Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0815 and Shewana3_0816 are separated by 92 nucleotides Shewana3_0816 and Shewana3_0817 are separated by 96 nucleotides Shewana3_0817 and Shewana3_0818 are separated by 876 nucleotides
Shewana3_0815: Shewana3_0815 - IS4 family transposase (RefSeq), at 943,094 to 944,707
_0815
Shewana3_0816: Shewana3_0816 - DNA binding domain-containing protein (RefSeq), at 944,800 to 944,979
_0816
Shewana3_0817: Shewana3_0817 - hypothetical protein (RefSeq), at 945,076 to 945,810
_0817
Shewana3_0818: Shewana3_0818 - hypothetical protein (RefSeq), at 946,687 to 947,175
_0818
Position (kb) 945
946 Strain fitness (log2 ratio) -2
-1
0
1 at 944.892 kb on - strand, within Shewana3_0816 at 944.935 kb on - strand, within Shewana3_0816 at 945.013 kb on - strand at 945.088 kb on + strand at 945.541 kb on - strand, within Shewana3_0817 at 945.901 kb on - strand at 945.963 kb on - strand at 946.012 kb on + strand at 946.274 kb on + strand at 946.299 kb on + strand at 946.299 kb on + strand at 946.299 kb on + strand at 946.319 kb on + strand at 946.319 kb on + strand at 946.544 kb on - strand at 946.591 kb on + strand at 946.591 kb on + strand at 946.726 kb on - strand at 946.809 kb on - strand, within Shewana3_0818
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 944,892 - Shewana3_0816 0.51 -0.2 944,935 - Shewana3_0816 0.75 +0.2 945,013 - +0.4 945,088 + -1.3 945,541 - Shewana3_0817 0.63 -1.3 945,901 - -1.3 945,963 - -2.1 946,012 + -0.4 946,274 + +1.3 946,299 + -0.6 946,299 + -0.5 946,299 + -0.8 946,319 + -0.9 946,319 + -0.3 946,544 - -1.0 946,591 + -0.2 946,591 + +0.8 946,726 - -1.4 946,809 - Shewana3_0818 0.25 -0.6
Or see this region's nucleotide sequence