Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheT and ihfA are separated by 3 nucleotides ihfA and Shewana3_1866 are separated by 96 nucleotides Shewana3_1866 and Shewana3_1867 are separated by 129 nucleotides Shewana3_1867 and Shewana3_1868 overlap by 1 nucleotides
Shewana3_1864: pheT - phenylalanyl-tRNA synthetase subunit beta (RefSeq), at 2,208,269 to 2,210,656
pheT
Shewana3_1865: ihfA - integration host factor subunit alpha (RefSeq), at 2,210,660 to 2,210,956
ihfA
Shewana3_1866: Shewana3_1866 - lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (RefSeq), at 2,211,053 to 2,211,991
_1866
Shewana3_1867: Shewana3_1867 - hydrogenase nickel insertion protein HypA (RefSeq), at 2,212,121 to 2,212,477
_1867
Shewana3_1868: Shewana3_1868 - hydrogenase expression/formation protein HypE (RefSeq), at 2,212,477 to 2,213,496
_1868
Position (kb)
2211
2212 Strain fitness (log2 ratio)
-2
-1
0
1 at 2211.196 kb on - strand at 2211.523 kb on + strand, within Shewana3_1866 at 2211.569 kb on + strand, within Shewana3_1866 at 2212.204 kb on - strand, within Shewana3_1867 at 2212.209 kb on + strand, within Shewana3_1867 at 2212.494 kb on - strand at 2212.520 kb on - strand at 2212.649 kb on - strand, within Shewana3_1868 at 2212.717 kb on + strand, within Shewana3_1868 at 2212.721 kb on - strand, within Shewana3_1868
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 2,211,196 - +1.4 2,211,523 + Shewana3_1866 0.50 +1.4 2,211,569 + Shewana3_1866 0.55 +0.8 2,212,204 - Shewana3_1867 0.23 -2.1 2,212,209 + Shewana3_1867 0.25 +0.1 2,212,494 - +0.9 2,212,520 - -0.7 2,212,649 - Shewana3_1868 0.17 -1.8 2,212,717 + Shewana3_1868 0.24 +1.4 2,212,721 - Shewana3_1868 0.24 +0.1
Or see this region's nucleotide sequence