996,867
_2505
Shewana3_2506: Shewana3_2506 - hypothetical protein (RefSeq), at 2,996,854 to 2,997,381
_2506
Shewana3_2507: Shewana3_2507 - histidine triad (HIT) protein (RefSeq), at 2,997,378 to 2,997,734
_2507
Shewana3_2508: Shewana3_2508 - peptidase M61 domain-containing protein (RefSeq), at 2,997,880 to 2,999,646
_2508
Position (kb)
2996
2997
2998 Strain fitness (log2 ratio)
-1
0
1 at 2995.871 kb on + strand at 2995.911 kb on - strand at 2996.354 kb on - strand at 2996.388 kb on + strand at 2996.798 kb on + strand, within Shewana3_2505 at 2996.806 kb on - strand, within Shewana3_2505 at 2996.953 kb on + strand, within Shewana3_2506 at 2996.987 kb on - strand, within Shewana3_2506 at 2996.992 kb on - strand, within Shewana3_2506 at 2997.019 kb on - strand, within Shewana3_2506 at 2997.052 kb on + strand, within Shewana3_2506 at 2997.194 kb on - strand, within Shewana3_2506 at 2997.569 kb on - strand, within Shewana3_2507 at 2997.848 kb on + strand at 2997.968 kb on + strand at 2998.006 kb on - strand at 2998.006 kb on - strand at 2998.031 kb on - strand at 2998.176 kb on + strand, within Shewana3_2508 at 2998.179 kb on + strand, within Shewana3_2508 at 2998.233 kb on - strand, within Shewana3_2508 at 2998.243 kb on + strand, within Shewana3_2508 at 2998.251 kb on - strand, within Shewana3_2508
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 2,995,871 + -0.8 2,995,911 - -0.7 2,996,354 - +0.3 2,996,388 + +0.5 2,996,798 + Shewana3_2505 0.85 -0.2 2,996,806 - Shewana3_2505 0.87 +0.2 2,996,953 + Shewana3_2506 0.19 -1.6 2,996,987 - Shewana3_2506 0.25 -0.0 2,996,992 - Shewana3_2506 0.26 -0.1 2,997,019 - Shewana3_2506 0.31 +1.0 2,997,052 + Shewana3_2506 0.38 -0.7 2,997,194 - Shewana3_2506 0.64 -1.2 2,997,569 - Shewana3_2507 0.54 +0.8 2,997,848 + +0.9 2,997,968 + +0.1 2,998,006 - +0.3 2,998,006 - +1.3 2,998,031 - +1.1 2,998,176 + Shewana3_2508 0.17 +0.0 2,998,179 + Shewana3_2508 0.17 -0.9 2,998,233 - Shewana3_2508 0.20 +0.5 2,998,243 + Shewana3_2508 0.21 -0.5 2,998,251 - Shewana3_2508 0.21 -0.7
Or see this region's nucleotide sequence