Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and Shewana3_0709 are separated by 70 nucleotides Shewana3_0709 and Shewana3_0710 are separated by 13 nucleotides Shewana3_0710 and Shewana3_0711 are separated by 12 nucleotides
Shewana3_0708: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 822,821 to 824,260
thiH
Shewana3_0709: Shewana3_0709 - formate dehydrogenase gamma subunit (RefSeq), at 824,331 to 825,014
_0709
Shewana3_0710: Shewana3_0710 - iron hydrogenase, small subunit (RefSeq), at 825,028 to 825,348
_0710
Shewana3_0711: Shewana3_0711 - response regulator receiver protein (RefSeq), at 825,361 to 826,593
_0711
Position (kb) 824
825
826 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 823.448 kb on - strand, within thiH at 823.499 kb on - strand, within thiH at 823.562 kb on - strand, within thiH at 823.566 kb on + strand, within thiH at 823.574 kb on - strand, within thiH at 823.574 kb on - strand, within thiH at 823.645 kb on - strand, within thiH at 823.692 kb on - strand, within thiH at 823.692 kb on - strand, within thiH at 823.748 kb on + strand, within thiH at 823.753 kb on + strand, within thiH at 823.761 kb on - strand, within thiH at 824.096 kb on + strand, within thiH at 824.104 kb on - strand, within thiH at 824.454 kb on - strand, within Shewana3_0709 at 824.581 kb on - strand, within Shewana3_0709 at 824.726 kb on + strand, within Shewana3_0709 at 824.798 kb on - strand, within Shewana3_0709 at 824.855 kb on + strand, within Shewana3_0709 at 824.855 kb on + strand, within Shewana3_0709 at 824.863 kb on - strand, within Shewana3_0709 at 825.111 kb on - strand, within Shewana3_0710 at 825.253 kb on + strand, within Shewana3_0710 at 825.436 kb on + strand at 825.444 kb on - strand at 825.452 kb on - strand at 825.489 kb on + strand, within Shewana3_0711 at 825.525 kb on - strand, within Shewana3_0711 at 825.560 kb on + strand, within Shewana3_0711 at 825.920 kb on + strand, within Shewana3_0711
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 823,448 - thiH Shewana3_0708 0.44 -1.0 823,499 - thiH Shewana3_0708 0.47 +0.3 823,562 - thiH Shewana3_0708 0.51 +0.4 823,566 + thiH Shewana3_0708 0.52 -0.6 823,574 - thiH Shewana3_0708 0.52 -0.3 823,574 - thiH Shewana3_0708 0.52 +0.1 823,645 - thiH Shewana3_0708 0.57 -0.6 823,692 - thiH Shewana3_0708 0.60 -1.1 823,692 - thiH Shewana3_0708 0.60 -0.3 823,748 + thiH Shewana3_0708 0.64 -0.7 823,753 + thiH Shewana3_0708 0.65 -1.1 823,761 - thiH Shewana3_0708 0.65 -0.1 824,096 + thiH Shewana3_0708 0.89 +0.3 824,104 - thiH Shewana3_0708 0.89 -1.3 824,454 - Shewana3_0709 0.18 +1.1 824,581 - Shewana3_0709 0.37 +0.7 824,726 + Shewana3_0709 0.58 +0.6 824,798 - Shewana3_0709 0.68 +0.1 824,855 + Shewana3_0709 0.77 -1.6 824,855 + Shewana3_0709 0.77 -0.4 824,863 - Shewana3_0709 0.78 +1.6 825,111 - Shewana3_0710 0.26 +2.0 825,253 + Shewana3_0710 0.70 -4.0 825,436 + +1.4 825,444 - -1.4 825,452 - -0.1 825,489 + Shewana3_0711 0.10 -0.7 825,525 - Shewana3_0711 0.13 -0.1 825,560 + Shewana3_0711 0.16 +0.1 825,920 + Shewana3_0711 0.45 -0.4
Or see this region's nucleotide sequence