Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2450 and Shewana3_2451 are separated by 144 nucleotides Shewana3_2451 and Shewana3_2452 are separated by 259 nucleotides Shewana3_2452 and Shewana3_2453 are separated by 163 nucleotides Shewana3_2453 and ushA are separated by 94 nucleotides
Shewana3_2450: Shewana3_2450 - hypothetical protein (RefSeq), at 2,938,533 to 2,939,372
_2450
Shewana3_2451: Shewana3_2451 - hypothetical protein (RefSeq), at 2,939,517 to 2,939,948
_2451
Shewana3_2452: Shewana3_2452 - dihydrouridine synthase, DuS (RefSeq), at 2,940,208 to 2,941,155
_2452
Shewana3_2453: Shewana3_2453 - TraR/DksA family transcriptional regulator (RefSeq), at 2,941,319 to 2,941,678
_2453
Shewana3_2454: ushA - bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor (RefSeq), at 2,941,773 to 2,943,494
ushA
Position (kb)
2940
2941
2942 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2939.345 kb on - strand at 2939.374 kb on + strand at 2939.491 kb on - strand at 2939.589 kb on + strand, within Shewana3_2451 at 2939.731 kb on + strand, within Shewana3_2451 at 2939.811 kb on - strand, within Shewana3_2451 at 2939.900 kb on + strand, within Shewana3_2451 at 2940.044 kb on + strand at 2940.364 kb on - strand, within Shewana3_2452 at 2940.385 kb on - strand, within Shewana3_2452 at 2940.538 kb on + strand, within Shewana3_2452 at 2940.538 kb on + strand, within Shewana3_2452 at 2940.560 kb on - strand, within Shewana3_2452 at 2940.609 kb on + strand, within Shewana3_2452 at 2940.769 kb on - strand, within Shewana3_2452 at 2940.968 kb on + strand, within Shewana3_2452 at 2941.027 kb on - strand, within Shewana3_2452 at 2941.074 kb on + strand at 2941.172 kb on + strand at 2941.172 kb on + strand at 2941.288 kb on + strand at 2941.336 kb on - strand at 2941.443 kb on + strand, within Shewana3_2453 at 2941.538 kb on + strand, within Shewana3_2453 at 2941.610 kb on - strand, within Shewana3_2453 at 2941.787 kb on + strand at 2941.830 kb on + strand at 2941.956 kb on + strand, within ushA at 2942.069 kb on + strand, within ushA
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 2,939,345 - -1.6 2,939,374 + -0.8 2,939,491 - -0.3 2,939,589 + Shewana3_2451 0.17 +1.1 2,939,731 + Shewana3_2451 0.50 -0.1 2,939,811 - Shewana3_2451 0.68 -1.9 2,939,900 + Shewana3_2451 0.89 +0.5 2,940,044 + +2.2 2,940,364 - Shewana3_2452 0.16 -0.1 2,940,385 - Shewana3_2452 0.19 +3.2 2,940,538 + Shewana3_2452 0.35 -0.5 2,940,538 + Shewana3_2452 0.35 -0.3 2,940,560 - Shewana3_2452 0.37 -0.2 2,940,609 + Shewana3_2452 0.42 +0.1 2,940,769 - Shewana3_2452 0.59 -1.1 2,940,968 + Shewana3_2452 0.80 -0.1 2,941,027 - Shewana3_2452 0.86 -2.0 2,941,074 + +1.0 2,941,172 + -1.5 2,941,172 + +0.7 2,941,288 + -0.3 2,941,336 - -0.3 2,941,443 + Shewana3_2453 0.34 -2.6 2,941,538 + Shewana3_2453 0.61 -0.2 2,941,610 - Shewana3_2453 0.81 +0.8 2,941,787 + +1.2 2,941,830 + +0.0 2,941,956 + ushA Shewana3_2454 0.11 +0.2 2,942,069 + ushA Shewana3_2454 0.17 +0.7
Or see this region's nucleotide sequence