Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt surE and Shewana3_1122 overlap by 4 nucleotides Shewana3_1122 and Shewana3_1123 are separated by 84 nucleotides Shewana3_1123 and Shewana3_1124 are separated by 80 nucleotides
Shewana3_1121: surE - stationary phase survival protein SurE (RefSeq), at 1,319,891 to 1,320,640
surE
Shewana3_1122: Shewana3_1122 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,320,637 to 1,321,272
_1122
Shewana3_1123: Shewana3_1123 - peptidase M23B (RefSeq), at 1,321,357 to 1,322,253
_1123
Shewana3_1124: Shewana3_1124 - RNA polymerase, sigma 38 subunit, RpoS (RefSeq), at 1,322,334 to 1,323,314
_1124
Position (kb) 1321
1322
1323 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1320.397 kb on + strand, within surE at 1320.433 kb on + strand, within surE at 1320.441 kb on - strand, within surE at 1320.578 kb on + strand at 1320.728 kb on + strand, within Shewana3_1122 at 1320.950 kb on + strand, within Shewana3_1122 at 1321.345 kb on - strand at 1321.439 kb on - strand at 1321.544 kb on - strand, within Shewana3_1123 at 1321.878 kb on - strand, within Shewana3_1123 at 1321.922 kb on + strand, within Shewana3_1123 at 1322.101 kb on + strand, within Shewana3_1123 at 1322.243 kb on - strand at 1322.568 kb on + strand, within Shewana3_1124 at 1322.958 kb on - strand, within Shewana3_1124 at 1323.025 kb on - strand, within Shewana3_1124 at 1323.151 kb on + strand, within Shewana3_1124
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 1,320,397 + surE Shewana3_1121 0.67 +0.7 1,320,433 + surE Shewana3_1121 0.72 +0.8 1,320,441 - surE Shewana3_1121 0.73 -0.9 1,320,578 + +0.9 1,320,728 + Shewana3_1122 0.14 -0.6 1,320,950 + Shewana3_1122 0.49 +0.4 1,321,345 - -2.8 1,321,439 - +1.4 1,321,544 - Shewana3_1123 0.21 +2.0 1,321,878 - Shewana3_1123 0.58 -1.0 1,321,922 + Shewana3_1123 0.63 +0.4 1,322,101 + Shewana3_1123 0.83 -1.9 1,322,243 - +2.0 1,322,568 + Shewana3_1124 0.24 -1.0 1,322,958 - Shewana3_1124 0.64 -0.6 1,323,025 - Shewana3_1124 0.70 +0.2 1,323,151 + Shewana3_1124 0.83 +3.7
Or see this region's nucleotide sequence