Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0991 and Shewana3_0992 are separated by 144 nucleotides Shewana3_0992 and Shewana3_0993 overlap by 17 nucleotides Shewana3_0993 and Shewana3_0994 are separated by 17 nucleotides
Shewana3_0991: Shewana3_0991 - penicillin-binding protein 6 (RefSeq), at 1,160,999 to 1,162,174
_0991
Shewana3_0992: Shewana3_0992 - rare lipoprotein A (RefSeq), at 1,162,319 to 1,163,161
_0992
Shewana3_0993: Shewana3_0993 - lytic murein transglycosylase B (RefSeq), at 1,163,145 to 1,164,209
_0993
Shewana3_0994: Shewana3_0994 - rod shape-determining protein RodA (RefSeq), at 1,164,227 to 1,165,330
_0994
Position (kb) 1163
1164
1165 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1162.240 kb on - strand at 1162.310 kb on - strand at 1162.404 kb on - strand, within Shewana3_0992 at 1162.438 kb on + strand, within Shewana3_0992 at 1162.503 kb on - strand, within Shewana3_0992 at 1162.580 kb on - strand, within Shewana3_0992 at 1162.605 kb on + strand, within Shewana3_0992 at 1162.605 kb on + strand, within Shewana3_0992 at 1162.801 kb on - strand, within Shewana3_0992 at 1162.902 kb on + strand, within Shewana3_0992 at 1162.910 kb on - strand, within Shewana3_0992 at 1162.910 kb on - strand, within Shewana3_0992 at 1163.303 kb on - strand, within Shewana3_0993 at 1163.365 kb on - strand, within Shewana3_0993 at 1163.617 kb on + strand, within Shewana3_0993 at 1163.723 kb on - strand, within Shewana3_0993 at 1163.762 kb on + strand, within Shewana3_0993 at 1163.883 kb on - strand, within Shewana3_0993 at 1164.008 kb on + strand, within Shewana3_0993 at 1164.160 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 1,162,240 - +0.1 1,162,310 - -0.6 1,162,404 - Shewana3_0992 0.10 +1.0 1,162,438 + Shewana3_0992 0.14 +0.0 1,162,503 - Shewana3_0992 0.22 +0.0 1,162,580 - Shewana3_0992 0.31 -2.5 1,162,605 + Shewana3_0992 0.34 +2.0 1,162,605 + Shewana3_0992 0.34 +1.0 1,162,801 - Shewana3_0992 0.57 -0.6 1,162,902 + Shewana3_0992 0.69 +2.0 1,162,910 - Shewana3_0992 0.70 +0.0 1,162,910 - Shewana3_0992 0.70 +1.0 1,163,303 - Shewana3_0993 0.15 -1.4 1,163,365 - Shewana3_0993 0.21 -0.4 1,163,617 + Shewana3_0993 0.44 +0.0 1,163,723 - Shewana3_0993 0.54 +0.1 1,163,762 + Shewana3_0993 0.58 +1.0 1,163,883 - Shewana3_0993 0.69 -3.0 1,164,008 + Shewana3_0993 0.81 +2.0 1,164,160 - +0.0
Or see this region's nucleotide sequence