Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secD and secF are separated by 2 nucleotides secF and Shewana3_1021 are separated by 57 nucleotides Shewana3_1021 and rrmJ are separated by 90 nucleotides rrmJ and Shewana3_1023 are separated by 29 nucleotides
Shewana3_1019: secD - preprotein translocase subunit SecD (RefSeq), at 1,190,220 to 1,192,094
secD
Shewana3_1020: secF - preprotein translocase subunit SecF (RefSeq), at 1,192,097 to 1,192,981
secF
Shewana3_1021: Shewana3_1021 - hypothetical protein (RefSeq), at 1,193,039 to 1,193,338
_1021
Shewana3_1022: rrmJ - 23S rRNA methyltransferase J (RefSeq), at 1,193,429 to 1,194,058
rrmJ
Shewana3_1023: Shewana3_1023 - membrane protease FtsH catalytic subunit (RefSeq), at 1,194,088 to 1,196,061
_1023
Position (kb)
1193
1194 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1192.049 kb on - strand at 1192.056 kb on + strand at 1192.056 kb on + strand at 1192.087 kb on + strand at 1192.095 kb on - strand at 1192.095 kb on - strand at 1192.389 kb on + strand, within secF at 1192.397 kb on - strand, within secF at 1192.447 kb on + strand, within secF at 1192.481 kb on - strand, within secF at 1192.491 kb on - strand, within secF at 1192.498 kb on + strand, within secF at 1192.797 kb on + strand, within secF at 1192.830 kb on + strand, within secF at 1192.945 kb on + strand at 1192.945 kb on + strand at 1192.953 kb on - strand at 1192.955 kb on + strand at 1193.046 kb on + strand at 1193.054 kb on - strand at 1193.256 kb on - strand, within Shewana3_1021 at 1193.280 kb on - strand, within Shewana3_1021 at 1193.686 kb on + strand, within rrmJ
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 1,192,049 - +1.5 1,192,056 + +1.2 1,192,056 + -0.3 1,192,087 + +0.2 1,192,095 - -0.7 1,192,095 - -0.7 1,192,389 + secF Shewana3_1020 0.33 +0.4 1,192,397 - secF Shewana3_1020 0.34 +1.0 1,192,447 + secF Shewana3_1020 0.40 -0.6 1,192,481 - secF Shewana3_1020 0.43 -0.2 1,192,491 - secF Shewana3_1020 0.45 +1.0 1,192,498 + secF Shewana3_1020 0.45 -1.1 1,192,797 + secF Shewana3_1020 0.79 -0.1 1,192,830 + secF Shewana3_1020 0.83 -0.2 1,192,945 + -1.0 1,192,945 + +0.2 1,192,953 - +2.0 1,192,955 + -0.7 1,193,046 + -3.2 1,193,054 - +0.6 1,193,256 - Shewana3_1021 0.72 +1.1 1,193,280 - Shewana3_1021 0.80 +0.4 1,193,686 + rrmJ Shewana3_1022 0.41 +1.6
Or see this region's nucleotide sequence