Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0721 and Shewana3_0722 are separated by 34 nucleotides Shewana3_0722 and Shewana3_0723 are separated by 11 nucleotides Shewana3_0723 and Shewana3_0724 are separated by 44 nucleotides Shewana3_0724 and tolC are separated by 72 nucleotides
Shewana3_0721: Shewana3_0721 - enoyl-CoA hydratase (RefSeq), at 838,482 to 839,219
_0721
Shewana3_0722: Shewana3_0722 - hypothetical protein (RefSeq), at 839,254 to 839,736
_0722
Shewana3_0723: Shewana3_0723 - hypothetical protein (RefSeq), at 839,748 to 840,716
_0723
Shewana3_0724: Shewana3_0724 - hypothetical protein (RefSeq), at 840,761 to 841,456
_0724
Shewana3_0725: tolC - outer membrane channel protein (RefSeq), at 841,529 to 842,836
tolC
Position (kb) 839
840
841 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 838.794 kb on + strand, within Shewana3_0721 at 838.796 kb on - strand, within Shewana3_0721 at 838.802 kb on - strand, within Shewana3_0721 at 838.880 kb on - strand, within Shewana3_0721 at 838.890 kb on - strand, within Shewana3_0721 at 838.896 kb on - strand, within Shewana3_0721 at 838.900 kb on - strand, within Shewana3_0721 at 838.905 kb on - strand, within Shewana3_0721 at 839.207 kb on + strand at 839.228 kb on - strand at 839.267 kb on - strand at 839.381 kb on - strand, within Shewana3_0722 at 839.522 kb on + strand, within Shewana3_0722 at 839.909 kb on + strand, within Shewana3_0723 at 840.149 kb on + strand, within Shewana3_0723 at 840.186 kb on - strand, within Shewana3_0723 at 840.186 kb on - strand, within Shewana3_0723 at 840.272 kb on + strand, within Shewana3_0723 at 840.549 kb on - strand, within Shewana3_0723 at 840.593 kb on - strand, within Shewana3_0723 at 840.615 kb on + strand, within Shewana3_0723 at 840.675 kb on + strand at 840.746 kb on + strand at 840.746 kb on + strand at 840.754 kb on - strand at 840.890 kb on + strand, within Shewana3_0724 at 840.995 kb on + strand, within Shewana3_0724 at 841.054 kb on - strand, within Shewana3_0724 at 841.072 kb on + strand, within Shewana3_0724 at 841.092 kb on + strand, within Shewana3_0724 at 841.121 kb on + strand, within Shewana3_0724 at 841.220 kb on + strand, within Shewana3_0724 at 841.220 kb on + strand, within Shewana3_0724 at 841.313 kb on + strand, within Shewana3_0724 at 841.671 kb on - strand, within tolC at 841.671 kb on - strand, within tolC
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 838,794 + Shewana3_0721 0.42 -0.2 838,796 - Shewana3_0721 0.43 +0.7 838,802 - Shewana3_0721 0.43 -1.8 838,880 - Shewana3_0721 0.54 -1.4 838,890 - Shewana3_0721 0.55 -0.6 838,896 - Shewana3_0721 0.56 -0.2 838,900 - Shewana3_0721 0.57 -1.8 838,905 - Shewana3_0721 0.57 +0.2 839,207 + +2.5 839,228 - -0.5 839,267 - -0.9 839,381 - Shewana3_0722 0.26 -1.2 839,522 + Shewana3_0722 0.55 +0.5 839,909 + Shewana3_0723 0.17 -0.7 840,149 + Shewana3_0723 0.41 +0.4 840,186 - Shewana3_0723 0.45 -0.6 840,186 - Shewana3_0723 0.45 -2.3 840,272 + Shewana3_0723 0.54 -0.2 840,549 - Shewana3_0723 0.83 -0.3 840,593 - Shewana3_0723 0.87 +2.0 840,615 + Shewana3_0723 0.89 -1.5 840,675 + +0.7 840,746 + +0.5 840,746 + +0.9 840,754 - +1.0 840,890 + Shewana3_0724 0.19 +0.7 840,995 + Shewana3_0724 0.34 +1.2 841,054 - Shewana3_0724 0.42 +0.4 841,072 + Shewana3_0724 0.45 +0.6 841,092 + Shewana3_0724 0.48 +0.8 841,121 + Shewana3_0724 0.52 +1.2 841,220 + Shewana3_0724 0.66 +0.8 841,220 + Shewana3_0724 0.66 +1.4 841,313 + Shewana3_0724 0.79 +2.1 841,671 - tolC Shewana3_0725 0.11 -5.0 841,671 - tolC Shewana3_0725 0.11 -3.2
Or see this region's nucleotide sequence