Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1665 and Shewana3_1666 are separated by 405 nucleotides Shewana3_1666 and Shewana3_1667 are separated by 109 nucleotides Shewana3_1667 and Shewana3_1668 are separated by 68 nucleotides
Shewana3_1665: Shewana3_1665 - hypothetical protein (RefSeq), at 1,956,112 to 1,957,143
_1665
Shewana3_1666: Shewana3_1666 - butyryl-CoA:acetate CoA transferase (RefSeq), at 1,957,549 to 1,958,205
_1666
Shewana3_1667: Shewana3_1667 - 3-oxoacid CoA-transferase, A subunit (RefSeq), at 1,958,315 to 1,959,019
_1667
Shewana3_1668: Shewana3_1668 - hydroxymethylglutaryl-CoA lyase (RefSeq), at 1,959,088 to 1,960,020
_1668
Position (kb)
1957
1958
1959 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1957.092 kb on + strand at 1957.092 kb on + strand at 1957.247 kb on + strand at 1957.252 kb on + strand at 1957.252 kb on + strand at 1957.354 kb on + strand at 1957.364 kb on - strand at 1957.380 kb on + strand at 1957.387 kb on + strand at 1957.716 kb on - strand, within Shewana3_1666 at 1958.358 kb on + strand at 1959.085 kb on + strand at 1959.093 kb on - strand at 1959.093 kb on - strand at 1959.131 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 1,957,092 + +2.2 1,957,092 + -0.5 1,957,247 + +0.0 1,957,252 + +0.6 1,957,252 + -1.4 1,957,354 + -0.0 1,957,364 - -1.7 1,957,380 + -1.6 1,957,387 + +1.4 1,957,716 - Shewana3_1666 0.25 +1.9 1,958,358 + +0.4 1,959,085 + +2.2 1,959,093 - -0.5 1,959,093 - +0.3 1,959,131 - -2.0
Or see this region's nucleotide sequence