Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0516 and Shewana3_0517 are separated by 15 nucleotides Shewana3_0517 and Shewana3_0518 are separated by 77 nucleotides
Shewana3_0516: Shewana3_0516 - outer membrane efflux family protein, putative (RefSeq), at 604,751 to 606,121
_0516
Shewana3_0517: Shewana3_0517 - RND family efflux transporter MFP subunit (RefSeq), at 606,137 to 607,417
_0517
Shewana3_0518: Shewana3_0518 - CzcA family heavy metal efflux protein (RefSeq), at 607,495 to 610,707
_0518
Position (kb) 606
607
608 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 605.159 kb on - strand, within Shewana3_0516 at 605.224 kb on + strand, within Shewana3_0516 at 605.234 kb on - strand, within Shewana3_0516 at 605.276 kb on - strand, within Shewana3_0516 at 605.295 kb on + strand, within Shewana3_0516 at 605.303 kb on + strand, within Shewana3_0516 at 605.305 kb on + strand, within Shewana3_0516 at 605.311 kb on - strand, within Shewana3_0516 at 605.326 kb on - strand, within Shewana3_0516 at 605.593 kb on + strand, within Shewana3_0516 at 605.601 kb on - strand, within Shewana3_0516 at 605.628 kb on + strand, within Shewana3_0516 at 605.630 kb on - strand, within Shewana3_0516 at 605.669 kb on - strand, within Shewana3_0516 at 605.669 kb on - strand, within Shewana3_0516 at 605.712 kb on - strand, within Shewana3_0516 at 606.035 kb on - strand at 606.135 kb on - strand at 606.194 kb on + strand at 606.233 kb on + strand at 606.249 kb on + strand at 606.249 kb on + strand at 606.303 kb on - strand, within Shewana3_0517 at 606.442 kb on - strand, within Shewana3_0517 at 606.490 kb on + strand, within Shewana3_0517 at 606.661 kb on + strand, within Shewana3_0517 at 606.727 kb on - strand, within Shewana3_0517 at 606.884 kb on + strand, within Shewana3_0517 at 606.913 kb on + strand, within Shewana3_0517 at 607.031 kb on + strand, within Shewana3_0517 at 607.075 kb on + strand, within Shewana3_0517 at 607.101 kb on - strand, within Shewana3_0517 at 607.144 kb on - strand, within Shewana3_0517 at 607.277 kb on - strand, within Shewana3_0517 at 607.332 kb on - strand at 607.384 kb on + strand at 607.384 kb on + strand at 607.392 kb on - strand at 607.392 kb on - strand at 607.392 kb on - strand at 607.392 kb on - strand at 607.640 kb on + strand at 607.659 kb on - strand at 607.711 kb on + strand at 607.745 kb on + strand at 607.783 kb on - strand at 607.865 kb on + strand, within Shewana3_0518 at 607.895 kb on + strand, within Shewana3_0518 at 608.001 kb on + strand, within Shewana3_0518 at 608.091 kb on + strand, within Shewana3_0518 at 608.230 kb on - strand, within Shewana3_0518 at 608.268 kb on + strand, within Shewana3_0518 at 608.291 kb on + strand, within Shewana3_0518 at 608.301 kb on + strand, within Shewana3_0518
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 605,159 - Shewana3_0516 0.30 -0.3 605,224 + Shewana3_0516 0.35 -0.9 605,234 - Shewana3_0516 0.35 +0.3 605,276 - Shewana3_0516 0.38 -0.6 605,295 + Shewana3_0516 0.40 +0.6 605,303 + Shewana3_0516 0.40 -0.0 605,305 + Shewana3_0516 0.40 +0.9 605,311 - Shewana3_0516 0.41 +0.9 605,326 - Shewana3_0516 0.42 +1.4 605,593 + Shewana3_0516 0.61 -0.5 605,601 - Shewana3_0516 0.62 +0.3 605,628 + Shewana3_0516 0.64 -0.1 605,630 - Shewana3_0516 0.64 +0.5 605,669 - Shewana3_0516 0.67 +0.3 605,669 - Shewana3_0516 0.67 -0.3 605,712 - Shewana3_0516 0.70 +0.5 606,035 - -0.1 606,135 - -0.5 606,194 + -0.9 606,233 + -0.5 606,249 + -0.6 606,249 + -0.5 606,303 - Shewana3_0517 0.13 -1.3 606,442 - Shewana3_0517 0.24 -3.0 606,490 + Shewana3_0517 0.28 -0.8 606,661 + Shewana3_0517 0.41 -0.0 606,727 - Shewana3_0517 0.46 +0.1 606,884 + Shewana3_0517 0.58 +1.3 606,913 + Shewana3_0517 0.61 +0.7 607,031 + Shewana3_0517 0.70 -1.4 607,075 + Shewana3_0517 0.73 +0.2 607,101 - Shewana3_0517 0.75 +1.4 607,144 - Shewana3_0517 0.79 -1.5 607,277 - Shewana3_0517 0.89 +0.1 607,332 - -1.9 607,384 + +1.7 607,384 + -0.8 607,392 - -0.3 607,392 - -0.9 607,392 - -1.7 607,392 - -1.2 607,640 + +0.9 607,659 - +0.2 607,711 + -0.2 607,745 + -0.0 607,783 - -0.2 607,865 + Shewana3_0518 0.12 -1.0 607,895 + Shewana3_0518 0.12 +1.6 608,001 + Shewana3_0518 0.16 -0.6 608,091 + Shewana3_0518 0.19 -0.2 608,230 - Shewana3_0518 0.23 +0.4 608,268 + Shewana3_0518 0.24 -0.6 608,291 + Shewana3_0518 0.25 -2.3 608,301 + Shewana3_0518 0.25 -0.2
Or see this region's nucleotide sequence