Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1750 and Shewana3_1751 are separated by 149 nucleotides Shewana3_1751 and clpS are separated by 193 nucleotides clpS and Shewana3_1753 are separated by 42 nucleotides
Shewana3_1750: Shewana3_1750 - isocitrate dehydrogenase, NADP-dependent (RefSeq), at 2,063,884 to 2,066,109
_1750
Shewana3_1751: Shewana3_1751 - cold-shock DNA-binding protein family protein (RefSeq), at 2,066,259 to 2,066,465
_1751
Shewana3_1752: clpS - ATP-dependent Clp protease adaptor protein ClpS (RefSeq), at 2,066,659 to 2,066,967
clpS
Shewana3_1753: Shewana3_1753 - ATP-dependent Clp protease, ATP-binding subunit clpA (RefSeq), at 2,067,010 to 2,069,277
_1753
Position (kb)
2066
2067 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2065.329 kb on - strand, within Shewana3_1750 at 2065.409 kb on - strand, within Shewana3_1750 at 2065.582 kb on - strand, within Shewana3_1750 at 2065.638 kb on - strand, within Shewana3_1750 at 2065.772 kb on - strand, within Shewana3_1750 at 2065.778 kb on - strand, within Shewana3_1750 at 2065.801 kb on + strand, within Shewana3_1750 at 2065.892 kb on - strand at 2065.897 kb on - strand at 2065.972 kb on - strand at 2065.992 kb on + strand at 2066.102 kb on - strand at 2066.179 kb on - strand at 2066.368 kb on + strand, within Shewana3_1751 at 2066.376 kb on - strand, within Shewana3_1751 at 2066.522 kb on - strand at 2066.548 kb on - strand at 2066.946 kb on + strand at 2066.981 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 2,065,329 - Shewana3_1750 0.65 +2.3 2,065,409 - Shewana3_1750 0.69 +2.3 2,065,582 - Shewana3_1750 0.76 +1.3 2,065,638 - Shewana3_1750 0.79 -0.0 2,065,772 - Shewana3_1750 0.85 +1.3 2,065,778 - Shewana3_1750 0.85 +2.3 2,065,801 + Shewana3_1750 0.86 +3.1 2,065,892 - +2.3 2,065,897 - +0.7 2,065,972 - +2.9 2,065,992 + +2.4 2,066,102 - -1.8 2,066,179 - +2.3 2,066,368 + Shewana3_1751 0.53 -2.5 2,066,376 - Shewana3_1751 0.57 -2.6 2,066,522 - +2.9 2,066,548 - -0.3 2,066,946 + -0.2 2,066,981 + +0.3
Or see this region's nucleotide sequence