Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2475 and Shewana3_2476 are separated by 128 nucleotides Shewana3_2476 and Shewana3_2477 are separated by 287 nucleotides Shewana3_2477 and Shewana3_2478 are separated by 75 nucleotides Shewana3_2478 and Shewana3_2479 are separated by 65 nucleotides
Shewana3_2475: Shewana3_2475 - AMP-binding domain protein (RefSeq), at 2,961,380 to 2,963,104
_2475
Shewana3_2476: Shewana3_2476 - hypothetical protein (RefSeq), at 2,963,233 to 2,963,613
_2476
Shewana3_2477: Shewana3_2477 - hypothetical protein (RefSeq), at 2,963,901 to 2,964,362
_2477
Shewana3_2478: Shewana3_2478 - hypothetical protein (RefSeq), at 2,964,438 to 2,964,776
_2478
Shewana3_2479: Shewana3_2479 - hypothetical protein (RefSeq), at 2,964,842 to 2,965,636
_2479
Position (kb)
2963
2964
2965 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2962.963 kb on + strand at 2962.999 kb on + strand at 2963.218 kb on + strand at 2963.274 kb on + strand, within Shewana3_2476 at 2963.282 kb on - strand, within Shewana3_2476 at 2963.594 kb on - strand at 2963.740 kb on - strand at 2963.812 kb on + strand at 2963.959 kb on - strand, within Shewana3_2477 at 2964.190 kb on + strand, within Shewana3_2477 at 2964.198 kb on - strand, within Shewana3_2477 at 2964.198 kb on - strand, within Shewana3_2477 at 2964.295 kb on + strand, within Shewana3_2477 at 2964.303 kb on - strand, within Shewana3_2477 at 2964.303 kb on - strand, within Shewana3_2477 at 2964.303 kb on - strand, within Shewana3_2477 at 2964.303 kb on - strand, within Shewana3_2477 at 2964.323 kb on - strand at 2964.363 kb on + strand at 2964.395 kb on + strand at 2964.736 kb on + strand, within Shewana3_2478 at 2964.813 kb on - strand at 2964.813 kb on - strand at 2964.847 kb on - strand at 2964.959 kb on + strand, within Shewana3_2479 at 2965.261 kb on - strand, within Shewana3_2479 at 2965.292 kb on + strand, within Shewana3_2479 at 2965.341 kb on + strand, within Shewana3_2479
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 2,962,963 + -0.4 2,962,999 + -0.4 2,963,218 + +1.2 2,963,274 + Shewana3_2476 0.11 -0.1 2,963,282 - Shewana3_2476 0.13 -0.6 2,963,594 - -0.4 2,963,740 - +0.6 2,963,812 + -0.2 2,963,959 - Shewana3_2477 0.13 +0.6 2,964,190 + Shewana3_2477 0.63 -1.1 2,964,198 - Shewana3_2477 0.64 +0.4 2,964,198 - Shewana3_2477 0.64 +0.6 2,964,295 + Shewana3_2477 0.85 -1.8 2,964,303 - Shewana3_2477 0.87 -1.4 2,964,303 - Shewana3_2477 0.87 +0.0 2,964,303 - Shewana3_2477 0.87 -0.1 2,964,303 - Shewana3_2477 0.87 -0.8 2,964,323 - +0.6 2,964,363 + -0.8 2,964,395 + -1.2 2,964,736 + Shewana3_2478 0.88 -1.6 2,964,813 - +2.2 2,964,813 - -2.2 2,964,847 - -0.0 2,964,959 + Shewana3_2479 0.15 -0.5 2,965,261 - Shewana3_2479 0.53 -0.2 2,965,292 + Shewana3_2479 0.57 +2.2 2,965,341 + Shewana3_2479 0.63 +1.2
Or see this region's nucleotide sequence