Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0680 and Shewana3_0681 are separated by 11 nucleotides Shewana3_0681 and Shewana3_0682 are separated by 5 nucleotides Shewana3_0682 and Shewana3_0683 are separated by 16 nucleotides Shewana3_0683 and Shewana3_0684 are separated by 12 nucleotides
Shewana3_0680: Shewana3_0680 - hypothetical protein (RefSeq), at 799,114 to 799,587
_0680
Shewana3_0681: Shewana3_0681 - toluene tolerance family protein (RefSeq), at 799,599 to 800,252
_0681
Shewana3_0682: Shewana3_0682 - SpoIIAA family protein (RefSeq), at 800,258 to 800,542
_0682
Shewana3_0683: Shewana3_0683 - BolA family protein (RefSeq), at 800,559 to 800,810
_0683
Shewana3_0684: Shewana3_0684 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (RefSeq), at 800,823 to 802,082
_0684
Position (kb)
800
801 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 799.303 kb on + strand, within Shewana3_0680 at 799.451 kb on + strand, within Shewana3_0680 at 799.549 kb on + strand at 799.679 kb on + strand, within Shewana3_0681 at 799.687 kb on + strand, within Shewana3_0681 at 799.743 kb on + strand, within Shewana3_0681 at 799.803 kb on + strand, within Shewana3_0681 at 799.811 kb on + strand, within Shewana3_0681 at 799.845 kb on + strand, within Shewana3_0681 at 799.915 kb on + strand, within Shewana3_0681 at 800.089 kb on + strand, within Shewana3_0681 at 800.134 kb on + strand, within Shewana3_0681 at 800.234 kb on + strand at 800.234 kb on + strand at 800.461 kb on + strand, within Shewana3_0682 at 800.618 kb on + strand, within Shewana3_0683
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 799,303 + Shewana3_0680 0.40 -3.7 799,451 + Shewana3_0680 0.71 -2.7 799,549 + -4.6 799,679 + Shewana3_0681 0.12 -0.6 799,687 + Shewana3_0681 0.13 -3.1 799,743 + Shewana3_0681 0.22 -4.0 799,803 + Shewana3_0681 0.31 -2.4 799,811 + Shewana3_0681 0.32 -1.8 799,845 + Shewana3_0681 0.38 -5.5 799,915 + Shewana3_0681 0.48 -4.2 800,089 + Shewana3_0681 0.75 -1.4 800,134 + Shewana3_0681 0.82 -4.0 800,234 + -3.4 800,234 + -4.0 800,461 + Shewana3_0682 0.71 -5.8 800,618 + Shewana3_0683 0.23 +1.4
Or see this region's nucleotide sequence